CRISPR/Cas9 Gene Targeting in Fertilized Eggs (Bold Fields Required)
PI Last Name:
IACUC Protocol Number:
(default is Cas9 nuclease)
Type of Targeting Vector:
supercoiled circular plasmid
Size of Targeting Vector:
Mouse Strain to Inject:
FVB + $1500
C57BL/6J + $1500
Other + $1500 or more
If other, describe below.
Are null homozygotes sick/infertile/dead?
If yes, explain below.
Building and Room to Deliver Mice to:
NIH Recombinant DNA Regulatory Information
If you have an approved CWRU IBC protocol number enter it here.
If you have an approved CWRU IBC protocol number for this work, enter it. If you do not, answer all the following questions.
Describe the scientific purpose and design of these experiments.
Describe the goals in a way which would be comprehensible to the public.
Is the gene or mutation associated with infectious disease in healthy adult humans?
Are the hazards to personnel and the environment minimal or absent?
Which gene is targeted gene in mice (e.g. beta-actin)
Describe the function of the gene targeted in mice (e.g. cytoskeleton)
Upload a pdf diagram of the targeting strategy