Ovary cryopreservation
We recommend embryo cryopreservation for almost all our customers. Please consult with us first before placing an order for ovary cryopreservation. Our service entails removing the ovaries from
young females, cutting each ovary in half, and freezing each ovary half in
a separate vial by a step-down, slow freezing procedure. Retrieval is a separate
service, and involves transplanting each half ovaries into an ovariectomized
female of an appropriate strain.
What is a test thaw?
We test if live born mice can be produced from
the frozen ovaries.
What is the success rate of retrieval?
Each ovary half has about a 50% probability
of producing live born offspring. Given this rate of success, we recommend
that you provide us with eight female mice for cryopreservation.
What are the important parameters for success?
The younger the female donors, the higher the
probability of recovering live born offspring. In fact, fetal ovaries work
best.
It is also important that the recipient females
are histocompatible with the ovaries. Therefore, we need accurate information
about the strain background. If no matched host is available, we will transplant
into nude mice, but this is not the preferred route.
WHAT YOU WILL PROVIDE
An account number.
Eight female mice.
Accurate strain/background information.
WHAT WE WILL PROVIDE
Surgery to remove ovaries.
Cryopreservation of ovary halves, one per vial.
Test thaw of four vials, transplantation to four hosts, mated and taken to birth.