Transgenic Archive 2000

From hofeldtk at hhmi.org Mon Jan 3 14:22:56 2000
From: hofeldtk at hhmi.org (Hofeldt, Kathryn)
Date: Wed Aug 20 11:55:22 2003
Subject: FW: Job Opportunity - Re-advertisement
Message-ID: <7EC621FFC2DFD2119FFF00805FA7C54F3E4CC6@exchange1.hhmi.org>

> Research Technician 3
> Howard Hughes Medical Institute
> Vanderbilt University Medical School
> Nashville, Tennessee
>
> The Howard Hughes Medical Institute located at Vanderbilt University
> Medical
> School invites applications for a research technician position in Dr.
> Brigid
> Hogan's laboratory. Experience is preferred in generating and maintaining
> transgenic mice by DNA and/or ES cell microinjection, culture of ES cells,
> microsurgery, animal husbandry and embryo dissection and gene expression.
> Good organizational and technical skills and an interest in mammalian
> reproduction and development essential. BS required, MS preferred,
> minimum
> 2 years experience. Training can be given to the right candidate.
>
> The Howard Hughes Medical Institute is a scientific philanthropic
> organization whose principal purpose is the direct conduct of biomedical
> research. Its laboratories are located throughout the U.S. For more
> information about the Institute, please see http://www.hhmi.org.
>
> For more specific information regarding Dr. Hogan's research, please see
> http://www.mc.vanderbilt.edu/vumcdept/cellbio/hogan/home.html
>
> Interested applicants may send their curriculum vitae and salary
> requirements to Angela
> Land-Dedrick, VUMC, 1161 21st Ave South, C2310 MCN, Nashville, TN
> 37232-2175
> or fax to 615-343-2033.
>
> EOE
>
>
>
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From pkiela at peds.arizona.edu Mon Jan 3 12:59:35 2000
From: pkiela at peds.arizona.edu (Pawel R. Kiela)
Date: Wed Aug 20 11:55:23 2003
Subject: Workshops
Message-ID: <002b01bf5625$0f4f57e0$97a3c480@peds.arizona.edu>

Hi all,
Does anybody know about workshops on transgenics that are regularly scheduled in the USA ?
I'd appreciate your help.

Pawel

Pawel R. Kiela, D.V.M., Ph.D.
University of Arizona
Department of Pediatrics
1501 N. Campbell Ave.
Tucson, AZ 85724
Ph. (520) 626 7050
Fax: (520) 626 4141

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From WawrousE at intra.nei.nih.gov Mon Jan 3 15:29:34 2000
From: WawrousE at intra.nei.nih.gov (Wawrousek, Eric (NEI))
Date: Wed Aug 20 11:55:23 2003
Subject: Alternatives to embryo transplant surgery?????
Message-ID: <1B3DC68C3FACD31193B200A0C94A4CE20E0557@NEI1701E>

Hello and Happy New Year to all,

I am in the process of renewing my ACUC protocol and there are two
places where I must list possible alternatives to painful procedures (whether
performed with or without anesthesia). My only procedure in this category is
blastocyst transfer into uterus. Is anyone out there aware of a procedure which
is less traumatic than the standard surgical technique performed under surgical
anesthesia???? I doubt there is anything, but if there is, can you please send
a reference.

Thanks,
EricW
******************************************************
Eric Wawrousek, Ph.D.
Chief, Transgenic Animal and Genome Manipulation Section
National Eye Institute
NIH Bldg. 6 Room 218 phone: 301-496-8841
6 Center Dr. MSC 2730 fax: 301-402-3603
Bethesda, MD 20892-2730 e-mail: ericw@helix.nih.gov

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tjf at uci.edu Tue Jan 4 15:52:13 2000
From: tjf at uci.edu (Tom Fielder)
Date: Wed Aug 20 11:55:23 2003
Subject: failed rederivation of mice
Message-ID: <v04003a08b497c2e89ce2@[128.200.21.145]>

I did my first rederivation procedure last month, and it appears to have
failed completely. Out of 88 2-cell embryos implanted into 4 foster moms,
I have zero pups! I'm wondering if I just made a stupid mistake. Here's
what I did:

The embryo donors (MHV-infected females - not sure what the original strain
was, but these were from a second-generation backcross to C57BL/6) were
superovulated as usual, and fertilized eggs harvested the morning after
mating with C57BL/6 males. The eggs were washed through 5 dishes of FHM
medium with pen/strep, and then incubated overnight in KSOM with pen/strep.
Only those embryos that had gone to the 2-cell phase were implanted into
1.5 day p.c. ICR foster moms. According to the animal technician who takes
care of the quarantined mice, the fosters were not obviously pregnant at
their last cage change, which was 4 days prior to their due date. As far
as I know, there were no signs of dead or cannibalized pups, which should
have been born 2 days ago.

I am not sure exactly what percentage of the eggs went 2-cell, although I
know it was fairly high, probably about 80%. The only reagent that had not
been used previously for mouse embryo work was the pen/strep, which I used
at the standard tissue-culture concentration (i.e., I bought 100X stock and
diluted it 100-fold).

Hopefully something obvious will occur to someone out there in tg-land, but
it has me stumped.

Tom

*******************************************
Thomas J. Fielder, M.A., Specialist
Transgenic Mouse Facility
UC-Irvine
University Lab Animal Resources
1315 Bio Sci II
Irvine, CA 92697-1310
email: tjf@uci.edu
phone: 949-824-8579
fax: 949-824-2003
http://darwin.bio.uci.edu/~tjf/index.html
*******************************************

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From ssp at its.caltech.edu Tue Jan 4 16:12:49 2000
From: ssp at its.caltech.edu (Shirley Pease)
Date: Wed Aug 20 11:55:23 2003
Subject: failed rederivation of mice
In-Reply-To: <v04003a08b497c2e89ce2@[128.200.21.145]>
Message-ID: <Pine.GSO.4.05.10001041612110.3082-100000@sue>

Hi Tom,

I would have implanted the 2 cell embryos into day 0.5 recips. I wonder
if that was your problem?

Best, Shirley.

Shirley Pease,
Director, Transgenic and Knockout Core Facility,
California Institute of Technology,
Division of Biology, 147-75
Pasadena,
California, 91125
Tel 626 395 3996
Fax 626 449 0756

On Tue, 4 Jan 2000, Tom Fielder wrote:

> I did my first rederivation procedure last month, and it appears to have
> failed completely. Out of 88 2-cell embryos implanted into 4 foster moms,
> I have zero pups! I'm wondering if I just made a stupid mistake. Here's
> what I did:
>
> The embryo donors (MHV-infected females - not sure what the original strain
> was, but these were from a second-generation backcross to C57BL/6) were
> superovulated as usual, and fertilized eggs harvested the morning after
> mating with C57BL/6 males. The eggs were washed through 5 dishes of FHM
> medium with pen/strep, and then incubated overnight in KSOM with pen/strep.
> Only those embryos that had gone to the 2-cell phase were implanted into
> 1.5 day p.c. ICR foster moms. According to the animal technician who takes
> care of the quarantined mice, the fosters were not obviously pregnant at
> their last cage change, which was 4 days prior to their due date. As far
> as I know, there were no signs of dead or cannibalized pups, which should
> have been born 2 days ago.
>
> I am not sure exactly what percentage of the eggs went 2-cell, although I
> know it was fairly high, probably about 80%. The only reagent that had not
> been used previously for mouse embryo work was the pen/strep, which I used
> at the standard tissue-culture concentration (i.e., I bought 100X stock and
> diluted it 100-fold).
>
> Hopefully something obvious will occur to someone out there in tg-land, but
> it has me stumped.
>
> Tom
>
> *******************************************
> Thomas J. Fielder, M.A., Specialist
> Transgenic Mouse Facility
> UC-Irvine
> University Lab Animal Resources
> 1315 Bio Sci II
> Irvine, CA 92697-1310
> email: tjf@uci.edu
> phone: 949-824-8579
> fax: 949-824-2003
> http://darwin.bio.uci.edu/~tjf/index.html
> *******************************************
>
>
>
> "transgenic-list" web site (archives, FAQs, protocols, links) address:
> http://www.med.ic.ac.uk/db/dbbm/tglist.htm
>
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From silvacub at its.caltech.edu Tue Jan 4 16:33:29 2000
From: silvacub at its.caltech.edu (Juan C. Silva)
Date: Wed Aug 20 11:55:23 2003
Subject: failed rederivation of mice
In-Reply-To: <v04003a08b497c2e89ce2@[128.200.21.145]>
Message-ID: <Pine.GSO.4.05.10001041631580.7600-100000@inky>

Hi Tom,
The only thing I would do different is to implant the embryos into 0.5
females.
Juan

<:3_)~~~ <:3_)~~~ <:3_)~~~ <:3_)~~~ <:3_)~~~ <:3_)~~~ <:3_)~~~

Juan Carlos Silva
Division of Biology
Cryopreservation, Micro-injection and Embryonic Stem Cell Facility
California Institute of Technology 147-75
1200 East California Blvd _ _
Pasadena, CA 91125 (_)-(_)
Tel: (626) 395-2469 \"/
Fax: (626) 844-3402 =V=
silvacub@cco.caltech.edu

On Tue, 4 Jan 2000, Tom Fielder wrote:

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From josepha at amgen.com Wed Jan 5 08:19:24 2000
From: josepha at amgen.com (Anderson, Joe)
Date: Wed Aug 20 11:55:23 2003
Subject: Failed rederivations
Message-ID: <401D3C121A10D3118A7F0008C7CF080E2C6AC6@tan-exch.amgen.com>

Good morning Tom,

I agree with Juan about the recipients. Keep in mind that embryo
development in culture will be retarded compared to in vivo. Transferring
1.5 day cultured embryos into 1.5 day PsPg recipients will actually result
in the recipient PsPg being ahead of the embryo development and the
pregnancy will fail. Transferring the fertile one cell embryos on the same
collection day will also work well.

Another suggestion: It is unnecessary to transfer more than about
12-15 un-manipulated embryos (one cell or two cell) for a "normal"
pregnancy. You will need at least 6 implanted embryos to establish
pregnancy, however.

Hope this is of some help.

Joe Anderson
Josepha@amgen.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jdl at po.cwru.edu Wed Jan 5 11:50:16 2000
From: jdl at po.cwru.edu (Jackie Nicholson)
Date: Wed Aug 20 11:55:23 2003
Subject: failed rederivations
Message-ID: <l03130307b49926338cb3@[129.22.89.103]>

>Good morning Tom,
>
> I agree with Juan about the recipients. Keep in mind that embryo
>development in culture will be retarded compared to in vivo. Transferring
>1.5 day cultured embryos into 1.5 day PsPg recipients will actually result
>in the recipient PsPg being ahead of the embryo development and the
>pregnancy will fail. Transferring the fertile one cell embryos on the same
>collection day will also work well.

Wasn't there just a discussion about MHV needing an O/N incubation for
rederivation?

I agree with all others, 0.5d recipients should have been used.

******************************************
Jackie Nicholson, Manager
Core Transgenic Facility
Case Western Reserve University
10900 Euclid Avenue
Cleveland, Ohio 44106-4955
(216)844-7971 fax(216)368-3432 jdl@po.cwru.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tsaunder at umich.edu Wed Jan 5 13:10:12 2000
From: tsaunder at umich.edu (Thom Saunders)
Date: Wed Aug 20 11:55:23 2003
Subject: failed rederivation of mice
In-Reply-To: <v04003a08b497c2e89ce2@[128.200.21.145]>
Message-ID: <66532.3156066612@host-83.subnet-31.med.umich.edu>

Hi Tom,

I agree with the others, if you had used day 0.5 recipients you would have
had pregnancies.

I believe that it is mycoplasma that requires the overnight incubation in a
cleanup. Media without serum, such as KSOM and M16 don't provide mycoplasma
with enough nutrients to servive an overnight incubation.

Sincerely,

Thom Saunders, Ph.D.
Transgenic Animal Model Core
University of Michigan Medical School
Ann Arbor, MI 48109-0674

email: tsaunder@umich.edu
URL: http://www.med.umich.edu/tamc/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jdl at po.cwru.edu Wed Jan 5 14:30:13 2000
From: jdl at po.cwru.edu (Jackie Nicholson)
Date: Wed Aug 20 11:55:23 2003
Subject: mycoplasma not MHV
Message-ID: <l0313030fb4994be0681e@[129.22.89.103]>

Sorry list! I checked my archives, it was mycoplasma that requires an
overnight culture, not MHV.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jklohse at facstaff.wisc.edu Wed Jan 5 18:06:30 2000
From: jklohse at facstaff.wisc.edu (Jan K. Lohse)
Date: Wed Aug 20 11:55:23 2003
Subject: failed rederivation of mice
In-Reply-To: <v04003a08b497c2e89ce2@[128.200.21.145]>
Message-ID: <v04011702b4998c1ea0ff@[128.104.215.81]>

>The embryo donors were
>superovulated as usual, and fertilized eggs harvested the morning after
>mating with C57BL/6 males. The eggs were washed through 5 dishes of FHM
>medium with pen/strep, and then incubated overnight in KSOM with pen/strep.
>Only those embryos that had gone to the 2-cell phase were implanted into
>1.5 day p.c. ICR foster moms. According to the animal technician who takes
>care of the quarantined mice, the fosters were not obviously pregnant at
>their last cage change, which was 4 days prior to their due date. As far
>as I know, there were no signs of dead or cannibalized pups, which should
>have been born 2 days ago.

My best guess would be that the embryos, after going through overnight
culture, were too far delayed for successful implantation into day 1.5 p.c.
recips. Day 0.5 recips would have been more appropriate.

Dr. Jan K. Lohse
Senior Research Specialist
Department of Pathobiological Sciences
School of Veterinary Medicine
2015 Linden Drive West
Madison, WI 53706
(608) 263-9748

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From Kristina.Vintersten at EMBL-Heidelberg.de Thu Jan 6 08:26:53 2000
From: Kristina.Vintersten at EMBL-Heidelberg.de (Kristina Vintersten)
Date: Wed Aug 20 11:55:23 2003
Subject: failed rederivation of mice
In-Reply-To: <66532.3156066612@host-83.subnet-31.med.umich.edu>
References: <v04003a08b497c2e89ce2@[128.200.21.145]>
Message-ID: <l03130300b499e4cd9adf@[194.94.44.118]>

Dear Tom,

I have just had a big discussion here about Mycoplasma, and I am therefore
very interested in getting other peoples opinions/experiences. Could you
tell me more about your theory that M16/KSOM would not provide mycoplasma
with enough nutrients to servive an overnight incubation? Can you prove it,
is there a reference?
Maybe you also have an ideas about injecting Mycoplasma contaminated
ES-cells? Is there a risk that the chimeric offspring will get
contaminated? I would very much appreciate to get responses from people who
have done this, and screened the offspring for Mycoplasma.

Best regards

Kristina

>Hi Tom,
>
>I agree with the others, if you had used day 0.5 recipients you would have
>had pregnancies.
>
>I believe that it is mycoplasma that requires the overnight incubation in a
>cleanup. Media without serum, such as KSOM and M16 don't provide mycoplasma
>with enough nutrients to servive an overnight incubation.
>
>Sincerely,
>
>Thom Saunders, Ph.D.
>Transgenic Animal Model Core
>University of Michigan Medical School
>Ann Arbor, MI 48109-0674
>
>email: tsaunder@umich.edu
>URL: http://www.med.umich.edu/tamc/
>
>
>"transgenic-list" web site (archives, FAQs, protocols, links) address:
>http://www.med.ic.ac.uk/db/dbbm/tglist.htm

______________________________________________________________________

EMBL Transgenic Service
Kristina Vintersten Phone : +49 / 6221 / 387 514
Meyerhofstr. 1 Fax : +49 / 6221 / 387 306
D-69012 Heidelberg E-mail: vinterst@embl-heidelberg.de
GERMANY

http://www.embl-heidelberg.de/ExternalInfo/transgenicService/

_ _
(_)-(_)
_________________________ \"/ ________________________________________
=V=

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tjf at uci.edu Thu Jan 6 09:28:04 2000
From: tjf at uci.edu (Tom Fielder)
Date: Wed Aug 20 11:55:23 2003
Subject: summary of rederivation responses
Message-ID: <v04003a02b49a0bf30fb3@[128.200.21.145]>

Thanks to all who responded to my question about the failed mouse
rederivation. I really appreciate all the comments, but there were just
too many to respond to each one individually. The overwhelming consensus
was that I should have used 0.5d p.c. fosters. In self-defense, I had
originally planned to do that, but changed my plans because I did not want
to re-enter the vivarium after harvesting eggs in the quarantine room, and
there was no one else available who was trained to set up pseudopregs based
on signs of estrus.

The main problem with using 1.5 d p.c. fosters for transfer of 2-cell
embryos, according to those who voiced a theory, seems to be that the
embryos are delayed by overnight culture, and therefore the fosters'
reproductive systems were too advanced to accept the embryos. A few people
also suggested that, because of the partial C57BL/6 background of the
embryos, they would have undergone 2-cell stage blockage.

Also, the majority of respondents felt it was unnecessary to do the
overnight incubation, since mycoplasma was not involved. In retrospect, if
I had not decided to do that, I could have set up the fosters as originally
planned and everything probably would have been fine. Such is life, and I
can only hope that this public airing of my mistake will help prevent
others from repeating it.

Tom

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tjf at uci.edu Thu Jan 6 11:37:12 2000
From: tjf at uci.edu (Tom Fielder)
Date: Wed Aug 20 11:55:23 2003
Subject: cryopreservation fees survey
Message-ID: <v04003a08b49a2ac64dca@[128.200.21.145]>

I'm about to offer cryopreservation of mouse embryos as my newest service
and would appreciate some feedback on several questions regarding recharge
rates. These relate only to 8-cell stage embryos. I'll leave sperm for
another day.

What do you charge for freezing 8-cell stage mouse embryos?

How many donors and/or studs do you ask the client to supply?

How do you recover the storage costs (e.g., liquid nitrogen, labor, etc.) -
is this billed on a yearly basis, or do you charge it as one lump sum up
front?

What do you charge for reconstituting a frozen line? Is this billed as a
separate service?

Do your fees vary depending on the strain of mouse?

Please reply directly to me. I'll summarize responses anonymously for the
list, giving a range and average in the interests of saving time. However,
I would also like to put this information on my website eventually, showing
all the details for each facility (like I did for DNA microinjection and ES
cell injection) so if you want it to remain anonymous there as well, please
let me know.

Thanks in advance.

Tom

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From cbrayton at bcm.tmc.edu Fri Jan 7 12:42:04 2000
From: cbrayton at bcm.tmc.edu (Dr. Cory Brayton)
Date: Wed Aug 20 11:55:23 2003
Subject: Conference/Course Announcement
Message-ID: <NDBBLJPMFKHCFAODHJDHGEIJCCAA.cbrayton@bcm.tmc.edu>

PATHOLOGY OF MUTANT ANIMAL MODELS 2000
Sponsored by the C. L. Davis Foundation for the Advancement of Veterinary
and Comparative Pathology at
Baylor College of Medicine, Houston, Texas
February 17-19, 2000

Aims
1) Establish Dialogue between Comparative Pathologists, Veterinarians and
Investigators.
2) Provide forum for investigators, postdoctoral trainees and graduate
students to be introduced to Comparative Pathology.
3) Provide forum for discussing different approaches to evaluating mutant
animals as potential animal models -- these vary with interest and expertise
of the institutions.
4) Provide forum for Comparative Pathologists in training, and those
interested in Comparative Pathology (DVM, PhD, MD) to present cases and be
exposed to different approaches to phenotype characterization.

It has been posted on a couple of NIH websites including
http://www.nih.gov/science/models/mouse/courses/pathmut2000.html

Details including registration forms are at
http://www.afip.org/CLDavis/CLDavis.meetings.htm

Available space & hotel rooms are filling fast -- housing alternatives &
travel suggestions are provided at the bottom of this message.
The Submission deadline for case presentations has been extended to January
15.

Below is the unformatted Announcement and Schedule -- not very pretty but
you should not have trouble opening/reading it.

Please contact me (Cory Brayton) if you would like additional information or
if you would like to have the registration form faxed to you.

-----------------------------------------------------------

Thursday, February 17, 2000
730am Registration Coffee & Donuts
830 Dr. Cory Brayton Introduction and Welcome
845 Dr. Charles Montgomery Comparative Pathology, its role in evaluation
of mutant animals
1030 Dr. Bruce Car Rodent Clinical Pathology
noon Lunch
1-5 pm Case Presentations
630 Dinner

Friday, February 18, 2000
730am Registration Coffee & Donuts
800 Dr. Peter Mann Background Lesions and the Significance of Strain
variability
915 Dr. Jerry Ward Early Embryonic Death and Neonatal Lethality
1045 Dr. Marilyn Wolfe Fish Pathology Systematic Evaluation, toxicologic
perspective
noon Lunch
1-5pm Case Presentations

Saturday, February 19, 2000
830am Dr. John Belmont Approach to Immunophenotyping
10 Dr. Lloyd Michael Approach to Cardiovascular Phenotyping
Noon Conclusions
------------------------------
Registration Fees
The registration fee includes the cost of the course, lunches, Thursday
dinner, and 17 hours of continuing education.
Davis Foundation Members: $200.00 ($250.00 After 12/31/99)
Non-members: $275.00* ($325.00 After 12/31/99)
Extra Dinner Guests/Spouses: $ 25.00
*Cost to new members includes 2000 membership, and T60 videotutorial
entitled ?Laboratory Animal Diseases?

Lodging: Medical Center Marriott - 713 796-0080
($99.00/d Request BCM/CLDavis)
Also Check
Crown Plaza 713-797-1110 or
Braeswood Conference Center 713 797 9000
if Marriott is full. They are not quite as close but have shuttles to the
Medical Center.

Houston's Hobby (HOU) airport is closest to the Medical Center. Shuttle
service (Airport Express) is available from Hobby or Intercontinental (IAH)
to Medical Center Hotels. Southwest serves Hobby & has some excellent
fares. IAH is a Continental hub.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From anna at aretha.jax.org Mon Jan 10 10:02:19 2000
From: anna at aretha.jax.org (Anna Anagnostopoulos)
Date: Wed Aug 20 11:55:23 2003
Subject: [Fwd: knockout poster]
Message-ID: <3879F47A.BCFD2293@aretha.jax.org>

Hello everyone,

Can anyone help Dr. Nottoli finding this poster? Please e-mail your
responses directly to timothy.nottoli@yale.edu

Thanks!

Anna
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An embedded message was scrubbed...
From: Timothy Nottoli <Timothy.Nottoli@yale.edu>
Subject: knockout poster
Date: Fri, 07 Jan 2000 16:03:06 -0500
Size: 2179
Url: http://mailman.ic.ac.uk/mailman/private/transgenic-list/attachments/20000110/f115dadc/attachment.eml
From anshin at hotmail.com Mon Jan 10 08:56:51 2000
From: anshin at hotmail.com (Anshin Lanugo)
Date: Wed Aug 20 11:55:23 2003
Subject: [Fwd: knockout poster]
Message-ID: <20000110135652.3534.qmail@hotmail.com>

Dear Tbase Group,
If anyone knows where to get this poster could they email me the info as
well?

Thanks!
anshin@hotmail.com

Tania Grunewald
University of Toronto
Genetics and Molecular Biology

>Subject: [Fwd: knockout poster]
>Date: Mon, 10 Jan 2000 10:02:19 -0500
>
>Hello everyone,
>
>Can anyone help Dr. Nottoli finding this poster? Please e-mail your
>responses directly to timothy.nottoli@yale.edu
>
>Thanks!
>
>Anna

>>Date: Fri, 07 Jan 2000 16:03:06 -0500

>>Dear Tbase folks:

>>I've noticed a poster around outlining the procedure for making a
>>knockout.� I've just started up in a knockout facility at Yale, and >>such
>>a poster would be handy for potential users to consult.� How >>do I get
>>hold of one?� Please let me know.� Thanks.

>>Sincerely,

>>Tim Nottoli
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From p.w.elbers at amc.uva.nl Mon Jan 10 16:48:30 2000
From: p.w.elbers at amc.uva.nl (Paul Elbers)
Date: Wed Aug 20 11:55:23 2003
Subject: Announcement: The 2nd International Amsterdam Mouse Symposium
Message-ID: <01BF5B8A.8736E740.p.w.elbers@amc.uva.nl>

Dear Colleagues,

On behalf of Prof. Dr. Ince from the dept. of experimental Anesthesiology
and physiology of the Academic Medical Center in Amsterdam, The Netherlands
I would like to draw the attention of all interested in transgenics to the

13-15 April 2000, under auspices of the International Society for Heart
Research

2nd International Amsterdam Mouse Symposium;
cardiovascular physiology of mice

http://www.usouthal.edu/ishr/Amst-Mouse.htm

Topics in this exciting conference include:
- Physiological research in mice
- Functional genomics and adaptive mechanisms in knockout and transgenic
mice
- Mouse models of cardiovascular disease
- Future directions: reporter genes, inducible genes and gene therapy

Furthermore there will be poster sessions and an industrial exhibition.
Fifty selected abstracts will be published in a special issue of Basic
Research in Cardiology.

Please visit our website for more information and registration
http://www.usouthal.edu/ishr/Amst-Mouse.htm

Yours Sincerely,

Paul Elbers, MD
organizing committee ' The 2nd International Amsterdam Mouse Symposium'

Academisch Medisch Centrum, Experimentele Anesthesiologie
M.0-010
Meibergdreef 9, 1105 AZ, Amsterdam
tel 5665242, fax 6979004, e-mail p.w.elbers@amc.uva.nl

*****
Come to our 2nd International Amsterdam Mouse Symposium: cardiovascular
physiology of mice
Check all the details on: http://www.usouthal.edu/ishr/Amst-Mouse.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Cynthia.Gander at stjude.org Mon Jan 10 10:34:08 2000
From: Cynthia.Gander at stjude.org (Gander, Cynthia)
Date: Wed Aug 20 11:55:23 2003
Subject: job opening
Message-ID: <83B4CF76D818D111982000805F31CA5302A3F4B0@sjmemexc1.stjude.org>

Dear Members,

Please read below. If you have any questions and/or interested in applying
please email Virgil Holder at virgil.holder@stjude.org
<mailto:virgil.holder@stjude.org> or see our web site at www.stjude.org
<http://www.stjude.org> .

Cryopreservation Supervisor, Animal Resources Center, PC# AR0028, Req# 2408
Job Summary: Establishes and directly supervises the operation of the ARC's
mouse embryo cryopreservation and rederivation service. Assists the
Department of Genetics in providing other transgenic services to staff and
faculty. Requirements: Bachelor's Degree in life science or a related area
required. Formal training in transgenic technology including
cryopreservation techniques required. Five years experience in the
transgenic animal field required including the cryopreservation of mouse
embryos. Supervisory experience required.

Thanks,

Cynthia
Cynthia Gander LATg
Breeding Colony Coordinator
Animal Resource Center
St. Jude Children's Research Hospital
332 N. Lauderdale
Memphis, TN 38105-2794
Phone: 901-495-3506
Fax: 901-495-3112
www.stjude.org <http://www.stjude.org>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From cware at u.washington.edu Mon Jan 10 08:45:57 2000
From: cware at u.washington.edu (C. Ware)
Date: Wed Aug 20 11:55:23 2003
Subject: [Fwd: knockout poster]
In-Reply-To: <3879F47A.BCFD2293@aretha.jax.org>
Message-ID: <Pine.A41.4.10.10001100845080.204720-100000@homer19.u.washington.edu>

The poster comes from Jackson Labs. I forget it's title, but it mentioned
in their most recent newsletter.

On Mon, 10 Jan 2000, Anna Anagnostopoulos wrote:

> Hello everyone,
>
> Can anyone help Dr. Nottoli finding this poster? Please e-mail your
> responses directly to timothy.nottoli@yale.edu
>
> Thanks!
>
> Anna
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From mmm at cnio.es Mon Jan 10 18:22:52 2000
From: mmm at cnio.es (Marcos.Malumbres)
Date: Wed Aug 20 11:55:23 2003
Subject: [Fwd: knockout poster]
Message-ID: <F0DF64776D4DD31181F400A0C9DB2829044A17@oncogen.cnio.es>

The title of the poster is: "Gene Targeting with 129 strains" and it's
distributed by The Jackson Laboratory. You can contact:

JAX Mice Orders and Technical Support
Tel. 800-422-MICE or 207-288-5845
Fax 207-288-6150
http://www.jax.org/jaxmice

________________________________________
Marcos Malumbres, Ph.D.

Centro Nacional de Investigaciones Oncol?gicas Carlos III
Centro Nacional de Biotecnolog?a, Lab. 118
Campus de Cantoblanco, E-28049 Madrid, Spain
Tel. 34-91-5854678; Fax 34-91-3720193
<marcos.malumbres@cnio.es>
_________________________________________

> ----------
> From: Anna Anagnostopoulos
> Reply To: Anna Anagnostopoulos
> Sent: lunes, 10 enero 2000 16:02
> To: transgenic-list@ic.ac.uk
> Subject: [Fwd: knockout poster]
>
> <<Message: knockout poster>>
> Hello everyone,
>
> Can anyone help Dr. Nottoli finding this poster? Please e-mail your
> responses directly to timothy.nottoli@yale.edu
>
> Thanks!
>
> Anna
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From janice_penney at hotmail.com Mon Jan 10 12:25:27 2000
From: janice_penney at hotmail.com (Janice Penney)
Date: Wed Aug 20 11:55:23 2003
Subject: mouse milking
Message-ID: <20000110172527.57012.qmail@hotmail.com>

Dear list

I am presently trying to milk mice on a fairly regular basis and I was
wondering if any of you have any tips you can pass my way. First of all I
have been anesthetizing the mice using a portable anasthetic machine
(isoflurane) then manually massaging the gland to expel the milk. I have
tried some set-ups with syringes and flexible tubing but have not found that
they work very well. I was getting 50uL per mouse but am now being told
that more than 50 is needed. My original intent was to become adept at the
milking so that I would no longer have to anasthetize the mice to get the
milk as the anasthetic machine is a borrowed one and the facility is now
insisting that I set up a scavenging system in order to use it.

My first question is: Does anyone know a relatively easy way for one person
to get over 50 uL of milk from 6 lactating mice in one day, without
anasthetizing them?

My second question is: For those of you using anasthetic machines, what sort
of scavenge system do you use? I am presently worhing in a sterile hood
(the air flows from the ceiling of the hood) and it is fairly small. I use
a rebreathing tube with a face mask that the mouse's whole head can fit in
to and I have placed a latex glove around the mask with a slit cut in it so
that the seal around the mouse's neck is pretty snug. Other than the filter
at the end of the tubing, I have no scavenge system. What should be added
here?

Please reply directly to me and I will send a summary to the list if people
are interested. Thanks very much for any suggestions.

Janice

Janice Penney

Transgenic Technician
827 McIntyre Medical Sciences Building
3655 Promenade Sir William Osler
McGill University
Montreal PQ
H3G 1Y6
Ph (514) 398-2956
FAX (514) 398-7384
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From fmargoli at umaryland.edu Mon Jan 10 14:22:46 2000
From: fmargoli at umaryland.edu (Frank Margolis)
Date: Wed Aug 20 11:55:23 2003
Subject: polycystic ovaries
Message-ID: <v04210114b49fdd9d234d@[134.192.145.156]>

>Dear Colleagues

>We have noticed that a line of KO mice on the 129SvImJ (JAX strain
>#2488) background have frequent "multifocal hemorrhagic enlargement
>of the uterus associated
with polycystic ovaries". This is especially true in older females.

Has this been a general observation in older mice of the 129 lines or
in other KO lines or a hint of dysfunction associated with our KO?

Any input appreciated

Frank

Frank L. Margolis Ph.D.
Professor
Department of Anatomy and Neurobiology
HSF280
University of Maryland School of Medicine
685 West Baltimore Street
Baltimore MD 21201

phone 410-706-8913/4
fax 410-706-2512
fmargoli@umaryland.edu
http://neurobiology.umaryland.edu/
http://neuroscience.umaryland.edu/
"Any sufficiently advanced technology is indistinguishable from
magic" Arthur C. Clarke

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From josepha at amgen.com Mon Jan 10 14:05:54 2000
From: josepha at amgen.com (Anderson, Joe)
Date: Wed Aug 20 11:55:23 2003
Subject: mouse milking
Message-ID: <401D3C121A10D3118A7F0008C7CF080E2C6ACC@tan-exch.amgen.com>

Hi Janet,

I have some info for you but your address at hotmail keeps bouncing the
message back to me with an "account inactive" error.

Do you have another address?

Joe Anderson
Josepha@amgen.com <mailto:Josepha@amgen.com>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From ccl at aretha.jax.org Tue Jan 11 13:21:22 2000
From: ccl at aretha.jax.org (Carol Cutler Linder)
Date: Wed Aug 20 11:55:23 2003
Subject: Gene Targeting with 129 strains
Message-ID: <v03007817b4a1311c1278@[209.194.200.19]>

Hi List,

Sorry I wasn't on the ball answering this question. Thanks Marcos for
supplying this information. The Jackson Laboratory is distributing a
complimentary wall chart that gives an overview of gene targeting, shows
the derivation of several ES cell lines, goes into the issues of coat color
genetics, and creating congenics and coisogenic strains of targeted mutant
mice. The information is based on our Nature Genetics article on 129
strains (Vol 16:19-27, 1997).

You can email me directly (ccl@jax.org) with a complete mailing address and
I will forward the request on to Customer Service or you can contact
Customer Service by phone.

Best regards,
Carol

Marcos Malumbres, Ph.D. wrote:
The title of the poster is: "Gene Targeting with 129 strains" and it's
distributed by The Jackson Laboratory. You can contact:

JAX Mice Orders and Technical Support
Tel. 800-422-MICE or 207-288-5845
Fax 207-288-6150
http://www.jax.org/jaxmice

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Carol Cutler Linder, Ph.D.
Senior Technical Information Scientist
Marketing Communications
The Jackson Laboratory
21 Country Club Dr.
Las Vegas, NM 87701-4609
Phone: (505) 425-5971
FAX: (505) 425-2880 E-mail: ccl@jax.org

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From lin_gan at urmc.rochester.edu Wed Jan 12 12:45:37 2000
From: lin_gan at urmc.rochester.edu (Lin Gan)
Date: Wed Aug 20 11:55:23 2003
Subject: C57BL6 ES cells
Message-ID: <v03110701b4a26d99b61b@[128.151.151.198]>

Dear List,

I am looking for a source for the C57BL6 ES cell lines. I would
appreciate your input and any experience/advice regarding the C57BL ES
cells.

Lin Gan, Ph.D.
Assistant Professor
Center for Aging and Developmental Biology
KMRB 1-9625
University of Rochester
601 Elmwood Avenue, Box 645
Rochester, New York 14642
Phone: (716)273-1510 (O)
(716)273-1512 (L)
FAX: (716)756-7665
e-mail: lin_gan@urmc.rochester.edu
gan_lin@hotmail.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From DJ_Kappes at fccc.edu Thu Jan 13 10:55:02 2000
From: DJ_Kappes at fccc.edu (Dr. Dietmar Kappes)
Date: Wed Aug 20 11:55:23 2003
Subject: Transgenic and knockout positions
Message-ID: <B4A35F86@arir.fccc.edu>

Fox Chase Cancer Center in Philadelphia has 2 job openings for individuals
skilled in transgenic and ES cell work. See descriptions below.

Manager of Embryonic Stem Cell Production Facility. The successful candidate
will be responsible for the start-up and day-to-day operation of an ES Cell
Culture Facility, which will generate ES lines with specific targeted mutations
for Fox Chase Cancer Center researchers. The applicant must have 3 or more
years of experience in the maintenance and manipulation of murine embryonic
stem (ES) cells, including transfection, selection and identification of germ-
line competent clones. The successful candidate will also be responsible for
advising investigators on experimental strategy and must possess an in-depth
understanding of targeting vector design. Salary commensurate with experience.

Manager of Transgenic Mouse Facility. The successful candidate will be
responsible for the day-to-day operation of a Transgenic Mouse Core Facility,
which will generate both transgenic and knockout mouse lines for Fox Chase
Cancer Center researchers. The applicant must have 5 or more years of
experience in all relevant procedures, including pronuclear and blastocyst
injection and embryo transfer. The successful candidate will be responsible for
supervising one or more technical specialists and for advising investigators on
experimental strategy. Salary commensurate with experience.

Fox Chase Cancer Center is world-renowned for excellence in basic and clinical
biomedical research. The basic sciences division comprises 40 programs
focussing on immunology, virology, structural biology, and cellular and
developmental biology. The center is located in a pleasant residential setting
and offers excellent benefits.

Direct inquiries to: Dr. Dietmar Kappes, Fox Chase Cancer Center, 7701 Burholme
Avenue, Philadelphia 19111; Tel.: 215-728-5374; E-mail: dj_kappes@fccc.edu.

--------------------------------------------------------------
Dr. Dietmar Kappes at:
DJ_Kappes@fccc.edu
Fox Chase Cancer Center
7701 Burholme Ave.
Philadelphia, Pa. 19111
Tel: (215) 728-5374
Fax: (215) 728-2412

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From kellek01 at doc.mssm.edu Thu Jan 13 12:20:11 2000
From: kellek01 at doc.mssm.edu (Kevin Kelley)
Date: Wed Aug 20 11:55:23 2003
Subject: culturing of C57Bl/6 eggs
Message-ID: <387E0D7B.1924@doc.mssm.edu>

Dear list,

We have been attempting to culture C57Bl/6 1-cell eggs to the
blastocyst stage without any success. A few may go as far as two-cell,
but no further. Eggs cultured from hybrid mice (B6C3 F1), on the
otherhand, will go to blastocyst without any problems under the same
conditions in the same incubator (KSOM media, 5% CO2).

Can anyone provide us with helpful hints on any changes that may be
necessary for Bl/6 egg culture conditions? I belive that this issue may
have been addressed in past discussions by users of the list, and if so
I apologize for the repetition.

Thank you,
Kevin A. Kelley, Ph.D.
Associate Director, Center for Laboratory Animal Sciences
Director, Transgenic Services

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From kmoore at animal.ufl.edu Thu Jan 13 14:33:36 2000
From: kmoore at animal.ufl.edu (Karen Moore)
Date: Wed Aug 20 11:55:23 2003
Subject: Position Available
Message-ID: <000701bf5dfd$160ca620$3304e30a@nagapc.dps.ufl.edu>

A Biologist position is currently available at the University of Florida
for a highly motivated individual interested in conducting research in the
fields of embryology, nuclear transfer, transgenics and reproductive
physiology. Primary responsibilities will include molecular biology
techniques, embryo and tissue culture, micromanipulation, animal husbandry,
record keeping and general laboratory management. Experience in one or more
of these areas is desired but not required. The successful candidate will
have a B.S. or M.S. degree in biological sciences or a related disipline, be
well organized and enthusiastic, and be able to interact well with others.
Salary will be commensurate with qualifications and experience. Interested
applicants should contact me directly for information pertaining to the
application procedure at the email or phone listed below:

Karen Moore, Ph.D.
University of Florida
Department of Dairy and Poultry Sciences
PO Box 110920
Gainesville, FL 32611-0920
(352)392-1958
(352)392-5595 fax
email: kmoore@animal.ufl.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From RobinsoM at pediatrics.ohio-state.edu Thu Jan 13 14:25:00 2000
From: RobinsoM at pediatrics.ohio-state.edu (Robinson, Michael)
Date: Wed Aug 20 11:55:23 2003
Subject: BAC transgenics
Message-ID: <1366FE962DB3D311B8DA00A0C9EA0484099E96@RESMS01.net.chi.ohio-state.edu>

Dear List,

We are currently having difficulty making transgenic mice by injecting
bacterial artificial chromosomes (BACs). We would really like to be able to
develop this technique in the near future and to do so, I think that it
would be very helpful to have in hand a BAC that would produce an obvious
embryonic or neonatal phenotype that would make it easy to determine our
success without the need to perform DNA analysis. The obvious candidate
that comes to mind is the tyrosinase gene. This gene is about 80,000 bp and
therefore expression would only be expected if a large intact fragment of
DNA were integrated into the genome.

Does anyone know of BAC clones that have been identified to carry an intact
functional copy of the tyrosinase gene? Alternatively, does anyone have any
other BACs containing a gene that could easily distinguish expressing
transgenic mice?

I sincerely appreciate any comments and/or advice about this matter.

Mike Robinson, Ph.D.
Division of Molecular and Human Genetics
Children's Research Institute
700 Children's Drive
Columbus, OH 43205
Phone 614-722-2764
FAX: 614-722-2716

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From nagy at mshri.on.ca Thu Jan 13 17:48:47 2000
From: nagy at mshri.on.ca (Andras Nagy)
Date: Wed Aug 20 11:55:23 2003
Subject: HPRT minus R1
Message-ID: <387E483D.A6ECFB31@mshri.on.ca>

Hi All,

I am wondering if anyone knows about the existence of HPRT deficient R1
ES cells. We are planning to derive one but my feelings say that it
might already exist somewhere, since we sent out a few hundred vials of
R1 already since it was established in 1991.
Thanks for the info and may be - eventually - for the cells.

Andras Nagy PhD
Senior Staff Scientist
Mount Sinai Hospital
Samuel Lunenfeld Research Institute
600 University Ave.
Toronto, M5G 1X5
Canada

tel. (416)-586-3246 0ffice
tel. (416)-586-8455 Lab
tel. (416)-586-5361 Secretary
fax (416)-586-8588
URL: http://www.mshri.on.ca/nagy/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From Fred.Sablitzky at nottingham.ac.uk Fri Jan 14 10:50:12 2000
From: Fred.Sablitzky at nottingham.ac.uk (Fred Sablitzky)
Date: Wed Aug 20 11:55:23 2003
Subject: position available
Message-ID: <211D16E31A5@pdn1.gene.nottingham.ac.uk>

Post-doctoral position available

Applications are invited for a post-doctoral research position funded by the EU 5th
Framework Program for up to three years to investigate the functional role of Id proteins in
haematopoiesis and cancer. We developed mouse mutants carrying a dormant Id4 transgene
gene which can be activated in a lineage specific way via Cre-mediated recombination. The
highly motivated candidate should have experience in molecular biology, pronuclear
injection, embryonic stem cells and haematopoiesis.
The position is available from the 1 Feb. 2000. Salary will be on the RAIA scale.
Please send your CV and names and address of two references to

fred.sablitzky@nottingham.ac.uk

For more information see:

http://www.ifz.uni-essen.de/eu/Inhaltsverzeichnis.html

http://www.nottingham.ac.uk/genetics/Fred%20Sablitzky.htm

---- NEW address ----

Prof. Fred Sablitzky
The University of Nottingham
Institute of Genetics
Queen's Medical Centre
Nottingham
NG7 2UH

phone: 0115 849 3242
fax: 0115 849 3243
email: fred.sablitzky@nottingham.ac.uk
http://www.nottingham.ac.uk/genetics/Fred%20Sablitzky.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From nagy at mshri.on.ca Fri Jan 14 11:21:21 2000
From: nagy at mshri.on.ca (Andras Nagy)
Date: Wed Aug 20 11:55:23 2003
Subject: Cre recombinase database
Message-ID: <387F3ECE.EBAC775D@mshri.on.ca>

Dear All,

As far as my current knowledge is concerned about the releasable
information I updated the Cre recombinase data base homed on my lab web
page <http://www.mshri.on.ca/nagy/>. I also included those which are
going to be published in the next month issue of "genesis".

I appreciate any help in keeping this data base absolutely updated. If
you find anything missing, for example, lines which are made, the
information is releasable, please let me know.

Thank,

Andras
nagy@mshri.on.ca

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From DonovanD at grc.nia.nih.gov Fri Jan 14 16:40:05 2000
From: DonovanD at grc.nia.nih.gov (Donovan, Dave)
Date: Wed Aug 20 11:55:23 2003
Subject: dirty facility cleanup
Message-ID: <C5BE6D142223D211909E0000F8CD228A014AC4B0@c3.grc.nia.nih.gov>

Dear List,

Our animal facility has run into a parvovirus infection, and I was hoping to
talk to other Facility Managers/Vets who have had to deal with this kind of
problem. If you have made either successful or unsuccessful attempts to clean
up a facility, I would appreciate hearing from you about both, so that we do not
reinvent the wheel or end up having to relive this nightmare, due to a failed
attempt.

Our main concern: How do we keep the dirty mice breeding so that the PIs can
still perform experiments while we are cleaning up the facility? May we phase
out the dirty and gradually replace dirty with clean, or do we need to make a
clean break, remove all mice before even attempting a clean up? (If you agree
that we can successfully phase out dirty and gradually replace with clean, what
are the "golden rules" that we should follow during this process, eg. should all
dirty mice be housed on a separate room/corridor/floor and clean mice be housed
in another?)

Please respond and let me know what you have tried.... what has failed.... and
what has worked. If you feel your answer would demand too much detail to
readily answer on line, please call and I will attempt a summary for the list.
In any event, please include a phone number so I can call you back and ask your
advice on some specific questions related to our situation.

Thanks in advance.

Dave

David M. Donovan, Ph.D.
Chief, Transgenic and Knockout Facility Section
Gerontology Research Center, IRP, NIA, NIH
5600 Nathan Shock Drive
Baltimore, MD 21224-6825
410-558-8111 voice
410-558-8236 fax

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From sfk at bilbo.bio.purdue.edu Mon Jan 17 09:28:42 2000
From: sfk at bilbo.bio.purdue.edu (Steve Konieczny)
Date: Wed Aug 20 11:55:23 2003
Subject: Director, Transgenic Facility Position
Message-ID: <4.1.20000117090436.00a0bc60@bilbo.bio.purdue.edu>

Dear Colleagues,

The Purdue University NCI-designated Cancer Center has an immediate opening for
an individual to assume operational responsibility for the newly expanded
Purdue Cancer Center Transgenic Mouse Core Facility (TMCF). Below you will
find the advertisement that will be appearing in several issues of Science
within the next few weeks. The TMCF enjoys broad financial support from both
the NCI as well as Purdue University with guaranteed recurring funds to support
this endeavor. This is an ideal position for an individual who would like to
take on the challenges of overseeing this newly expanded Facility. The TMCF
offers both transgenic and knock-out services to the Purdue community as well
as to other investigators in the state. We are looking for someone who has
knowledge and experience in generating both model systems as well someone who
enjoys being able to completely oversee the many duties that are associated
with running this facility.

Please be sure to circulate this notice to any individual or laboratory that
you might think would be interested in this position. If there are specific
questions feel free to contact me directly at the address below. Thank you for
taking the time to read this notice and for helping us to find a qualified
individual.

Sincerely,

Stephen F. Konieczny, Professor
Department of Biological Sciences
Purdue University
West Lafayette, IN 47907-1392

Tel: 765-494-7976
Fax: 765-496-2536
email: sfk@bilbo.bio.purdue.edu

____________________________________________________________________________
____________

Research Scientist, Transgenic Mouse Core Facility
Purdue University Cancer Center

The NCI-designated Cancer Center at Purdue University has an immediate opening
for a qualified individual to assume operational responsibility for the Cancer
Center's newly expanded and fully operational Transgenic Mouse Core Facility
(TMCF). The TMCF offers both knockout and conventional transgenic services to
the Purdue University community. Responsibilities and duties for this position
include the generation of transgenic mice by pronuclear injection, gene
targeting in ES cells, the derivation of chimeric mice plus supervision of all
support staff. The qualified candidate will have a M.S. or Ph.D. degree (Ph.D.
preferred), experience in both ES cell culture and transgenic mouse procedures
plus excellent communication and interpersonal skills. Applicants should send
their curriculum vitae and the names of at least three references to: TMCF
Position, Purdue Cancer Center, Hansen Life Sciences Research Building, Purdue
University, West Lafayette, IN 47907-1524. See Websites:
http://www.pharmacy.purdue.edu/~ccenter/ or
http://www.bio.purdue.edu/courses/Koniecznylab/intropage.html for more
information.

Purdue University is an Equal Opportunity/Affirmative Action Employer.

____________________________________________________________________________
____________
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From sauerb at omrf.ouhsc.edu Tue Jan 18 00:25:07 2000
From: sauerb at omrf.ouhsc.edu (Brian Sauer)
Date: Wed Aug 20 11:55:23 2003
Subject: VIVARIUM DIRECTOR Position Opening
Message-ID: <v0311070cb4a90ad49092@[157.142.201.104]>

Dear Colleagues,

The Oklahoma Medical Research Foundation has an immediate opening for
Vivarium Director. Below is the advertisement describing this position, and
which will be appearing in a number of scientific journals shortly. This is
really a great opportunity -- inquiries should be addressed to Dr. Morris
Reichlin, as described below.

Brian Sauer, Ph.D.
Member, Oklahoma Medical Research Foundation
Head, Developmental Biology Program
Director, Microinjection Core Facility
Adjunct Professor of Cell Biology, Univ. Oklahoma Health Sciences Center

************************************************************
VIVARIUM DIRECTOR, DVM

The Oklahoma Medical Research Foundation (OMRF) invites applications for the
position of Director of Laboratory Animal Resource Center to manage and
direct the OMRF animal services, while maintaining compliance with all
regulations and actively interacting with the scientific staff. The
Laboratory Animal Resource Center (LARC) at OMRF provides rodent animal care
services to OMRF researchers and other researchers within the Oklahoma
Health Sciences Center of the University of Oklahoma, which is adjacent to
OMRF. Currently OMRF's animal facility is AAALAC accredited and provides
support for the only Transgenic and Knockout Gene Mouse Facility in the
state of Oklahoma.

The current 10,000 square foot LARC was completely renovated in 1998 and
will be complemented by a new, separate, state-of-the-art 10,500 square foot
barrier mouse facility that will begin construction in the Year 2000.

The Oklahoma Medical Research Foundation (OMRF) located in Oklahoma City is
one of the nations leading independent biomedical research institutions.
OMRF prides itself on its scientific excellence that is supported by a
simple collegial organizational structure that supports our biomedical
research mission. OMRF employs approximately 400 employees, of which 40 are
principal investigators, including two Howard Hughes Medical Institute
supported researchers. OMRF spends approximately $27 million per year.
Approximately 50% of the operating budget is made up of competitive grants,
mostly from the NIH.

The Vivarium Director will report to the Vice President of Research and will
have operational responsibility related to all of the animal research
facilities. Successful candidates will be ACLAM eligible, possess a D.V.M.
degree, an active state veterinary license, and have some previous
experience with transgenic animal colony management. Please send your
curriculum vitae with cover letter and three references along with salary
requirements to Dr. Morris Reichlin, Vice President of Research, Oklahoma
Medical Research Foundation, 825 N.E. 13th Street, Oklahoma City, OK 73104,
or by fax to (405) 271-4110. Additional information is available on the web
site www.omrf.ouhsc.edu . OMRF is an AA/EOE.
************************************************************

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Johannes.Wilbertz at cmb.ki.se Tue Jan 18 08:43:09 2000
From: Johannes.Wilbertz at cmb.ki.se (Johannes Wilbertz , Genetik)
Date: Wed Aug 20 11:55:24 2003
Subject: Trans-Tech-Meeting Stockholm
Message-ID: <l03110701b4a9c89fc52f@[130.237.120.195]>

Dear Colleagues,

below you can see the preliminary schedule for the Trans-Tech Meeting in
Stockholm.
If someone needs further information, just visit our homepage
(http://www.mousecamp.ki.se).

Regards

Johannes

===================================================

Trans-Tech-Meeting 2000
Stockholm

May 17th, 18th and 19th
2000

Organizers:
Cambridge University / Karolinska Institute

Preliminary Schedule

TALKS

Session: Transgenic mice; Genetic I
a) The origin of the lab mouse
b) New strains of mice (inbred / outbred) and their use in tg
production
c) Influence of the genetic background on experiments
d) Production of congenic mice / speed congenics

Session: Knockout mice and inducible systems; Genetic II
a) Different lines of ES cells
b) Inducible knockout systems
c) Reporter genes (LacZ, tau/lacZ, GFP etc.)
c) Induced mutagenesis in mice

Session: New techniques in the production of transgenic animals
a) Sperm mediated gene transfer
b) Cloning techniques
c) Tetraploids and aggregation

Session: Production of non-rodent transgenic animals and their use in
biomedical research
a) TG-Chicken Methods and use
b) TG-Fish Strains, methods and use
c) TG-Cattle Strains, methods and use

Session: Transgenic rodents in biomedical research
a) Immunology
b) Hypertension research (Rats)
c) Genetic
d) Pharmacology
e) Cancer research

Session: Animals and environment
a) Measuring of the behavior of tg-rodents (rotarod, water maze etc.)
b) Measuring of physiological parameters in the mouse
c) Influence of the environment on experiments
d) Food for tg-rodents (something to consider)

Session: Transgenic animals and health
a) Influence of infections on experiments
b) Germ-free transgenic animals (Production and use in biomedical
research)
c) EMMA (exchange and storage of transgenic lines)

Session: Safety management and animal facilities
a) Running a facility (conventional open unit or strict barrier
conditions)
b) IVC racks (a method to keep valuable animals free of infections)
c) Marking of animals (ear tags, transponder systems, available
databases etc.)

POSTER PRESENTATIONS

a) Latest findings in transgenic technology
b) Interesting models in biomedical research (rodents and non-rodents)
c) New developments in animal husbandry

=====================================

Johannes Wilbertz, Ph.D.
Head of Campus Transgene Facility
Karolinska Institute
CMB / MouseCamp
Von Eulers v?g 3
171 77 Stockholm
Sweden

Tel. Office: + 46 8 728 7318
Tel. Inj.lab.: + 46 8 728 7613
Tel. mobile: + 46 70 4714985
Fax: + 46 8 308374
E-Mail: johannes.wilbertz@cmb.ki.se

http://www.mousecamp.ki.se/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From chenm0 at uthscsa.edu Tue Jan 18 10:57:33 2000
From: chenm0 at uthscsa.edu (chenm0)
Date: Wed Aug 20 11:55:24 2003
Subject: job wanted
Message-ID: <01JKUIVCPC1GA3ETS3@uthscsa.edu>

A person who has educational tranining in Animal Husbandry (BS), Animal
Genetics (MS) and is a candidate for his second MS in Cancer Biology. His
professional work experience during the last ten years included quality
control of laboratory animals & animal experiments; generation of
transgenic/knockout mice; gene trap strategy; antibody production; Production
of recombinant proteins; site-directed mutagenesis; generation of stable
inducible cell lines. He is well informed both in animal science and as well
as cell and molecular biology. He seeks a position either as a manager of a
tansgenic facility or as a research scientist/senior research associate in an
area which requires both animal science and cell/molecular biology background.
Contact information for the above applicant: Michael may626@yahoo.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Matthias.Ernst at ludwig.edu.au Wed Jan 19 10:55:57 2000
From: Matthias.Ernst at ludwig.edu.au (Matthias Ernst)
Date: Wed Aug 20 11:55:24 2003
Subject: design of transgene construct
Message-ID: <805AD25D3702D311805900062B004E420F4E41@keeper.ludwig.edu.au>

Dear List

Our lab is interested in developing a system for driving tissue-specific
expression of cDNAs in transgenic mice using a promoter that we have
characterised in vitro. We would like to design a construct containing a
floxed beta-galactosidase reporter upstream of a cDNA of interest (5'-
promoter-lox(P)-bgal-pA-lox(P)-cDNA-pA). In this way, we will be able to
ascertain (i) the spatio-temporal activity of our promoter fragment in
vivo; and (ii) to drive expression of a cDNA of interest following
Cre-mediated excision of the b-gal reporter gene in vivo.
There are three question relating to the design of this construct:
1) What is the optimal positioning of the 5' lox(P) sequence with
respect to the ATG initiation codon/Kozak sequence of b-gal and the
transcriptional start site in our promoter?
2) What is the current thinking on the introduction of introns into
transgenic constructs and where should they be positioned in a
"bifunctional" transgenic construct?
3) What is the current view regarding the benefit of using insulator
sequences on either end of transgenes in order to minimize positional
integration effects?

Matthias Ernst

Matthias Ernst, Ph.D
Joint Laboratory Head
Colon Molecular and Cell Biology
Ludwig Institute for Cancer Research
PO Royal Melbourne Hospital, VIC 3050
AUSTRALIA
Phone: (+61)-3-9341-3155/3149
Fax: (+61)-3-9341-3191
e-mail matthias.ermst@ludwig.edu.au

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From DonovanD at grc.nia.nih.gov Wed Jan 19 08:49:39 2000
From: DonovanD at grc.nia.nih.gov (Donovan, Dave)
Date: Wed Aug 20 11:55:24 2003
Subject: design of transgene construct
Message-ID: <C5BE6D142223D211909E0000F8CD228A014AC4CC@c3.grc.nia.nih.gov>

Dear Matthias,

I strongly recommend that you first make a simple construct of your promoter
fused to an easily assayable reporter (lac Z). Promoter studies "in vitro" (I
am assuming you mean a cell culture system) yield notoriously different
expression patterns than whole animal (transgenic) systems.
I suggest you start off with the longest promoter fragment you can readily get
your hands on.

Dave
David M. Donovan, Ph.D.
Chief, Transgenic and Knockout Facility Section
Gerontology Research Center, IRP, NIA, NIH
5600 Nathan Shock Drive
Baltimore, MD 21224-6825
410-558-8111 voice
410-558-8236 fax

-----Original Message-----
From: Matthias Ernst [mailto:Matthias.Ernst@ludwig.edu.au]
Sent: Tuesday, January 18, 2000 6:56 PM
To: 'transgenic-list@ic.ac.uk'
Subject: Re: design of transgene construct

Dear List

Matthias Ernst

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From garofalo at ermes.cba.unige.it Wed Jan 19 16:26:05 2000
From: garofalo at ermes.cba.unige.it (Silvio Garofalo)
Date: Wed Aug 20 11:55:24 2003
Subject: Nikon/Narishige IM-88 injectors
Message-ID: <l03130301b4ab85759d9d@[130.251.106.105]>

Can anybody advice about the Nikon/Narishige IM-88 microinjectors for
pronuclei injection
(http://www.nikon.co.jp/inst/Biomedical/nt88ne/index.html)? Is anybody
using them?

Thanks a lot

===================================================================
Silvio Garofalo, MD/PhD
Istituto Nazionale per la Ricerca Sul Cancro
Centro Biotecnologie Avanzate
Largo Rosanna Benzi n. 10
16132 Genova
Italia

Tel.: +39 010 5737 393
Fax: +39 010 5737 405
e-mail: garofalo@ermes.cba.unige.it
===================================================================

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From DJ_Kappes at fccc.edu Wed Jan 19 11:44:52 2000
From: DJ_Kappes at fccc.edu (Dr. Dietmar Kappes)
Date: Wed Aug 20 11:55:24 2003
Subject: No subject
Message-ID: <B4AB5434@arir.fccc.edu>

Postdoctoral position available immediately to undertake physical mapping of a
mutation responsible for murine CD4 T cell deficiency. Phenotypic analysis
indicates that the HD gene is of major importance in the decision-making
process that gives rise to the alternate CD4+ helper and CD8+ killer T cell
lineages (Keefe et al, Science, 286, 1149-53, 1999). We have localized the
defect by genetic means to a 3cM interval, for which partial YAC and BAC
coverage exists. Expertise in molecular biology and particularly physical
mapping approaches is essential. NIH funded position: US citizenship or
permanent residency required. Send a resume to: Dietmar Kappes, Fox Chase
Cancer Center, Philadelpia (e-mail: dj_kappes@fccc.edu).

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Paul.Booth at agrsci.dk Wed Jan 19 17:56:55 2000
From: Paul.Booth at agrsci.dk (Paul Booth)
Date: Wed Aug 20 11:55:24 2003
Subject: No subject
Message-ID: <5166A14C6887D111991100805F8BB74903A76A8C@foulum01.agrsci.dk>

From: Paul.Booth@agrsci.dk
Subject: Dishes for morulae aggregation

Dear all,
Last November there was some discussion on the tg-list about dishes for
embryo aggregation. These comprise normal culture dishes having small holes
dug in them that are slightly larger than the size of an embryo. It was
suggested at that time by Dr Khillan that these dishes could in fact be
purchased through Genome Systems Inc. However, Genome Systems say that they
do not supply them any more and cannot recall which company originally made
them. Hence, I would greatly appreciate information from tg-list members
regarding the name of the manufacturer of these dishes.
Thank you in advance.
Paul
Paul Booth,
Dept. Animal Breeding and Genetics,
Section for Reproductive Biology,
Danish Institute of Agricultural Sciences,
Foulum, DK-8830,
Denmark.

Email: Paul.Booth@agrsci.dk
Tel: (45) 89 99 12 64
Fax: (45) 89 99 13 00

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From cfillat at supermac.iro.es Wed Jan 19 18:08:26 2000
From: cfillat at supermac.iro.es (fistro)
Date: Wed Aug 20 11:55:24 2003
Subject: Thy-1.2 promoter
Message-ID: <1263817187-26396266@supermac.iro.es>

Dear list,

I am interested in the generation of transgenic mice in which the
transgene will be specifically expressed in neurons. I was considering
the possibility of using the Thy-1.2 promoter. I have two questions
related to this issue.

1)Does any of you has had any experiemce with transgenic mice carrying
this promoter?
2)Does any of you has this prmoter available? or, do you konw who I could
ask for it?

Thank you very much,

Happy 2000!!

Cristina Fillat

Cristina Fillat
Centre de Genetica Medica i Molecular-IRO.
Hospital Duran i Reynals
Autovia de Castelldefels Km.2,7
08907-L'Hospitalet de Llobregat, Barcelona
Spain
Tel. 34 93 2607775
Fax. 34 93 2607776
e-mail:cfillat@iro.es

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From foleyk at zgi.com Wed Jan 19 10:27:57 2000
From: foleyk at zgi.com (Kevin Foley)
Date: Wed Aug 20 11:55:24 2003
Subject: FW: Thy-1.2 promoter
Message-ID: <200001191823.KAA1029526@stella.zgi.com>

You might also consider the neuron-specific enolase (NSE) promoter, which is
expressed in all differentiated neurons. It has been employed by many
groups in transgenic mice.

Cheers,
Kevin

Kevin P. Foley, Ph.D.
Scientist, Dept. of Genetics
ZymoGenetics, Inc.
1201 Eastlake Avenue East
Seattle, WA 98102
tel: (206) 442-6625
fax: (206) 442-6699
e-mail: foleyk@zgi.com

----------
>From: fistro <cfillat@supermac.iro.es>
>To: <transgenic-list@ic.ac.uk>
>Subject: Thy-1.2 promoter
>Date: Wed, Jan 19, 2000, 12:08 PM
>

>
> Dear list,
>
> I am interested in the generation of transgenic mice in which the
> transgene will be specifically expressed in neurons. I was considering
> the possibility of using the Thy-1.2 promoter. I have two questions
> related to this issue.
>
> 1)Does any of you has had any experiemce with transgenic mice carrying
> this promoter?
> 2)Does any of you has this prmoter available? or, do you konw who I could
> ask for it?
>
> Thank you very much,
>
> Happy 2000!!
>
> Cristina Fillat
>
> Cristina Fillat
> Centre de Genetica Medica i Molecular-IRO.
> Hospital Duran i Reynals
> Autovia de Castelldefels Km.2,7
> 08907-L'Hospitalet de Llobregat, Barcelona
> Spain
> Tel. 34 93 2607775
> Fax. 34 93 2607776
> e-mail:cfillat@iro.es
>
>
> "transgenic-list" web site (archives, FAQs, protocols, links) address:
> http://www.med.ic.ac.uk/db/dbbm/tglist.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From damak at inka.mssm.edu Wed Jan 19 13:57:30 2000
From: damak at inka.mssm.edu (Sami Damak)
Date: Wed Aug 20 11:55:24 2003
Subject: Cre mice
Message-ID: <3.0.6.32.20000119135730.007b9690@inka.mssm.edu>

Do you know a source, commercial or not, of Cre mice that can be used for
germ line removal of LoxP-flanked sequences.
Thanks

Sami Damak MD PhD
Research Assistant Professor
Department of Physiology and Biophysics
The Mount Sinai School of Medicine
1 Gustave L. Levy Place
New York NY 10029
Tel: 212-659 8694
Fax: 212-849 2599

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From nstwerra at yahoo.com Wed Jan 19 11:47:20 2000
From: nstwerra at yahoo.com (Kay-Uwe und Ulrike Wagner)
Date: Wed Aug 20 11:55:24 2003
Subject: Cre mice
Message-ID: <20000119194720.23835.qmail@web112.yahoomail.com>

Dear Sami,
Several Cre lines can do that:
1. EIIa-Cre mice (source: Heiner Westphal's group at
NIH), these mice are perfect for partial recombination
events, i.e. removal of neo cassette after 3-loxP
targeting strategy.
2. Zp3-Cre mice (I guess G. Martin's group in CA)
3. MMTV-Cre mice (Line A), 100% female germline
recombination, always complete recombination - no
partial recombination observed, I gave those mice to
the Jax Lab. They distribute this MMTV-Cre line using
this code:
129Bl6(TgN-MMTV-Cre)Mam1

I bet there are many more lines out there that could
be used for this purpose.

Kay

Kay-Uwe Wagner
NIH, NIDDK, LGP
Bethesda
KUW@nih.gov
--- Sami Damak <damak@inka.mssm.edu> wrote:
> Do you know a source, commercial or not, of Cre mice
> that can be used for
> germ line removal of LoxP-flanked sequences.
> Thanks
>
> Sami Damak MD PhD
> Research Assistant Professor
> Department of Physiology and Biophysics
> The Mount Sinai School of Medicine
> 1 Gustave L. Levy Place
> New York NY 10029
> Tel: 212-659 8694
> Fax: 212-849 2599
>
> "transgenic-list" web site (archives, FAQs,
> protocols, links) address:
> http://www.med.ic.ac.uk/db/dbbm/tglist.htm
>
>
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tsaunder at umich.edu Wed Jan 19 20:07:35 2000
From: tsaunder at umich.edu (Thom Saunders)
Date: Wed Aug 20 11:55:24 2003
Subject: Nikon/Narishige IM-88 injectors
In-Reply-To: <l03130301b4ab85759d9d@[130.251.106.105]>
Message-ID: <182984.3157301255@pm471-00.dialip.mich.net>

The equipment that we use for pronuclear microinjection is essentially the
same as that pictured in the URL you gave. The URL pictures a Nikon Eclipse
scope loaded down with every micromanipulator that Narishige makes. It's
overkill, you don't need all those additions. I use one hydraulic
micromanipulator (MO-188NE) to guide the injection needle. This
micromaipulator head needs to be mounted on a manual coarse
micromanipulator (MN-188NE). To position the holding pipet you need only
the motorized micromanipulator MM-188NE Also, I suggest that you use a
pneumatic microinjector instead of the IM-88 or IM-88H to control the
delivery of DNA. I prefer the one manufactured by Tritech, although
Nikon/Narishige also makes one. I prefer the Eppendorf (
http://www.eppendorfsi.com/ ) Cell-Tram Air to control the suction in the
holding pipet and the Eppendorf Cell-Tram Oil to control the aspiration and
ejection of ES cells during blastocyst microinjection. Eppendorf also sells
a very good alternative to the Narishige micromanipulators that you should
try out, the Transferman NK, you will need two of these, one for the
holding pipet and one for the microinjection needle.
Remember to get D.I.C. optics for the microscope (10X and 40X objectives),
you will need them to focus on the pronuclear membrane during
microinjection. If you are going to use the microscope for blastocyst
microinjection then you should also get a 20X D.I.C. objective.

Sincerely,

Thom

Thom Saunders, Ph.D.
Transgenic Animal Model Core
University of Michigan Medical School
Ann Arbor, MI 48109

http://www.med.umich.edu/tamc
tsaunder@umich.edu

--On Wed, Jan 19, 2000 4:26 PM +0100 Silvio Garofalo
<garofalo@ermes.cba.unige.it> wrote:

> Can anybody advice about the Nikon/Narishige IM-88 microinjectors for
> pronuclei injection
> (http://www.nikon.co.jp/inst/Biomedical/nt88ne/index.html)? Is anybody
> using them?
>
> Thanks a lot
>
> ===================================================================
> Silvio Garofalo, MD/PhD
> Istituto Nazionale per la Ricerca Sul Cancro
> Centro Biotecnologie Avanzate
> Largo Rosanna Benzi n. 10
> 16132 Genova
> Italia
>
> Tel.: +39 010 5737 393
> Fax: +39 010 5737 405
> e-mail: garofalo@ermes.cba.unige.it
> ===================================================================
>
>
>
> "transgenic-list" web site (archives, FAQs, protocols, links) address:
> http://www.med.ic.ac.uk/db/dbbm/tglist.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From murg at fas.harvard.edu Wed Jan 19 20:42:38 2000
From: murg at fas.harvard.edu (Stephanie Murg)
Date: Wed Aug 20 11:55:24 2003
Subject: Thy-1.2 promoter
In-Reply-To: <1263817187-26396266@supermac.iro.es>
Message-ID: <001c01bf62e7$a1dc4cc0$5f9bf78c@leverett.fas.harvard.edu>

Dear Cristina:
I am only familiar with Thy-1.2 as a CNS cell-surface glycoprotein (and its
use in marking donor septohippocampal transplant tissue), not as a promoter
per se. Like Kevin, I would also recommend the neuron-specific enolase (NSE)
promoter. Below are some references that you may find helpful:

Transcriptional activity of the neuron-specific enolase (NSE) promoter in
murine embryonic stem (ES) cells and preimplantation embryos.
Alouani S, Ketchum S, Rambosson C, Eistetter HR
Eur J Cell Biol 1993 Dec 62:2 324-32

Transgenic mice expressing beta-galactosidase in mature neurons under
neuron-specific enolase promoter control.
Forss-Petter S, Danielson PE, Catsicas S, Battenberg E, Price J, Nerenberg
M, Sutcliffe JG
Neuron 1990 Aug 5:2 187-97

*An inducible transgenic system using NSE (using a modified tTa approach):

Transgenic animals with inducible, targeted gene expression in brain.
Chen J, Kelz MB, Zeng G, Sakai N, Steffen C, Shockett PE, Picciotto MR,
Duman RS, Nestler EJ
Mol Pharmacol 1998 Sep 54:3 495-503

Stephanie Murg

____________________
Stephanie Murg
Harvard University
FAX 815.425.1732
TEL 617.493.2802
murg@fas.harvard.edu

> -----Original Message-----
> From: owner-transgenic-list@ic.ac.uk
> [mailto:owner-transgenic-list@ic.ac.uk]On Behalf Of fistro
> Sent: Wednesday, January 19, 2000 2:08 PM
> To: transgenic-list@ic.ac.uk
> Subject: Thy-1.2 promoter
>
>
>
> Dear list,
>
> I am interested in the generation of transgenic mice in which the
> transgene will be specifically expressed in neurons. I was considering
> the possibility of using the Thy-1.2 promoter. I have two questions
> related to this issue.
>
> 1)Does any of you has had any experiemce with transgenic mice carrying
> this promoter?
> 2)Does any of you has this prmoter available? or, do you konw who I could
> ask for it?
>
> Thank you very much,
>
> Happy 2000!!
>
> Cristina Fillat

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From clandel at jax.org Thu Jan 20 12:16:58 2000
From: clandel at jax.org (Carlisle Landel)
Date: Wed Aug 20 11:55:24 2003
Subject: Thy-1.2 promoter
In-Reply-To: <1263817187-26396266@supermac.iro.es>
References: <1263817187-26396266@supermac.iro.es>
Message-ID: <v04210106b4acf262c444@[199.94.155.139]>

At 12:28 PM -0500 1/19/00, fistro wrote:
>Dear list,
>
>I am interested in the generation of transgenic mice in which the
>transgene will be specifically expressed in neurons. I was considering
>the possibility of using the Thy-1.2 promoter. I have two questions
>related to this issue.

Christina,

You realize, of course, that Thy-1.2 will also go to T cells, right?

I spent some time trying to use Thy-1 as a T-cell directing
transgenic promoter. I'd recommend that you stay away from it. It
apparently has control elements scattered all through the gene.

Regards,

Carlisle Landel

PS: You can check out the following for Thy-1 transgenics:

Chen, S., Botteri, F., van der Putten, H., Landel, C.P., and Evans,
G.A. (1987) A lymphoproliferative abnormality associated with
inappropriate expression of the Thy-1 antigen in transgenic mice.
Cell 51: 7-19.

***********************************************************************
Carlisle P. Landel, Ph.D.
Cryopreservation Laboratory
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
"Cold? It's not cold, just a little chilly."
***********************************************************************
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From JOHN.MURASKI at ROCHE.COM Thu Jan 20 16:47:11 2000
From: JOHN.MURASKI at ROCHE.COM (Muraski, John {Geno~Palo Alto})
Date: Wed Aug 20 11:55:24 2003
Subject: Success of 129
Message-ID: <418F66121D9DD311A54B0000F80197880F6840@rplmsem1.pal.roche.com>

> Our group is debating backcrossing some chimeras developed from an E14
> cell line to the 129/Ola HSD strain. Before we do, we have a couple of
> issues to resolve hopefully fellow listmembers can resolve.
>
> We have heard that they are VERY poor breeders, even worse than the SvJ
> substrain. Is this true, and if so, how true (hopefully backed up with
> some data)?
>
> Does anyone have any experience with the breeding efficiency of the
> 129/Ola strain both as a congenic backcross strain, and as a production
> colony in general?
>
> Does anyone have any production statistics regarding this particular 129
> substrain versus other 129 substrains in particular the SvJ substrain?
>
> Thank you for all of your help with this dilemma. I will post a summary
> of all responses gathered on and off line to the list for others who may
> be interested in the results.
>
> John Muraski
> Roche Bioscience
> john.muraski@roche.com
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From javis at burnham-inst.org Sat Jan 22 08:06:19 2000
From: javis at burnham-inst.org (Jackie Avis)
Date: Wed Aug 20 11:55:24 2003
Subject: Microinjectionist Available
Message-ID: <l03130301b4af0e7654b2@[172.16.13.147]>

Dear members,

I am seeking a position in a transgenic/reproductive biology laboratory
in the San Diego area where I can utilize my embryo manipulation skills. I
have 15 years of experience manipulaing mammalian embryos (mouse, rabbit,
cow, sheep, pig, goat, and monkey) and most recently spent over 10 years
managing the transgenic core facility that I established at a nonprofit
cancer center.
In addition to generating transgenic and knock out mice, I have many
years of experience in embryo manipulation methods including embryo
splitting, embryo aggregation, embryo cryopreservation, IVF, and most
recently sperm freezing (mouse). I have a M.S. degree in reproductive
physiology from U.C. Davis, excellent communication skills, and outstanding
references. Supervisory position is preferred.

Jacqueline Avis, M.S.
Manager, Transgenic Mouse Laboratory
Mouse Molecular Genetics

The Burnham Institute
10901 North Torrey Pines Road
La Jolla, CA 92037

email:javis@burnham-inst.org
phone: (858) 646-3100 X3262

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Martin.HOLZENBERGER at st-antoine.inserm.fr Mon Jan 24 19:02:23 2000
From: Martin.HOLZENBERGER at st-antoine.inserm.fr (Martin Holzenberger)
Date: Wed Aug 20 11:55:24 2003
Subject: impact TRANSGENICS
Message-ID: <l03020903b4b23ece77ed@[195.83.45.81]>

Dear List,

Does anybody know the impact factor/journal citation index of TRANSGENICS.

Thanks,

Martin

______________________________________________________________________

Dr. Martin Holzenberger
INSERM Unit? 515 ?CROISSANCE, DIFFERENCIATION ET PROCESSUS TUMORAUX?
Directeur : Pr Yves LE BOUC
H?pital Saint-Antoine
184, rue du Faubourg Saint-Antoine
F-75571 Paris Cedex 12 (FRANCE)

+ 33 1 49 28 46 29 (Office)
+ 33 1 49 28 46 68 (Lab)
+ 33 1 43 43 10 65 (Fax)
______________________________________________________________________

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From greign at EM.AGR.CA Mon Jan 24 14:08:36 2000
From: greign at EM.AGR.CA (Neva Greig)
Date: Wed Aug 20 11:55:24 2003
Subject: transgenic products on the market
Message-ID: <s88c5e2e.076@EM.AGR.CA>

My son, Jackson is looking for transgenic animal information for a grade six speech.

In particular he would like a list of transgenic products on the market, a better understanding of the environmental concerns and a perspective on what is possible in the future. Can you direct us to quick access of this information? He does not have a lot of time to order books and publications.

I have been able to help somewhat as my work involves plant resistance through transgenic breeding. However, current and simplified information has been a stumbling block.

Thank you for you time and assistance.

Sincerely,
Neva

Neva Greig
Agriculture and Agri-food Canada
Vineland, Ontario,
Canada, L0S 1C0
greign@em.agr.ca
905-562-4113

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Mon Jan 24 19:29:06 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Wed Aug 20 11:55:24 2003
Subject: transgenic products on the market
References: <s88c5e2e.076@EM.AGR.CA>
Message-ID: <388CEE51.65F3E256@karmanos.org>

Neva Greig wrote:

> My son, Jackson is looking for transgenic animal information for a grade six speech. In particular he would like a list of transgenic products on the market, a better understanding of the environmental concerns and a perspective on what is possible in the future. Can you direct us to quick access of
> this information? He does not have a lot of time to order books and publications.
>

Neva,
I hope he has some time to read, either at the library or on the WWW.

Your son may find some good material in the article which appeared in Science last Friday by Austrian Alexander Haslberger:

http://www.sciencemag.org/cgi/content/full/287/5452/431 or

Volume 287, Number 5452 Issue of 21 Jan 2000, pp. 431 - 432
?2000 by The American Association for the Advancement of Science.

It is rich in hyperlinks, and focuses both on the American situation with genetically-modified organisms (GMOs) in foodstuffs and on the situation in western Europe, where bans have been implemented and public awareness is at a level much higher than seen in the U.S.

* * * * * * * * * * * * * * * * * *
Anton-Scott Goustin, Ph.D. T H I N K S N O W
Assistant Professor
Program in Molecular Biology and Genetics
Karmanos Cancer Institute
110 East Warren (Prentis 311)
Detroit, Michigan USA 48201-1379
PHONE 313-833-0715 x2384
FAX 313-832-7294
* * * * * * * * * * * * * * * * * *

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From T.Holtmaat at nih.knaw.nl Tue Jan 25 09:11:54 2000
From: T.Holtmaat at nih.knaw.nl (T.Holtmaat@nih.knaw.nl)
Date: Wed Aug 20 11:55:24 2003
Subject: thy-1 promoter
Message-ID: <200001250906.JAA20112@ns1.ioi.knaw.nl>

Dear Cristina,

The Thy1 promoter has successfully used by us
and others to
generate transgenic mice expressing the transgene in
a
neuronspecific way with post natal onset.
The construct has intron three deleted in order to
confine
expression to the nervous system.
Good references are:
Gordon et al., Cell 50 p445 (1987)
Vidal et al., EMBO J 9 p833 (1990)
Aigner et al., Cell 83 p269 (1995)
Caroni, J. Neurosci. Meth. 71 p3 (1997)

Regards

Anthony Holtmaat
Neth. Inst. Brain Res.
Amsterdam
The Netherlands

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Lutz.Bunger at ed.ac.uk Tue Jan 25 11:47:37 2000
From: Lutz.Bunger at ed.ac.uk (Lutz Bunger)
Date: Wed Aug 20 11:55:24 2003
Subject: Postdoc position at ICAPB University of Edinburgh
Message-ID: <200001251147.LAA19240@holyrood.ed.ac.uk>

University of Edinburgh

Division of Biological Sciences

Institute of Cell, Animal and Population Biology

Post Doctoral Research Fellow in Animal Genetics

Applications are invited for a postdoctoral RF to work on the
genetics of growth and fatness (see { HYPERLINK
http://www.ed.ac.uk/~eang17/mice.html)
}http://www.ed.ac.uk/~eang17/mice.html) with Prof. W.G. Hill, Dr.
L B?nger and Dr. P. D. Keightley (ICAPB) and staff of the Roslin
Institute. This project is funded by BBSRC and provides an
opportunity to carry out genetical analysis of QTLs and candidate
genes in a unique set of selected and inbred lines.
The successful applicant will be responsible for development of and
research using the mouse lines. Candidates should have relevant
experience in e.g. quantitative genetics, molecular biology and
mouse work (preferably with a Home Office Licence). Starting
Salary scale ?16286 - ?21597 pa. The post is available from 1st
April 2000 for 24 months.
Informal enquiries to Dr L.Bunger, tel.0131-650 5442; email: {
HYPERLINK "Lutz.Bunger@ed.ac.uk" }Lutz.Bunger@ed.ac.uk

Please quote ref: 776993
Further particulars including details of the application procedure
should be obtained from the Personnel Department, 1 Roxburgh
Street, Edinburgh EH8 9TB or Tel: 0131-650 2511 (24hr
answering service).
{ HYPERLINK http://www.personnel.ed.ac.uk/recruit.htm
}http://www.personnel.ed.ac.uk/recruit.htm
Closing date: 11 Feb 2000

University of Edinburgh

Division of Biological Sciences

Institute of Cell, Animal and Population Biology

Post Doctoral Research Fellow in Animal Genetics

Further Particulars
Starting Salary scale: ?16286 ? ?21597 pa
Starting date: 1/4/2000 or as soon as possible thereafter
Duration: 24 months
Hours: Full-time (35hrs/week)
Responsible to: Professor W.G.Hill, Dr L.Bunger

Job description:
Background. A Postdoctoral Researcher is required to join a group
working on the genetics of growth, food intake and fatness in mice
(for a summary see { HYPERLINK
http://www.ed.ac.uk/~eang17/mice.html)
}http://www.ed.ac.uk/~eang17/mice.html), and will work closely
with Prof. W.G. Hill and Dr. Lutz B?nger (both ICAPB) and
collaborate with the groups of Dr. P. Keightley (ICAPB) and Prof.
Graham Bulfield of the Roslin Institute. This project provides a
unique opportunity to carry out genetical analysis by QTL mapping
and candidate gene approaches and to elucidate the
physiological background of changes resulting from long-term
divergent selection. Backcross experiments are underway or will
be started in which genes with major effects (known mutations
such as db and ob genes, QTL found in mapping approaches or
transgenes knocking out certain metabolic pathways will be
repeatedly backcrossed into the genetic background of the
different selection lines, to identify the importance of certain
metabolic pathways for observed line divergence. The research
has been funded continously since 1980.

Responsibilities.
Maintenanceand further development of the long-term selected
mouse lines and the derived inbred lines

Supervision and involvement of/in running experiments, data
collection, analysis and interpretation of the data

Design, planning and preparation of new experiments

Maintenance and development of collaborative work using the
unique genetic resources

Involvementin work on other QTL mapping projects in mice
involving major genes

Presentation of work at conferences, writing up for publications.

Managementof technical staff

To undertake any other reasonable and appropriate duties as may
be required

Candidates
We are seeking a candidate with experience of experimental work
in some area ranging from physiological and molecular genetical
studies to population genetical experiments. An interest in working
with mice is essential. Knowledge of quantitative genetics, of
molecular biology techniques and PC literacy (e.g. MS Excel and
MS Word) will be an advantage. A valid Home Office Certificate to
run experiments would be an advantage.

Application procedure
Please complete and return the application form, 3 copies of your
CV and the Equal Opportunities Monitoring Form to Recruitment,
Personnel Department, 1 Roxburgh Street, Edinburgh EH8 9TB by
the advertised closing date.

The University of Edinburgh
The Division of Biological Sciences. The Division comprises three
institutes: Institute of Cell and Molecular Biology (ICMB), Institute
of Cell, Animal, and Population Biology (ICAPB), Institute of
Ecology and Resource Management (IERM), along with the Centre
for Genome Research (CGR). The Division comprises the largest
biological sciences group in the UK. All Institutes in the Division
were awarded a Grade 5 in the 1996 Research Assessment
Exercise, and Biology was rated excellent in the recent Teaching
Quality Assessment Exercise.

Institute of Cell Animal, and Population Biology. The appointee will
be based at ICAPB, located in the Kings Buildings Campus,
alongside its sister Institutes and other departments of the Faculty
of Science and Engineering. Its staff comprises 5 Professors, 13
Readers/Senior Lecturers, 4 lecturers, and 57 Postdoctoral or
Honorary Fellows and Research Assistants. The appointee will
have the opportunity to interact with scientists within the Institute,
other Institutes within the Division, and elsewhere in the Edinburgh
area where there are large groups working on mouse genetics
(e.g. the Roslin Institute).

Lutz Bunger
ICAPB, Kings Buildings
The University of Edinburgh
Edinburgh EH9 3JT
Scotland

Tel 44 (0)131 650 5442
Fax 44 (0)131 667 3210
Email Lutz.Bunger@ed.ac.uk
http://www.ed.ac.uk/~eang17/mice.html
http://helios.bto.ed.ac.uk/icapb/staff/frames.html

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From J.Bowles at cmcb.uq.edu.au Wed Jan 26 16:56:48 2000
From: J.Bowles at cmcb.uq.edu.au (Jo Bowles)
Date: Wed Aug 20 11:55:24 2003
Subject: Cre mice in AUSTRALIA
Message-ID: <v03102808b4b44a85edee@[130.102.68.108]>

Re - recent discussion of Cre mice that can be used for germ line removel
of LoxP-flanked sequences -

1. EIIa-Cre mice (source: Heiner Westphal's group at
NIH), these mice are perfect for partial recombination
events, i.e. removal of neo cassette after 3-loxP
targeting strategy.
2. Zp3-Cre mice (I guess G. Martin's group in CA)
3. MMTV-Cre mice (Line A), 100% female germline
recombination, always complete recombination - no
partial recombination observed, I gave those mice to
the Jax Lab. They distribute this MMTV-Cre line using
this code:
129Bl6(TgN-MMTV-Cre)Mam1

Does anyone in Australia have any of these lines, or any which could also
be used for this purpose???
Thanks all, Jo

Josephine Bowles
Centre for Molecular and Cellular Biology
The University of Queensland
St. Lucia, 4072, Qld,
AUSTRALIA
Ph: +61 7 3365 4566 (GMT+9hrs)
FAX: +61 7 3365 4388

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From STEWARV at ccf.org Wed Jan 26 11:25:53 2000
From: STEWARV at ccf.org (Valerie Stewart, M.S.)
Date: Wed Aug 20 11:55:24 2003
Subject: microchip ids
Message-ID: <s88ed9e0.045@cesmtp.ccf.org>

Hello all--

I have an investigator who wants to invest in a microchip id system for his transgenic animals. He is trying to decide between the AVID animal id system made by Mini-Mitter of Sunriver, Oregon, and the ELAM system made by BioMedic Data Systems of Seaford, Delaware. Does anyone have any experience with either of these systems? Any preferences? Thanks in advance,

Valerie Stewart
Director, Experimental Animal Services
Lerner Research Institute
Cleveland, OH
"stewarv@ccf.org"

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From mlp at informatics.jax.org Wed Jan 26 14:17:40 2000
From: mlp at informatics.jax.org (Moyha Lennon-Pierce)
Date: Wed Aug 20 11:55:24 2003
Subject: Impact factor etc of Transgenics
Message-ID: <v04003a0db4b4ef66e73b@[192.233.41.18]>

Sorry, I deleted the original email requesting impact and citation factor
of the journal Transgenics. Anyway here's the info you requested which was
supplied by our Librarian:

Journal: Transgenics
Impact Factor: 0.789
Cites in 1998 to articles published in 1996 - 12, 1997 - 3

# of articles published in 1996 - 10, 1997 - 9

Cites to recent articles = 15
Number of recent articles = 19 = 0.789

Moyha
Mouse Genome Informatics
The Jackson Laboratory
mailto:mlp@informatics.jax.org

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Wed Jan 26 15:54:02 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Wed Aug 20 11:55:24 2003
Subject: Impact factors
References: <v04003a0db4b4ef66e73b@[192.233.41.18]>
Message-ID: <388F5EEA.C0F49F72@karmanos.org>

Moyha Lennon-Pierce wrote:

> Journal: Transgenics
> Impact Factor: 0.789

That's low! The top-rated journal according to ISI is Nature Genetics, with an
impact factor of ~38 (beats Cell and Nature now). This means that a paper in
Nature Genetics has ~50X the impact of a paper in Transgenics.

Oh, to have a lucky "cool" phenotype! (after all the work and $ put in to
making the mouse). Luck is the word, truly.

Does anybody know how these numbers compare to Mammalian Genome, another place
one sees Tg and KO mice?

* * * * * * * * * * * * * * * * * *
Anton-Scott Goustin, Ph.D.
Assistant Professor
Program in Molecular Biology and Genetics
Karmanos Cancer Institute
110 East Warren (Prentis 311)
Detroit, Michigan USA 48201-1379
PHONE 313-833-0715 x2384
FAX 313-832-7294
* * * * * * * * * * * * * * * * * *

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From FOX_NILES at LILLY.COM Wed Jan 26 15:52:02 2000
From: FOX_NILES at LILLY.COM (Niles Fox)
Date: Wed Aug 20 11:55:24 2003
Subject: Job opening: Eli Lilly & Company
Message-ID: <05256872.0072A235.00@aammta1.d51.lilly.com>

Job opening: Animal technician.

The Research Technologies & Product Development Division of Lilly
Research Laboratories is seeking an individual with small animal handling
and experimentation skills. Minimum educational requirements for this
position are a Veterinary Technology Degree or equivalent. This person
will join a multidisciplinary team involved in new gene discovery and
animal model development. Responsibilities will include conducting
experiments on small animals including transgenic mice and will involve
procedures such as animal dosing, blood and tissue collection, surgery,
in vitro assays and data collection. Candidates should have good
communication and organizational skills and some prior laboratory
experience and computer skills. Prior experience with transgenic mouse
husbandry and record keeping would be helpful. The opening is a four
year term position with the possibility of renewal.

Individuals interested in applying may contact or submit their resume to:

Dr. Niles Fox
Lilly Research Labs
Lilly Corporate Center
Indianapolis, IN 46285
317-276-5415
Email: n.fox@lilly.com

Eli Lilly & Company is an equal opportunity employer committed to
diversity and the strength it brings to the workplace.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Wed Jan 26 15:54:29 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Wed Aug 20 11:55:24 2003
Subject: Impact factors
References: <v04003a0db4b4ef66e73b@[192.233.41.18]>
Message-ID: <388F5F05.9F5E1310@karmanos.org>

Moyha Lennon-Pierce wrote:

> Journal: Transgenics
> Impact Factor: 0.789

Oh, to have a lucky "cool" phenotype! (after all the work and $ put in to
making the mouse). Luck is the word, truly.

Does anybody know how these numbers compare to Mammalian Genome, another place
one sees Tg and KO mice?

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From minerj at pcg.wustl.edu Wed Jan 26 15:38:05 2000
From: minerj at pcg.wustl.edu (Jeff Miner)
Date: Wed Aug 20 11:55:24 2003
Subject: MacMice Program
Message-ID: <l03130306b4b5197fd51a@[128.252.140.231]>

I am writing to find out whether anyone has had problems with the MacMice
4/PPC program or can help us solve ours. We have been using this program
for two and half years and like it very much. However, 6 months ago we had
to start a new database because our old one began behaving strangely and
stopped accepting new entries, presumably because it somehow became
corrupted (accumulated mutations?).

Now, we have run into problems again. Something has happened to the
database that affects the application itself, so that when we try to open a
back up older database the program crashes. Also, when we Create a New
Litter there are always four mice listed as potential parents even before
we enter a cage number, but things return to normal once a cage number is
entered. These two problems appeared at the same time. There have been
other minor incidents, such as an inability to inactivate the first active
litter from one computer but not from another. (We share our database over
a network.)

Thanks,

Jeffrey H. Miner, Ph.D
Assistant Professor of Medicine and
of Cell Biology and Physiology
Renal Division, Box 8126
Washington University School of Medicine
660 South Euclid Ave.
St. Louis, MO 63110
minerj@pcg.wustl.edu

Phone: 314-362-8235
FAX: 362-8237

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From J.Bowles at cmcb.uq.edu.au Thu Jan 27 08:14:50 2000
From: J.Bowles at cmcb.uq.edu.au (Jo Bowles)
Date: Wed Aug 20 11:55:24 2003
Subject: Impact factor Mammalian Genome
In-Reply-To: <388F5EEA.C0F49F72@karmanos.org>
References: <v04003a0db4b4ef66e73b@[192.233.41.18]>
Message-ID: <v03102800b4b522116e24@[130.102.68.108]>

Mammalian Genome impact factor 1.946

>Moyha Lennon-Pierce wrote:
>
>> Journal: Transgenics
>> Impact Factor: 0.789
>
>That's low! The top-rated journal according to ISI is Nature Genetics,
>with an
>impact factor of ~38 (beats Cell and Nature now). This means that a paper in
>Nature Genetics has ~50X the impact of a paper in Transgenics.
>
>Oh, to have a lucky "cool" phenotype! (after all the work and $ put in to
>making the mouse). Luck is the word, truly.
>
>Does anybody know how these numbers compare to Mammalian Genome, another place
>one sees Tg and KO mice?
>
>* * * * * * * * * * * * * * * * * *
>Anton-Scott Goustin, Ph.D.
>Assistant Professor
>Program in Molecular Biology and Genetics
>Karmanos Cancer Institute
>110 East Warren (Prentis 311)
>Detroit, Michigan USA 48201-1379
>PHONE 313-833-0715 x2384
>FAX 313-832-7294
>* * * * * * * * * * * * * * * * * *
>
>
>Content-Type: text/x-vcard; charset=us-ascii;
> name="goustina.vcf"
>Content-Transfer-Encoding: 7bit
>Content-Description: Card for Anton Scott Goustin
>Content-Disposition: attachment;
> filename="goustina.vcf"
>
>Attachment converted: Jo Files:goustina.vcf 6 (TEXT/ttxt) (000001BA)

Josephine Bowles
Centre for Molecular and Cellular Biology
The University of Queensland
St. Lucia, 4072, Qld,
AUSTRALIA
Ph: +61 7 3365 4566 (GMT+9hrs)
FAX: +61 7 3365 4388

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From bll8 at cornell.edu Thu Jan 27 13:08:23 2000
From: bll8 at cornell.edu (Bonnie L. Lamkin)
Date: Wed Aug 20 11:55:24 2003
Subject: Position Available
Message-ID: <3.0.3.32.20000127130823.006c8304@postoffice.mail.cornell.edu>

Tenure-Track Position, Experimental Mouse Biology.

The Department of Biomedical Sciences in the College of Veterinary Medicine
at Cornell University invites applications for a tenure track faculty
position at the level of Assistant or Associate Professor. The successful
candidate will be expected to develop an extramurally funded biomedical
research program and contribute to departmental initiatives in mouse
pathology. A doctoral degree in a biological field (PhD, DVM, MD, or
equivalent) is required. Postgraduate training and/or experience in the
generation, phenotyping, or experimental use of transgenically manipulated
mouse models for scientific research is highly desirable.

Applicants should send a letter of interest, curriculum vitae, and the
names of three referees to Dr. James MacLeod, c/o Ms. Amy Pellegrino,
Department of Biomedical Sciences, College of Veterinary Medicine, Cornell
University, Ithaca, NY 14853-6401. Questions or requests for an expanded
position description can be submitted by email at ap14@cornell.edu or by
telephone at 607-253-3336. The screening of candidates will begin March 1,
2000 and will continue until a suitable candidate is identified. Rank and
salary will be commensurate with the successful candidate's academic
credentials and experience. Cornell University is an Affirmative Action,
Equal Opportunity Employer.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From michael.samuel at ludwig.edu.au Fri Jan 28 09:48:19 2000
From: michael.samuel at ludwig.edu.au (Michael Samuel)
Date: Wed Aug 20 11:55:24 2003
Subject: Immunohistology antibodies
Message-ID: <805AD25D3702D311805900062B004E4263971C@keeper.ludwig.edu.au>

Hi everyone,

I have searched through the archives of this list to find out if this
question has been asked before. I haven't found anything so far, so here
goes. My apologies if it's something that's come up ad nauseum.

I am planning a project to overexpress a transgene in mouse colonic
epithelium and would like to include a tag with the transgene so that I can
detect the protein product easily in Westerns and immunohistological
sections. I am presented with quite a choice here. FLAG, c-myc, hexa-HIS,
HA... Does anyone have information on the relative effectiveness of these
tags in tissue sections? I could use either fixed or frozen sections, but
would prefer fixed.

Thanks in advance for your help.

Michael

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jpiedrahita at cvm.tamu.edu Fri Jan 28 09:23:49 2000
From: jpiedrahita at cvm.tamu.edu (Jorge Piedrahita)
Date: Wed Aug 20 11:55:24 2003
Subject: Position Open
Message-ID: <s891603d.066@cvm.tamu.edu>

Microinjection position available to assist in providing gene targeting and pronuclear injection services. Experience in micromanipulation a must. The position is not on a core facility but a research laboratory. Thus the individual hired will have opportunities for working in other research projects in addition to being responsible for the microinjection. This would be an ideal opportunity for someone interested in eventually going up to graduate school or someone interested in increasing their micromanipulation skills. Our laboratory works both in mice and domestic animals and is adept at generation of chimeras by blastocyst injection, pronuclear injection, and more recently nuclear transfer in the bovine. If interested please contact me at jpiedrahita@cvm.tamu.edu.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jpiedrahita at cvm.tamu.edu Fri Jan 28 10:47:12 2000
From: jpiedrahita at cvm.tamu.edu (Jorge Piedrahita)
Date: Wed Aug 20 11:55:24 2003
Subject: Position Open in Texas
Message-ID: <s89173c7.035@cvm.tamu.edu>

Dr. Jorge A. Piedrahita, PhD
Associate Professor
Department of Veterinary Anatomy and Center for Animal Biotechnology
Texas A&M University
College Station, Texas 77843-4458

409-845-0732 (office)
409-845-9972 (fax)
jpiedrahita@cvm.tamu.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From nstwerra at yahoo.com Sun Jan 30 05:17:17 2000
From: nstwerra at yahoo.com (Kay-Uwe und Ulrike Wagner)
Date: Wed Aug 20 11:55:25 2003
Subject: floxed hygromycin cassette
Message-ID: <20000130131717.19810.qmail@web118.yahoomail.com>

Dear all:

Can somebody provide me with a plasmid containing a
floxed hygromycin cassette (with or without TK
cassette). I would like to use this plasmid to
construct a KO vector.
Thanks. Have a good day.
Kay

Kay-Uwe Wagner
NIH, NIDDK, LGP
Bdg. 8 Rm 107
Bethesda, MD 20892

KUW@nih.gov
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From mcdonald at mayo.edu Mon Jan 31 11:23:19 2000
From: mcdonald at mayo.edu (McDonald, John A., M.D.,Ph.D.)
Date: Wed Aug 20 11:55:25 2003
Subject: Tet responsive GFP Transgenics
Message-ID: <A7B56CCD1E1DD211853D00A0C9A35345028766FF@excsrv05.mayo.edu>

I'm seeking information about a tet-responsive GFP mouse line that might be
available for mapping promoter expression.

Regards,

John A. McDonald, M.D., Ph.D.
SC Johnson Medical Research
Mayo Clinic Scottsdale

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From hofeldtk at hhmi.org Mon Jan 3 19:22:56 2000
From: hofeldtk at hhmi.org (Hofeldt, Kathryn)
Date: Fri Jul 9 14:05:12 2004
Subject: FW: Job Opportunity - Re-advertisement
Message-ID: <7EC621FFC2DFD2119FFF00805FA7C54F3E4CC6@exchange1.hhmi.org>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From pkiela at peds.arizona.edu Mon Jan 3 19:59:35 2000
From: pkiela at peds.arizona.edu (Pawel R. Kiela)
Date: Fri Jul 9 14:05:12 2004
Subject: Workshops
Message-ID: <002b01bf5625$0f4f57e0$97a3c480@peds.arizona.edu>

Hi all,
Does anybody know about workshops on transgenics that are regularly scheduled in the USA ?
I'd appreciate your help.

Pawel

Pawel R. Kiela, D.V.M., Ph.D.
University of Arizona
Department of Pediatrics
1501 N. Campbell Ave.
Tucson, AZ 85724
Ph. (520) 626 7050
Fax: (520) 626 4141

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From WawrousE at intra.nei.nih.gov Mon Jan 3 20:29:34 2000
From: WawrousE at intra.nei.nih.gov (Wawrousek, Eric (NEI))
Date: Fri Jul 9 14:05:12 2004
Subject: Alternatives to embryo transplant surgery?????
Message-ID: <1B3DC68C3FACD31193B200A0C94A4CE20E0557@NEI1701E>

Hello and Happy New Year to all,

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tjf at uci.edu Tue Jan 4 23:52:13 2000
From: tjf at uci.edu (Tom Fielder)
Date: Fri Jul 9 14:05:12 2004
Subject: failed rederivation of mice
Message-ID: <v04003a08b497c2e89ce2@[128.200.21.145]>

Hopefully something obvious will occur to someone out there in tg-land, but
it has me stumped.

Tom

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From ssp at its.caltech.edu Wed Jan 5 00:12:49 2000
From: ssp at its.caltech.edu (Shirley Pease)
Date: Fri Jul 9 14:05:12 2004
Subject: failed rederivation of mice
In-Reply-To: <v04003a08b497c2e89ce2@[128.200.21.145]>
Message-ID: <Pine.GSO.4.05.10001041612110.3082-100000@sue>

Hi Tom,

I would have implanted the 2 cell embryos into day 0.5 recips. I wonder
if that was your problem?

Best, Shirley.

Shirley Pease,
Director, Transgenic and Knockout Core Facility,
California Institute of Technology,
Division of Biology, 147-75
Pasadena,
California, 91125
Tel 626 395 3996
Fax 626 449 0756

On Tue, 4 Jan 2000, Tom Fielder wrote:

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From silvacub at its.caltech.edu Wed Jan 5 00:33:29 2000
From: silvacub at its.caltech.edu (Juan C. Silva)
Date: Fri Jul 9 14:05:12 2004
Subject: failed rederivation of mice
In-Reply-To: <v04003a08b497c2e89ce2@[128.200.21.145]>
Message-ID: <Pine.GSO.4.05.10001041631580.7600-100000@inky>

Hi Tom,
The only thing I would do different is to implant the embryos into 0.5
females.
Juan

<:3_)~~~ <:3_)~~~ <:3_)~~~ <:3_)~~~ <:3_)~~~ <:3_)~~~ <:3_)~~~

On Tue, 4 Jan 2000, Tom Fielder wrote:

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From josepha at amgen.com Wed Jan 5 16:19:24 2000
From: josepha at amgen.com (Anderson, Joe)
Date: Fri Jul 9 14:05:12 2004
Subject: Failed rederivations
Message-ID: <401D3C121A10D3118A7F0008C7CF080E2C6AC6@tan-exch.amgen.com>

Good morning Tom,

Hope this is of some help.

Joe Anderson
Josepha@amgen.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jdl at po.cwru.edu Wed Jan 5 16:50:16 2000
From: jdl at po.cwru.edu (Jackie Nicholson)
Date: Fri Jul 9 14:05:12 2004
Subject: failed rederivations
Message-ID: <l03130307b49926338cb3@[129.22.89.103]>

Wasn't there just a discussion about MHV needing an O/N incubation for
rederivation?

I agree with all others, 0.5d recipients should have been used.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tsaunder at umich.edu Wed Jan 5 18:10:12 2000
From: tsaunder at umich.edu (Thom Saunders)
Date: Fri Jul 9 14:05:12 2004
Subject: failed rederivation of mice
In-Reply-To: <v04003a08b497c2e89ce2@[128.200.21.145]>
Message-ID: <66532.3156066612@host-83.subnet-31.med.umich.edu>

Hi Tom,

I agree with the others, if you had used day 0.5 recipients you would have
had pregnancies.

Sincerely,

Thom Saunders, Ph.D.
Transgenic Animal Model Core
University of Michigan Medical School
Ann Arbor, MI 48109-0674

email: tsaunder@umich.edu
URL: http://www.med.umich.edu/tamc/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jdl at po.cwru.edu Wed Jan 5 19:30:13 2000
From: jdl at po.cwru.edu (Jackie Nicholson)
Date: Fri Jul 9 14:05:12 2004
Subject: mycoplasma not MHV
Message-ID: <l0313030fb4994be0681e@[129.22.89.103]>

Sorry list! I checked my archives, it was mycoplasma that requires an
overnight culture, not MHV.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jklohse at facstaff.wisc.edu Thu Jan 6 00:06:30 2000
From: jklohse at facstaff.wisc.edu (Jan K. Lohse)
Date: Fri Jul 9 14:05:12 2004
Subject: failed rederivation of mice
In-Reply-To: <v04003a08b497c2e89ce2@[128.200.21.145]>
Message-ID: <v04011702b4998c1ea0ff@[128.104.215.81]>

Dr. Jan K. Lohse
Senior Research Specialist
Department of Pathobiological Sciences
School of Veterinary Medicine
2015 Linden Drive West
Madison, WI 53706
(608) 263-9748

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Kristina.Vintersten at EMBL-Heidelberg.de Thu Jan 6 06:26:53 2000
From: Kristina.Vintersten at EMBL-Heidelberg.de (Kristina Vintersten)
Date: Fri Jul 9 14:05:12 2004
Subject: failed rederivation of mice
In-Reply-To: <66532.3156066612@host-83.subnet-31.med.umich.edu>
References: <v04003a08b497c2e89ce2@[128.200.21.145]>
Message-ID: <l03130300b499e4cd9adf@[194.94.44.118]>

Dear Tom,

Best regards

Kristina

______________________________________________________________________

EMBL Transgenic Service
Kristina Vintersten Phone : +49 / 6221 / 387 514
Meyerhofstr. 1 Fax : +49 / 6221 / 387 306
D-69012 Heidelberg E-mail: vinterst@embl-heidelberg.de
GERMANY

http://www.embl-heidelberg.de/ExternalInfo/transgenicService/

_ _
(_)-(_)
_________________________ \"/ ________________________________________
=V=

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From tjf at uci.edu Thu Jan 6 17:28:04 2000
From: tjf at uci.edu (Tom Fielder)
Date: Fri Jul 9 14:05:12 2004
Subject: summary of rederivation responses
Message-ID: <v04003a02b49a0bf30fb3@[128.200.21.145]>

Tom

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tjf at uci.edu Thu Jan 6 19:37:12 2000
From: tjf at uci.edu (Tom Fielder)
Date: Fri Jul 9 14:05:12 2004
Subject: cryopreservation fees survey
Message-ID: <v04003a08b49a2ac64dca@[128.200.21.145]>

What do you charge for freezing 8-cell stage mouse embryos?

How many donors and/or studs do you ask the client to supply?

How do you recover the storage costs (e.g., liquid nitrogen, labor, etc.) -
is this billed on a yearly basis, or do you charge it as one lump sum up
front?

What do you charge for reconstituting a frozen line? Is this billed as a
separate service?

Do your fees vary depending on the strain of mouse?

Thanks in advance.

Tom

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From cbrayton at bcm.tmc.edu Fri Jan 7 18:42:04 2000
From: cbrayton at bcm.tmc.edu (Dr. Cory Brayton)
Date: Fri Jul 9 14:05:12 2004
Subject: Conference/Course Announcement
Message-ID: <NDBBLJPMFKHCFAODHJDHGEIJCCAA.cbrayton@bcm.tmc.edu>

It has been posted on a couple of NIH websites including
http://www.nih.gov/science/models/mouse/courses/pathmut2000.html

Details including registration forms are at
http://www.afip.org/CLDavis/CLDavis.meetings.htm

Below is the unformatted Announcement and Schedule -- not very pretty but
you should not have trouble opening/reading it.

Please contact me (Cory Brayton) if you would like additional information or
if you would like to have the registration form faxed to you.

-----------------------------------------------------------

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From anna at aretha.jax.org Mon Jan 10 15:02:19 2000
From: anna at aretha.jax.org (Anna Anagnostopoulos)
Date: Fri Jul 9 14:05:12 2004
Subject: [Fwd: knockout poster]
Message-ID: <3879F47A.BCFD2293@aretha.jax.org>

Hello everyone,

Can anyone help Dr. Nottoli finding this poster? Please e-mail your
responses directly to timothy.nottoli@yale.edu

Thanks!

Anna
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From: Timothy Nottoli <Timothy.Nottoli@yale.edu>
Subject: knockout poster
Date: Fri, 07 Jan 2000 16:03:06 -0500
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Url: http://mailman.ic.ac.uk/mailman/private/transgenic-list/attachments/20000110/f115dadc/attachment-0001.eml
From anshin at hotmail.com Mon Jan 10 13:56:51 2000
From: anshin at hotmail.com (Anshin Lanugo)
Date: Fri Jul 9 14:05:12 2004
Subject: [Fwd: knockout poster]
Message-ID: <20000110135652.3534.qmail@hotmail.com>

Dear Tbase Group,
If anyone knows where to get this poster could they email me the info as
well?

Thanks!
anshin@hotmail.com

Tania Grunewald
University of Toronto
Genetics and Molecular Biology

>>Date: Fri, 07 Jan 2000 16:03:06 -0500

>>Dear Tbase folks:

>>Sincerely,

>>Tim Nottoli
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From p.w.elbers at amc.uva.nl Mon Jan 10 15:48:30 2000
From: p.w.elbers at amc.uva.nl (Paul Elbers)
Date: Fri Jul 9 14:05:13 2004
Subject: Announcement: The 2nd International Amsterdam Mouse Symposium
Message-ID: <01BF5B8A.8736E740.p.w.elbers@amc.uva.nl>

Dear Colleagues,

13-15 April 2000, under auspices of the International Society for Heart
Research

2nd International Amsterdam Mouse Symposium;
cardiovascular physiology of mice

http://www.usouthal.edu/ishr/Amst-Mouse.htm

Furthermore there will be poster sessions and an industrial exhibition.
Fifty selected abstracts will be published in a special issue of Basic
Research in Cardiology.

Please visit our website for more information and registration
http://www.usouthal.edu/ishr/Amst-Mouse.htm

Yours Sincerely,

Paul Elbers, MD
organizing committee ' The 2nd International Amsterdam Mouse Symposium'

Academisch Medisch Centrum, Experimentele Anesthesiologie
M.0-010
Meibergdreef 9, 1105 AZ, Amsterdam
tel 5665242, fax 6979004, e-mail p.w.elbers@amc.uva.nl

*****
Come to our 2nd International Amsterdam Mouse Symposium: cardiovascular
physiology of mice
Check all the details on: http://www.usouthal.edu/ishr/Amst-Mouse.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From Cynthia.Gander at stjude.org Mon Jan 10 16:34:08 2000
From: Cynthia.Gander at stjude.org (Gander, Cynthia)
Date: Fri Jul 9 14:05:13 2004
Subject: job opening
Message-ID: <83B4CF76D818D111982000805F31CA5302A3F4B0@sjmemexc1.stjude.org>

Dear Members,

Thanks,

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From cware at u.washington.edu Mon Jan 10 16:45:57 2000
From: cware at u.washington.edu (C. Ware)
Date: Fri Jul 9 14:05:13 2004
Subject: [Fwd: knockout poster]
In-Reply-To: <3879F47A.BCFD2293@aretha.jax.org>
Message-ID: <Pine.A41.4.10.10001100845080.204720-100000@homer19.u.washington.edu>

The poster comes from Jackson Labs. I forget it's title, but it mentioned
in their most recent newsletter.

On Mon, 10 Jan 2000, Anna Anagnostopoulos wrote:

> Hello everyone,
>
> Can anyone help Dr. Nottoli finding this poster? Please e-mail your
> responses directly to timothy.nottoli@yale.edu
>
> Thanks!
>
> Anna
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From mmm at cnio.es Mon Jan 10 17:22:52 2000
From: mmm at cnio.es (Marcos.Malumbres)
Date: Fri Jul 9 14:05:13 2004
Subject: [Fwd: knockout poster]
Message-ID: <F0DF64776D4DD31181F400A0C9DB2829044A17@oncogen.cnio.es>

The title of the poster is: "Gene Targeting with 129 strains" and it's
distributed by The Jackson Laboratory. You can contact:

JAX Mice Orders and Technical Support
Tel. 800-422-MICE or 207-288-5845
Fax 207-288-6150
http://www.jax.org/jaxmice

________________________________________
Marcos Malumbres, Ph.D.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From janice_penney at hotmail.com Mon Jan 10 17:25:27 2000
From: janice_penney at hotmail.com (Janice Penney)
Date: Fri Jul 9 14:05:13 2004
Subject: mouse milking
Message-ID: <20000110172527.57012.qmail@hotmail.com>

Dear list

My first question is: Does anyone know a relatively easy way for one person
to get over 50 uL of milk from 6 lactating mice in one day, without
anasthetizing them?

Please reply directly to me and I will send a summary to the list if people
are interested. Thanks very much for any suggestions.

Janice

Janice Penney

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From fmargoli at umaryland.edu Mon Jan 10 19:22:46 2000
From: fmargoli at umaryland.edu (Frank Margolis)
Date: Fri Jul 9 14:05:13 2004
Subject: polycystic ovaries
Message-ID: <v04210114b49fdd9d234d@[134.192.145.156]>

>Dear Colleagues

Has this been a general observation in older mice of the 129 lines or
in other KO lines or a hint of dysfunction associated with our KO?

Any input appreciated

Frank

Frank L. Margolis Ph.D.
Professor
Department of Anatomy and Neurobiology
HSF280
University of Maryland School of Medicine
685 West Baltimore Street
Baltimore MD 21201

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From josepha at amgen.com Mon Jan 10 22:05:54 2000
From: josepha at amgen.com (Anderson, Joe)
Date: Fri Jul 9 14:05:13 2004
Subject: mouse milking
Message-ID: <401D3C121A10D3118A7F0008C7CF080E2C6ACC@tan-exch.amgen.com>

Hi Janet,

I have some info for you but your address at hotmail keeps bouncing the
message back to me with an "account inactive" error.

Do you have another address?

Joe Anderson
Josepha@amgen.com <mailto:Josepha@amgen.com>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From ccl at aretha.jax.org Tue Jan 11 19:21:22 2000
From: ccl at aretha.jax.org (Carol Cutler Linder)
Date: Fri Jul 9 14:05:13 2004
Subject: Gene Targeting with 129 strains
Message-ID: <v03007817b4a1311c1278@[209.194.200.19]>

Hi List,

You can email me directly (ccl@jax.org) with a complete mailing address and
I will forward the request on to Customer Service or you can contact
Customer Service by phone.

Best regards,
Carol

Marcos Malumbres, Ph.D. wrote:
The title of the poster is: "Gene Targeting with 129 strains" and it's
distributed by The Jackson Laboratory. You can contact:

JAX Mice Orders and Technical Support
Tel. 800-422-MICE or 207-288-5845
Fax 207-288-6150
http://www.jax.org/jaxmice

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From lin_gan at urmc.rochester.edu Wed Jan 12 17:45:37 2000
From: lin_gan at urmc.rochester.edu (Lin Gan)
Date: Fri Jul 9 14:05:13 2004
Subject: C57BL6 ES cells
Message-ID: <v03110701b4a26d99b61b@[128.151.151.198]>

Dear List,

I am looking for a source for the C57BL6 ES cell lines. I would
appreciate your input and any experience/advice regarding the C57BL ES
cells.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From DJ_Kappes at fccc.edu Thu Jan 13 15:55:02 2000
From: DJ_Kappes at fccc.edu (Dr. Dietmar Kappes)
Date: Fri Jul 9 14:05:13 2004
Subject: Transgenic and knockout positions
Message-ID: <B4A35F86@arir.fccc.edu>

Fox Chase Cancer Center in Philadelphia has 2 job openings for individuals
skilled in transgenic and ES cell work. See descriptions below.

Direct inquiries to: Dr. Dietmar Kappes, Fox Chase Cancer Center, 7701 Burholme
Avenue, Philadelphia 19111; Tel.: 215-728-5374; E-mail: dj_kappes@fccc.edu.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From kellek01 at doc.mssm.edu Thu Jan 13 18:20:11 2000
From: kellek01 at doc.mssm.edu (Kevin Kelley)
Date: Fri Jul 9 14:05:13 2004
Subject: culturing of C57Bl/6 eggs
Message-ID: <387E0D7B.1924@doc.mssm.edu>

Dear list,

Thank you,
Kevin A. Kelley, Ph.D.
Associate Director, Center for Laboratory Animal Sciences
Director, Transgenic Services

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From kmoore at animal.ufl.edu Thu Jan 13 19:33:36 2000
From: kmoore at animal.ufl.edu (Karen Moore)
Date: Fri Jul 9 14:05:13 2004
Subject: Position Available
Message-ID: <000701bf5dfd$160ca620$3304e30a@nagapc.dps.ufl.edu>

Karen Moore, Ph.D.
University of Florida
Department of Dairy and Poultry Sciences
PO Box 110920
Gainesville, FL 32611-0920
(352)392-1958
(352)392-5595 fax
email: kmoore@animal.ufl.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From RobinsoM at pediatrics.ohio-state.edu Thu Jan 13 19:25:00 2000
From: RobinsoM at pediatrics.ohio-state.edu (Robinson, Michael)
Date: Fri Jul 9 14:05:13 2004
Subject: BAC transgenics
Message-ID: <1366FE962DB3D311B8DA00A0C9EA0484099E96@RESMS01.net.chi.ohio-state.edu>

Dear List,

I sincerely appreciate any comments and/or advice about this matter.

Mike Robinson, Ph.D.
Division of Molecular and Human Genetics
Children's Research Institute
700 Children's Drive
Columbus, OH 43205
Phone 614-722-2764
FAX: 614-722-2716

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From nagy at mshri.on.ca Thu Jan 13 21:48:47 2000
From: nagy at mshri.on.ca (Andras Nagy)
Date: Fri Jul 9 14:05:13 2004
Subject: HPRT minus R1
Message-ID: <387E483D.A6ECFB31@mshri.on.ca>

Hi All,

Andras Nagy PhD
Senior Staff Scientist
Mount Sinai Hospital
Samuel Lunenfeld Research Institute
600 University Ave.
Toronto, M5G 1X5
Canada

tel. (416)-586-3246 0ffice
tel. (416)-586-8455 Lab
tel. (416)-586-5361 Secretary
fax (416)-586-8588
URL: http://www.mshri.on.ca/nagy/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Fred.Sablitzky at nottingham.ac.uk Fri Jan 14 10:50:12 2000
From: Fred.Sablitzky at nottingham.ac.uk (Fred Sablitzky)
Date: Fri Jul 9 14:05:13 2004
Subject: position available
Message-ID: <211D16E31A5@pdn1.gene.nottingham.ac.uk>

Post-doctoral position available

fred.sablitzky@nottingham.ac.uk

For more information see:

http://www.ifz.uni-essen.de/eu/Inhaltsverzeichnis.html

http://www.nottingham.ac.uk/genetics/Fred%20Sablitzky.htm

---- NEW address ----

Prof. Fred Sablitzky
The University of Nottingham
Institute of Genetics
Queen's Medical Centre
Nottingham
NG7 2UH

phone: 0115 849 3242
fax: 0115 849 3243
email: fred.sablitzky@nottingham.ac.uk
http://www.nottingham.ac.uk/genetics/Fred%20Sablitzky.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From nagy at mshri.on.ca Fri Jan 14 15:21:21 2000
From: nagy at mshri.on.ca (Andras Nagy)
Date: Fri Jul 9 14:05:13 2004
Subject: Cre recombinase database
Message-ID: <387F3ECE.EBAC775D@mshri.on.ca>

Dear All,

I appreciate any help in keeping this data base absolutely updated. If
you find anything missing, for example, lines which are made, the
information is releasable, please let me know.

Thank,

Andras
nagy@mshri.on.ca

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From DonovanD at grc.nia.nih.gov Fri Jan 14 21:40:05 2000
From: DonovanD at grc.nia.nih.gov (Donovan, Dave)
Date: Fri Jul 9 14:05:13 2004
Subject: dirty facility cleanup
Message-ID: <C5BE6D142223D211909E0000F8CD228A014AC4B0@c3.grc.nia.nih.gov>

Dear List,

Thanks in advance.

Dave

David M. Donovan, Ph.D.
Chief, Transgenic and Knockout Facility Section
Gerontology Research Center, IRP, NIA, NIH
5600 Nathan Shock Drive
Baltimore, MD 21224-6825
410-558-8111 voice
410-558-8236 fax

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From sfk at bilbo.bio.purdue.edu Mon Jan 17 14:28:42 2000
From: sfk at bilbo.bio.purdue.edu (Steve Konieczny)
Date: Fri Jul 9 14:05:13 2004
Subject: Director, Transgenic Facility Position
Message-ID: <4.1.20000117090436.00a0bc60@bilbo.bio.purdue.edu>

Dear Colleagues,

Sincerely,

Stephen F. Konieczny, Professor
Department of Biological Sciences
Purdue University
West Lafayette, IN 47907-1392

Tel: 765-494-7976
Fax: 765-496-2536
email: sfk@bilbo.bio.purdue.edu

____________________________________________________________________________
____________

Research Scientist, Transgenic Mouse Core Facility
Purdue University Cancer Center

Purdue University is an Equal Opportunity/Affirmative Action Employer.

____________________________________________________________________________
____________
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From sauerb at omrf.ouhsc.edu Tue Jan 18 00:25:07 2000
From: sauerb at omrf.ouhsc.edu (Brian Sauer)
Date: Fri Jul 9 14:05:13 2004
Subject: VIVARIUM DIRECTOR Position Opening
Message-ID: <v0311070cb4a90ad49092@[157.142.201.104]>

Dear Colleagues,

************************************************************
VIVARIUM DIRECTOR, DVM

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Johannes.Wilbertz at cmb.ki.se Tue Jan 18 07:43:09 2000
From: Johannes.Wilbertz at cmb.ki.se (Johannes Wilbertz , Genetik)
Date: Fri Jul 9 14:05:13 2004
Subject: Trans-Tech-Meeting Stockholm
Message-ID: <l03110701b4a9c89fc52f@[130.237.120.195]>

Dear Colleagues,

below you can see the preliminary schedule for the Trans-Tech Meeting in
Stockholm.
If someone needs further information, just visit our homepage
(http://www.mousecamp.ki.se).

Regards

Johannes

===================================================

Trans-Tech-Meeting 2000
Stockholm

May 17th, 18th and 19th
2000

Organizers:
Cambridge University / Karolinska Institute

Preliminary Schedule

TALKS

Session: Knockout mice and inducible systems; Genetic II
a) Different lines of ES cells
b) Inducible knockout systems
c) Reporter genes (LacZ, tau/lacZ, GFP etc.)
c) Induced mutagenesis in mice

Session: New techniques in the production of transgenic animals
a) Sperm mediated gene transfer
b) Cloning techniques
c) Tetraploids and aggregation

Session: Production of non-rodent transgenic animals and their use in
biomedical research
a) TG-Chicken Methods and use
b) TG-Fish Strains, methods and use
c) TG-Cattle Strains, methods and use

Session: Transgenic rodents in biomedical research
a) Immunology
b) Hypertension research (Rats)
c) Genetic
d) Pharmacology
e) Cancer research

POSTER PRESENTATIONS

a) Latest findings in transgenic technology
b) Interesting models in biomedical research (rodents and non-rodents)
c) New developments in animal husbandry

=====================================

Johannes Wilbertz, Ph.D.
Head of Campus Transgene Facility
Karolinska Institute
CMB / MouseCamp
Von Eulers v?g 3
171 77 Stockholm
Sweden

Tel. Office: + 46 8 728 7318
Tel. Inj.lab.: + 46 8 728 7613
Tel. mobile: + 46 70 4714985
Fax: + 46 8 308374
E-Mail: johannes.wilbertz@cmb.ki.se

http://www.mousecamp.ki.se/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From chenm0 at uthscsa.edu Tue Jan 18 16:57:33 2000
From: chenm0 at uthscsa.edu (chenm0)
Date: Fri Jul 9 14:05:13 2004
Subject: job wanted
Message-ID: <01JKUIVCPC1GA3ETS3@uthscsa.edu>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Matthias.Ernst at ludwig.edu.au Tue Jan 18 23:55:57 2000
From: Matthias.Ernst at ludwig.edu.au (Matthias Ernst)
Date: Fri Jul 9 14:05:13 2004
Subject: design of transgene construct
Message-ID: <805AD25D3702D311805900062B004E420F4E41@keeper.ludwig.edu.au>

Dear List

Matthias Ernst

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From DonovanD at grc.nia.nih.gov Wed Jan 19 13:49:39 2000
From: DonovanD at grc.nia.nih.gov (Donovan, Dave)
Date: Fri Jul 9 14:05:13 2004
Subject: design of transgene construct
Message-ID: <C5BE6D142223D211909E0000F8CD228A014AC4CC@c3.grc.nia.nih.gov>

Dear Matthias,

-----Original Message-----
From: Matthias Ernst [mailto:Matthias.Ernst@ludwig.edu.au]
Sent: Tuesday, January 18, 2000 6:56 PM
To: 'transgenic-list@ic.ac.uk'
Subject: Re: design of transgene construct

Dear List

Matthias Ernst

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From garofalo at ermes.cba.unige.it Wed Jan 19 15:26:05 2000
From: garofalo at ermes.cba.unige.it (Silvio Garofalo)
Date: Fri Jul 9 14:05:13 2004
Subject: Nikon/Narishige IM-88 injectors
Message-ID: <l03130301b4ab85759d9d@[130.251.106.105]>

Can anybody advice about the Nikon/Narishige IM-88 microinjectors for
pronuclei injection
(http://www.nikon.co.jp/inst/Biomedical/nt88ne/index.html)? Is anybody
using them?

Thanks a lot

Tel.: +39 010 5737 393
Fax: +39 010 5737 405
e-mail: garofalo@ermes.cba.unige.it
===================================================================

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From DJ_Kappes at fccc.edu Wed Jan 19 16:44:52 2000
From: DJ_Kappes at fccc.edu (Dr. Dietmar Kappes)
Date: Fri Jul 9 14:05:13 2004
Subject: No subject
Message-ID: <B4AB5434@arir.fccc.edu>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Paul.Booth at agrsci.dk Wed Jan 19 16:56:55 2000
From: Paul.Booth at agrsci.dk (Paul Booth)
Date: Fri Jul 9 14:05:13 2004
Subject: No subject
Message-ID: <5166A14C6887D111991100805F8BB74903A76A8C@foulum01.agrsci.dk>

From: Paul.Booth@agrsci.dk
Subject: Dishes for morulae aggregation

Email: Paul.Booth@agrsci.dk
Tel: (45) 89 99 12 64
Fax: (45) 89 99 13 00

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From cfillat at supermac.iro.es Wed Jan 19 19:08:26 2000
From: cfillat at supermac.iro.es (fistro)
Date: Fri Jul 9 14:05:13 2004
Subject: Thy-1.2 promoter
Message-ID: <1263817187-26396266@supermac.iro.es>

Dear list,

1)Does any of you has had any experiemce with transgenic mice carrying
this promoter?
2)Does any of you has this prmoter available? or, do you konw who I could
ask for it?

Thank you very much,

Happy 2000!!

Cristina Fillat

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From foleyk at zgi.com Wed Jan 19 17:27:57 2000
From: foleyk at zgi.com (Kevin Foley)
Date: Fri Jul 9 14:05:13 2004
Subject: FW: Thy-1.2 promoter
Message-ID: <200001191823.KAA1029526@stella.zgi.com>

You might also consider the neuron-specific enolase (NSE) promoter, which is
expressed in all differentiated neurons. It has been employed by many
groups in transgenic mice.

Cheers,
Kevin

Kevin P. Foley, Ph.D.
Scientist, Dept. of Genetics
ZymoGenetics, Inc.
1201 Eastlake Avenue East
Seattle, WA 98102
tel: (206) 442-6625
fax: (206) 442-6699
e-mail: foleyk@zgi.com

----------
>From: fistro <cfillat@supermac.iro.es>
>To: <transgenic-list@ic.ac.uk>
>Subject: Thy-1.2 promoter
>Date: Wed, Jan 19, 2000, 12:08 PM
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From damak at inka.mssm.edu Wed Jan 19 18:57:30 2000
From: damak at inka.mssm.edu (Sami Damak)
Date: Fri Jul 9 14:05:13 2004
Subject: Cre mice
Message-ID: <3.0.6.32.20000119135730.007b9690@inka.mssm.edu>

Do you know a source, commercial or not, of Cre mice that can be used for
germ line removal of LoxP-flanked sequences.
Thanks

Sami Damak MD PhD
Research Assistant Professor
Department of Physiology and Biophysics
The Mount Sinai School of Medicine
1 Gustave L. Levy Place
New York NY 10029
Tel: 212-659 8694
Fax: 212-849 2599

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From nstwerra at yahoo.com Wed Jan 19 19:47:20 2000
From: nstwerra at yahoo.com (Kay-Uwe und Ulrike Wagner)
Date: Fri Jul 9 14:05:13 2004
Subject: Cre mice
Message-ID: <20000119194720.23835.qmail@web112.yahoomail.com>

I bet there are many more lines out there that could
be used for this purpose.

Kay

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tsaunder at umich.edu Thu Jan 20 01:07:35 2000
From: tsaunder at umich.edu (Thom Saunders)
Date: Fri Jul 9 14:05:13 2004
Subject: Nikon/Narishige IM-88 injectors
In-Reply-To: <l03130301b4ab85759d9d@[130.251.106.105]>
Message-ID: <182984.3157301255@pm471-00.dialip.mich.net>

Sincerely,

Thom

Thom Saunders, Ph.D.
Transgenic Animal Model Core
University of Michigan Medical School
Ann Arbor, MI 48109

http://www.med.umich.edu/tamc
tsaunder@umich.edu

--On Wed, Jan 19, 2000 4:26 PM +0100 Silvio Garofalo
<garofalo@ermes.cba.unige.it> wrote:

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From murg at fas.harvard.edu Thu Jan 20 01:42:38 2000
From: murg at fas.harvard.edu (Stephanie Murg)
Date: Fri Jul 9 14:05:13 2004
Subject: Thy-1.2 promoter
In-Reply-To: <1263817187-26396266@supermac.iro.es>
Message-ID: <001c01bf62e7$a1dc4cc0$5f9bf78c@leverett.fas.harvard.edu>

*An inducible transgenic system using NSE (using a modified tTa approach):

Transgenic animals with inducible, targeted gene expression in brain.
Chen J, Kelz MB, Zeng G, Sakai N, Steffen C, Shockett PE, Picciotto MR,
Duman RS, Nestler EJ
Mol Pharmacol 1998 Sep 54:3 495-503

Stephanie Murg

____________________
Stephanie Murg
Harvard University
FAX 815.425.1732
TEL 617.493.2802
murg@fas.harvard.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From clandel at jax.org Thu Jan 20 17:16:58 2000
From: clandel at jax.org (Carlisle Landel)
Date: Fri Jul 9 14:05:13 2004
Subject: Thy-1.2 promoter
In-Reply-To: <1263817187-26396266@supermac.iro.es>
References: <1263817187-26396266@supermac.iro.es>
Message-ID: <v04210106b4acf262c444@[199.94.155.139]>

Christina,

You realize, of course, that Thy-1.2 will also go to T cells, right?

I spent some time trying to use Thy-1 as a T-cell directing
transgenic promoter. I'd recommend that you stay away from it. It
apparently has control elements scattered all through the gene.

Regards,

Carlisle Landel

PS: You can check out the following for Thy-1 transgenics:

***********************************************************************
Carlisle P. Landel, Ph.D.
Cryopreservation Laboratory
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
"Cold? It's not cold, just a little chilly."
***********************************************************************
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From JOHN.MURASKI at ROCHE.COM Fri Jan 21 00:47:11 2000
From: JOHN.MURASKI at ROCHE.COM (Muraski, John {Geno~Palo Alto})
Date: Fri Jul 9 14:05:13 2004
Subject: Success of 129
Message-ID: <418F66121D9DD311A54B0000F80197880F6840@rplmsem1.pal.roche.com>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From javis at burnham-inst.org Sat Jan 22 16:06:19 2000
From: javis at burnham-inst.org (Jackie Avis)
Date: Fri Jul 9 14:05:13 2004
Subject: Microinjectionist Available
Message-ID: <l03130301b4af0e7654b2@[172.16.13.147]>

Dear members,

Jacqueline Avis, M.S.
Manager, Transgenic Mouse Laboratory
Mouse Molecular Genetics

The Burnham Institute
10901 North Torrey Pines Road
La Jolla, CA 92037

email:javis@burnham-inst.org
phone: (858) 646-3100 X3262

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Martin.HOLZENBERGER at st-antoine.inserm.fr Mon Jan 24 18:02:23 2000
From: Martin.HOLZENBERGER at st-antoine.inserm.fr (Martin Holzenberger)
Date: Fri Jul 9 14:05:13 2004
Subject: impact TRANSGENICS
Message-ID: <l03020903b4b23ece77ed@[195.83.45.81]>

Dear List,

Does anybody know the impact factor/journal citation index of TRANSGENICS.

Thanks,

Martin

______________________________________________________________________

+ 33 1 49 28 46 29 (Office)
+ 33 1 49 28 46 68 (Lab)
+ 33 1 43 43 10 65 (Fax)
______________________________________________________________________

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From greign at EM.AGR.CA Mon Jan 24 19:08:36 2000
From: greign at EM.AGR.CA (Neva Greig)
Date: Fri Jul 9 14:05:13 2004
Subject: transgenic products on the market
Message-ID: <s88c5e2e.076@EM.AGR.CA>

My son, Jackson is looking for transgenic animal information for a grade six speech.

I have been able to help somewhat as my work involves plant resistance through transgenic breeding. However, current and simplified information has been a stumbling block.

Thank you for you time and assistance.

Sincerely,
Neva

Neva Greig
Agriculture and Agri-food Canada
Vineland, Ontario,
Canada, L0S 1C0
greign@em.agr.ca
905-562-4113

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Tue Jan 25 00:29:06 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Fri Jul 9 14:05:14 2004
Subject: transgenic products on the market
References: <s88c5e2e.076@EM.AGR.CA>
Message-ID: <388CEE51.65F3E256@karmanos.org>

Neva Greig wrote:

Neva,
I hope he has some time to read, either at the library or on the WWW.

Your son may find some good material in the article which appeared in Science last Friday by Austrian Alexander Haslberger:

http://www.sciencemag.org/cgi/content/full/287/5452/431 or

Volume 287, Number 5452 Issue of 21 Jan 2000, pp. 431 - 432
?2000 by The American Association for the Advancement of Science.

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From T.Holtmaat at nih.knaw.nl Tue Jan 25 08:11:54 2000
From: T.Holtmaat at nih.knaw.nl (T.Holtmaat@nih.knaw.nl)
Date: Fri Jul 9 14:05:14 2004
Subject: thy-1 promoter
Message-ID: <200001250906.JAA20112@ns1.ioi.knaw.nl>

Dear Cristina,

Regards

Anthony Holtmaat
Neth. Inst. Brain Res.
Amsterdam
The Netherlands

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Lutz.Bunger at ed.ac.uk Tue Jan 25 11:47:37 2000
From: Lutz.Bunger at ed.ac.uk (Lutz Bunger)
Date: Fri Jul 9 14:05:14 2004
Subject: Postdoc position at ICAPB University of Edinburgh
Message-ID: <200001251147.LAA19240@holyrood.ed.ac.uk>

University of Edinburgh

Division of Biological Sciences

Institute of Cell, Animal and Population Biology

Post Doctoral Research Fellow in Animal Genetics

University of Edinburgh

Division of Biological Sciences

Institute of Cell, Animal and Population Biology

Post Doctoral Research Fellow in Animal Genetics

Responsibilities.
Maintenanceand further development of the long-term selected
mouse lines and the derived inbred lines

Supervision and involvement of/in running experiments, data
collection, analysis and interpretation of the data

Design, planning and preparation of new experiments

Maintenance and development of collaborative work using the
unique genetic resources

Involvementin work on other QTL mapping projects in mice
involving major genes

Presentation of work at conferences, writing up for publications.

Managementof technical staff

To undertake any other reasonable and appropriate duties as may
be required

Lutz Bunger
ICAPB, Kings Buildings
The University of Edinburgh
Edinburgh EH9 3JT
Scotland

Tel 44 (0)131 650 5442
Fax 44 (0)131 667 3210
Email Lutz.Bunger@ed.ac.uk
http://www.ed.ac.uk/~eang17/mice.html
http://helios.bto.ed.ac.uk/icapb/staff/frames.html

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From J.Bowles at cmcb.uq.edu.au Wed Jan 26 06:56:48 2000
From: J.Bowles at cmcb.uq.edu.au (Jo Bowles)
Date: Fri Jul 9 14:05:14 2004
Subject: Cre mice in AUSTRALIA
Message-ID: <v03102808b4b44a85edee@[130.102.68.108]>

Re - recent discussion of Cre mice that can be used for germ line removel
of LoxP-flanked sequences -

Does anyone in Australia have any of these lines, or any which could also
be used for this purpose???
Thanks all, Jo

Josephine Bowles
Centre for Molecular and Cellular Biology
The University of Queensland
St. Lucia, 4072, Qld,
AUSTRALIA
Ph: +61 7 3365 4566 (GMT+9hrs)
FAX: +61 7 3365 4388

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From STEWARV at ccf.org Wed Jan 26 16:25:53 2000
From: STEWARV at ccf.org (Valerie Stewart, M.S.)
Date: Fri Jul 9 14:05:14 2004
Subject: microchip ids
Message-ID: <s88ed9e0.045@cesmtp.ccf.org>

Hello all--

Valerie Stewart
Director, Experimental Animal Services
Lerner Research Institute
Cleveland, OH
"stewarv@ccf.org"

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From mlp at informatics.jax.org Wed Jan 26 19:17:40 2000
From: mlp at informatics.jax.org (Moyha Lennon-Pierce)
Date: Fri Jul 9 14:05:14 2004
Subject: Impact factor etc of Transgenics
Message-ID: <v04003a0db4b4ef66e73b@[192.233.41.18]>

Sorry, I deleted the original email requesting impact and citation factor
of the journal Transgenics. Anyway here's the info you requested which was
supplied by our Librarian:

Journal: Transgenics
Impact Factor: 0.789
Cites in 1998 to articles published in 1996 - 12, 1997 - 3

# of articles published in 1996 - 10, 1997 - 9

Cites to recent articles = 15
Number of recent articles = 19 = 0.789

Moyha
Mouse Genome Informatics
The Jackson Laboratory
mailto:mlp@informatics.jax.org

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Wed Jan 26 20:54:02 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Fri Jul 9 14:05:14 2004
Subject: Impact factors
References: <v04003a0db4b4ef66e73b@[192.233.41.18]>
Message-ID: <388F5EEA.C0F49F72@karmanos.org>

Moyha Lennon-Pierce wrote:

> Journal: Transgenics
> Impact Factor: 0.789

Oh, to have a lucky "cool" phenotype! (after all the work and $ put in to
making the mouse). Luck is the word, truly.

Does anybody know how these numbers compare to Mammalian Genome, another place
one sees Tg and KO mice?

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From FOX_NILES at LILLY.COM Wed Jan 26 20:52:02 2000
From: FOX_NILES at LILLY.COM (Niles Fox)
Date: Fri Jul 9 14:05:14 2004
Subject: Job opening: Eli Lilly & Company
Message-ID: <05256872.0072A235.00@aammta1.d51.lilly.com>

Job opening: Animal technician.

Individuals interested in applying may contact or submit their resume to:

Dr. Niles Fox
Lilly Research Labs
Lilly Corporate Center
Indianapolis, IN 46285
317-276-5415
Email: n.fox@lilly.com

Eli Lilly & Company is an equal opportunity employer committed to
diversity and the strength it brings to the workplace.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Wed Jan 26 20:54:29 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Fri Jul 9 14:05:14 2004
Subject: Impact factors
References: <v04003a0db4b4ef66e73b@[192.233.41.18]>
Message-ID: <388F5F05.9F5E1310@karmanos.org>

Moyha Lennon-Pierce wrote:

> Journal: Transgenics
> Impact Factor: 0.789

Oh, to have a lucky "cool" phenotype! (after all the work and $ put in to
making the mouse). Luck is the word, truly.

Does anybody know how these numbers compare to Mammalian Genome, another place
one sees Tg and KO mice?

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From minerj at pcg.wustl.edu Wed Jan 26 21:38:05 2000
From: minerj at pcg.wustl.edu (Jeff Miner)
Date: Fri Jul 9 14:05:14 2004
Subject: MacMice Program
Message-ID: <l03130306b4b5197fd51a@[128.252.140.231]>

Thanks,

Phone: 314-362-8235
FAX: 362-8237

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From J.Bowles at cmcb.uq.edu.au Wed Jan 26 22:14:50 2000
From: J.Bowles at cmcb.uq.edu.au (Jo Bowles)
Date: Fri Jul 9 14:05:14 2004
Subject: Impact factor Mammalian Genome
In-Reply-To: <388F5EEA.C0F49F72@karmanos.org>
References: <v04003a0db4b4ef66e73b@[192.233.41.18]>
Message-ID: <v03102800b4b522116e24@[130.102.68.108]>

Mammalian Genome impact factor 1.946

Josephine Bowles
Centre for Molecular and Cellular Biology
The University of Queensland
St. Lucia, 4072, Qld,
AUSTRALIA
Ph: +61 7 3365 4566 (GMT+9hrs)
FAX: +61 7 3365 4388

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From bll8 at cornell.edu Thu Jan 27 18:08:23 2000
From: bll8 at cornell.edu (Bonnie L. Lamkin)
Date: Fri Jul 9 14:05:14 2004
Subject: Position Available
Message-ID: <3.0.3.32.20000127130823.006c8304@postoffice.mail.cornell.edu>

Tenure-Track Position, Experimental Mouse Biology.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From michael.samuel at ludwig.edu.au Thu Jan 27 22:48:19 2000
From: michael.samuel at ludwig.edu.au (Michael Samuel)
Date: Fri Jul 9 14:05:14 2004
Subject: Immunohistology antibodies
Message-ID: <805AD25D3702D311805900062B004E4263971C@keeper.ludwig.edu.au>

Hi everyone,

Thanks in advance for your help.

Michael

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jpiedrahita at cvm.tamu.edu Fri Jan 28 15:23:49 2000
From: jpiedrahita at cvm.tamu.edu (Jorge Piedrahita)
Date: Fri Jul 9 14:05:14 2004
Subject: Position Open
Message-ID: <s891603d.066@cvm.tamu.edu>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jpiedrahita at cvm.tamu.edu Fri Jan 28 16:47:12 2000
From: jpiedrahita at cvm.tamu.edu (Jorge Piedrahita)
Date: Fri Jul 9 14:05:14 2004
Subject: Position Open in Texas
Message-ID: <s89173c7.035@cvm.tamu.edu>

Dr. Jorge A. Piedrahita, PhD
Associate Professor
Department of Veterinary Anatomy and Center for Animal Biotechnology
Texas A&M University
College Station, Texas 77843-4458

409-845-0732 (office)
409-845-9972 (fax)
jpiedrahita@cvm.tamu.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From russelld at njc.org Sat Jan 29 15:31:13 2000
From: russelld at njc.org (David Russell)
Date: Fri Jul 9 14:05:14 2004
Subject: New address
Message-ID: <20000129153032015.AAA122.205@[198.243.65.68]>

Here's my new e-mail address.

david.russell@uchsc.edu.

please write

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From nstwerra at yahoo.com Sun Jan 30 13:17:17 2000
From: nstwerra at yahoo.com (Kay-Uwe und Ulrike Wagner)
Date: Fri Jul 9 14:05:14 2004
Subject: floxed hygromycin cassette
Message-ID: <20000130131717.19810.qmail@web118.yahoomail.com>

Dear all:

Can somebody provide me with a plasmid containing a
floxed hygromycin cassette (with or without TK
cassette). I would like to use this plasmid to
construct a KO vector.
Thanks. Have a good day.
Kay

Kay-Uwe Wagner
NIH, NIDDK, LGP
Bdg. 8 Rm 107
Bethesda, MD 20892

KUW@nih.gov
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From mcdonald at mayo.edu Mon Jan 31 18:23:19 2000
From: mcdonald at mayo.edu (McDonald, John A., M.D.,Ph.D.)
Date: Fri Jul 9 14:05:14 2004
Subject: Tet responsive GFP Transgenics
Message-ID: <A7B56CCD1E1DD211853D00A0C9A35345028766FF@excsrv05.mayo.edu>

I'm seeking information about a tet-responsive GFP mouse line that might be
available for mapping promoter expression.

Regards,

John A. McDonald, M.D., Ph.D.
SC Johnson Medical Research
Mayo Clinic Scottsdale

*2000 February

From nagy at mshri.on.ca Wed Feb 2 11:27:20 2000
From: nagy at mshri.on.ca (Andras Nagy)
Date: Wed Aug 20 11:55:25 2003
Subject: your input is needed
Message-ID: <38984CD7.429E0A59@mshri.on.ca>

Dear List,

I am contributing to the new, third edition of the Brigid Hogan's:
"Manipulating the Mouse Embryo; A laboratory manual". The plan is to
make a significant rewriting on the book, which will reflect the changes
occurred in the last six years concerning the transgenic technology of
the mouse.
The list is certainly the best forum to help making the new edition the
best possible fit to the needs of growing community of users and
readers, since everybody on the list knows this book by heart.
I would highly appreciate any comments, helpful and constructive
suggestions concerning the new edition. (For example, what we should
leave out from or add to the new it.)
In the next few weeks I am collecting highly recommendable protocols. If
you have one that you are happy with and want to share this with the
others please email me together with notes which will help us to
evaluate and provide the best possible one for the community.

Thanks very much for your contribution, which will be properly
acknowledged.

Andras Nagy, PhD
Senior Staff Scientist
Mount Sinai Hospital
Samuel Lunenfeld Research Institute
600 University Ave.
Toronto, M5G 1X5
Canada

Email: nagy@mshri.on.ca
tel. (416)-586-3246 0ffice
tel. (416)-586-8455 Lab
tel. (416)-586-5361 Secretary
fax (416)-586-8588
URL: http://www.mshri.on.ca/nagy/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Wed Feb 2 14:05:54 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Wed Aug 20 11:55:25 2003
Subject: 3rd edition of Hogan manual
References: <38984CD7.429E0A59@mshri.on.ca>
Message-ID: <38988012.501677BD@karmanos.org>

Andras Nagy wrote:

> I am contributing to the new, third edition of the Brigid Hogan's:
> "Manipulating the Mouse Embryo; A laboratory manual". The plan is to
> make a significant rewriting on the book...

Andras,
If you include a section on building and analyzing targeted gene
disruptions, it would be very handy to feature maps of various targeting
constructs which are "out there" in use, for example, pPNT of Tybulewicz et
al. When he was at Lawrence Berkeley Labs, Chris Paszty sent me a full
nucleotide sequence file of pPNT which was very useful in the lab. Some of
the vectors (pKONEO; AF090454) or Stratagene's pKO Scrambler series (e.g.,
AF086845) are accessible from GenBank. These files make the design of
screening primers and prediction of restriction cut sites very easy using a
program like MacVector.

Other vector sequences (such as pPNT) are hard to come by, except in obscure
web sites such as: http://cmmg.biosci.wayne.edu/asg/pPNT

Other vector sequences (such as Richard Palmiter's pNZTK2) are simply
unavailable. Inclusion of vector maps and accession numbers or URLs would
make the 3rd edition more copacetic from the molecular biology end of
targeting.

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From f.lin at victorchang.unsw.edu.au Thu Feb 3 17:25:33 2000
From: f.lin at victorchang.unsw.edu.au (fanglin)
Date: Wed Aug 20 11:55:25 2003
Subject: animals survival and exercise tolerance
Message-ID: <200002030628.RAA22223@gimr.garvan.unsw.edu.au>

Dear All,

We consider to set up animals(mice) survival(natural) and exercise
tolerance experiments. However, I don't any experience on these and would
like to have your suggestions, advice and protocols.

Thanks very much in advance.

Fang

___________________________________________________________________
Fang Lin
Victor Chang Cardiac Research Institute
Garvan Institute of Medical Research
St Vincent's Hospital Tel: (02) 9296 8523
Sydney NSW 2010 Fax: (02) 9295 8101
Australia email: f.lin@victorchang.unsw.edu.au
http://www.victorchang.com.au
___________________________________________________________________

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From k.j.armour at abdn.ac.uk Thu Feb 3 10:09:50 2000
From: k.j.armour at abdn.ac.uk (Kenneth J. Armour)
Date: Wed Aug 20 11:55:25 2003
Subject: 3rd edition of Hogan manual
In-Reply-To: <38988012.501677BD@karmanos.org>
References: <38984CD7.429E0A59@mshri.on.ca>
Message-ID: <l03130301b4bf01f69c6b@[139.133.139.185]>

Andras Nagy wrote:

> I am contributing to the new, third edition of the Brigid Hogan's:
> "Manipulating the Mouse Embryo; A laboratory manual". The plan is to
> make a significant rewriting on the book...

Anton Scott Goustin wrote:

>If you include a section on building and analyzing targeted gene
>disruptions, it would be very handy to feature maps of various targeting
>constructs which are "out there" in use.....
<snip>
> Some of the vectors ..... are accessible from GenBank. These files make
>the design of
>screening primers and prediction of restriction cut sites very easy using a
>program like MacVector.

I've not seen previous editions of the book (we're new to transgenics) but
perhaps some of this information could be provided on a CD-ROM to be
supplied with the book (assuming copyright is not an imutable issue).

Regards

Ken Armour

-o-o-o-o-o-

Kenneth J. Armour Ph.D.
Bone Research Group
Dept. of Medicine and Therapeutics
University of Aberdeen
Polwarth Building
Foresterhill
Aberdeen AB25 2ZD, U.K.

Tel: +44(0)1224 551283
Tel: +44(0)1224 554498/553027 (labs)
Fax: +44 (0) 1224 699884

<mailto: k.j.armour@abdn.ac.uk>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From maria.alexiou at csc.mrc.ac.uk Thu Feb 3 11:17:50 2000
From: maria.alexiou at csc.mrc.ac.uk (Maria Alexiou)
Date: Wed Aug 20 11:55:25 2003
Subject: No subject
Message-ID: <v01540b02b4bf05f6049f@[146.179.68.52]>

>Hello everybody,
>
>I wonder if anyone has done pronuclear injections using eggs from nod mice.
>We have tried here a couple of times using donor mothers which were
>superovulated between 4 and 8 weeks with 5iu PMS/HCG, mated with males
>being 3-4 months old. The females plug and a large number of eggs are
>recovered; the main problem is that the vast majority of them are not
>fertilised and when they are the pronuclei are very tiny to handle. In
>addition, the cytoplasm appears very sparsely granular, and following
>injection the cytoplasm membrane looses its shape.
>Those of you who have worked with them know that these mice are problematic
>since mothers develop diabetes when pregnant. Now, to what extent this is
>relevant to what we see I can not say, but we would be grateful if someone
>having worked with them could help us. There is already a suggestion that
>using F1 eggs from crossing nod mice with bl/6 might overcome the problem.
>True?
>
>Thank you all
>Maria

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Lutz.Bunger at ed.ac.uk Thu Feb 3 13:08:20 2000
From: Lutz.Bunger at ed.ac.uk (Lutz Bunger)
Date: Wed Aug 20 11:55:25 2003
Subject: animals survival and exercise tolerance
In-Reply-To: <200002030628.RAA22223@gimr.garvan.unsw.edu.au>
Message-ID: <200002031308.NAA28153@holyrood.ed.ac.uk>

Dear Fang,
Regarding exercise tolerance we used a tread-mill in mice and have e.g. selected for that performance for quite a while (50 and more
generations).
We called the trait endurance fitness and several papers are published about it, some of them are in German but some are in English.
You might want to browse trough the references linked to our website, you will find in my signature.
Hope this helps.
Regards,
Lutz

Date sent: Thu, 3 Feb 2000 17:25:33 +1000
To: transgenic-list@ic.ac.uk
From: f.lin@victorchang.unsw.edu.au (fanglin)
Subject: animals survival and exercise tolerance
Send reply to: f.lin@victorchang.unsw.edu.au (fanglin)

> Dear All,
>
> We consider to set up animals(mice) survival(natural) and exercise
> tolerance experiments. However, I don't any experience on these and would
> like to have your suggestions, advice and protocols.
>
> Thanks very much in advance.
>
> Fang
>
> ___________________________________________________________________
> Fang Lin
> Victor Chang Cardiac Research Institute
> Garvan Institute of Medical Research
> St Vincent's Hospital Tel: (02) 9296 8523
> Sydney NSW 2010 Fax: (02) 9295 8101
> Australia email: f.lin@victorchang.unsw.edu.au
> http://www.victorchang.com.au
> ___________________________________________________________________
>
>
>
>
> "transgenic-list" web site (archives, FAQs, protocols, links) address:
> http://www.med.ic.ac.uk/db/dbbm/tglist.htm

Lutz Bunger
ICAPB, Kings Buildings
The University of Edinburgh
Edinburgh EH9 3JT
Scotland

Tel 44 (0)131 650 5442
Fax 44 (0)131 667 3210
Email Lutz.Bunger@ed.ac.uk
http://www.ed.ac.uk/~eang17/mice.html
http://helios.bto.ed.ac.uk/icapb/staff/frames.html

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From mmichalkiewicz at hsc.wvu.edu Thu Feb 3 10:28:17 2000
From: mmichalkiewicz at hsc.wvu.edu (Mike Michalkiewicz)
Date: Wed Aug 20 11:55:25 2003
Subject: your input is needed
Message-ID: <s8995860.003@mail.hsc.wvu.edu>

Andras,
here are my little 3 suggestions:
1. Include a protocol on transgenic rat production. We could provide you with the protocol we have developed here and are happy with.
2. Include a protocol on transgenic rodents production using YAC clones (e.g., large DNA sizes, 300-500kb)
3. If you need to shorten any part: do it with the "Mouse development" section. Although it is an excellent one and I have learnt a lot from it, is probably practically not used very often.

Regards,

Mike Michalkiewicz, DVM, PhD

West Virginia University
Department of Physiology
PO Box 9229
Morgantown, WV 26506
phone 304 293 1515; Fax 304 293 3850
http://www.hsc.wvu.edu/neurosci/faculty/michalkiewicz.html

>>> Andras Nagy <nagy@mshri.on.ca> 02/02/00 10:27AM >>>
Dear List,

Thanks very much for your contribution, which will be properly
acknowledged.

Andras Nagy, PhD
Senior Staff Scientist
Mount Sinai Hospital
Samuel Lunenfeld Research Institute
600 University Ave.
Toronto, M5G 1X5
Canada

Email: nagy@mshri.on.ca
tel. (416)-586-3246 0ffice
tel. (416)-586-8455 Lab
tel. (416)-586-5361 Secretary
fax (416)-586-8588
URL: http://www.mshri.on.ca/nagy/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From pasceri at sickkids.on.ca Thu Feb 3 11:42:48 2000
From: pasceri at sickkids.on.ca (Peter Pasceri)
Date: Wed Aug 20 11:55:25 2003
Subject: NOD mice
Message-ID: <l03130301b4bf5b0b1e6a@[142.20.155.242]>

Hi Maria.
You pretty well summed up the problems with NODS. When I must inject into
NODS I usually give 2.5 iu of hormones. One thing I've never done is
titrate the levels of hormones given. I suspect the level of PMS probably
affects the quality and quantity of eggs. Egg numbers is not a problem
with yeilds in excess of 400. The problem is getting good injectable eggs.
The PMS level is probably too high. If you plan on doing a lot of NOD
injections hormone titration may be one thing to consider. When the nuclei
are too small I usually let the eggs sit for a couple of hours so that the
nuclei increase in size. Never tried crossing to bl/6 since we want a pure
NOD background. My day injecting NODS is usually very frustrating and
difficult but I have had some success.

Good luck.
Peter

--
Peter Pasceri
Program of Developmental Biology
The Hospital for Sick Children
555 University Avenue
Toronto, Ontario, Canada, M5G-1X8

Phone: ( 416 ) 813-8169
Fax: ( 416 ) 813-8883
E-mail: pasceri@sickkids.on.ca

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jscott at wehi.EDU.AU Fri Feb 4 08:17:15 2000
From: jscott at wehi.EDU.AU (Julie Scott)
Date: Wed Aug 20 11:55:25 2003
Subject: your mail
In-Reply-To: <v01540b02b4bf05f6049f@[146.179.68.52]>
Message-ID: <Pine.GSO.4.05.10002040747190.23343-100000@wehiz.wehi.EDU.AU>

Hi Maria,
I have been injecting nod eggs for some time now and I agree they are not
very user friendly. I have observed the same things that you have
described.
I superovulate 8 week old females with 5iu PMS/HCG, I usually recover
large numbers of eggs with about 50% to sometimes 70% fertile. I harvest
the eggs at 12pm and check for fertility at about 2pm and I never start
injecting before 4pm. When I started checking fertility later in the day I
noticed an increase in the number of fertile eggs, I guess this maybe due
to the pronuclei being so small earlier that they didn't look fertile and
I was throwing them out. By 4pm the majority of eggs have a nice pronuclei
for injection although they are still very fragile.
I hope this has helped,
Bye for now, Julie.

On Thu, 3 Feb 2000, Maria Alexiou wrote:

> >Hello everybody,
> >
> >I wonder if anyone has done pronuclear injections using eggs from nod mice.
> >We have tried here a couple of times using donor mothers which were
> >superovulated between 4 and 8 weeks with 5iu PMS/HCG, mated with males
> >being 3-4 months old. The females plug and a large number of eggs are
> >recovered; the main problem is that the vast majority of them are not
> >fertilised and when they are the pronuclei are very tiny to handle. In
> >addition, the cytoplasm appears very sparsely granular, and following
> >injection the cytoplasm membrane looses its shape.
> >Those of you who have worked with them know that these mice are problematic
> >since mothers develop diabetes when pregnant. Now, to what extent this is
> >relevant to what we see I can not say, but we would be grateful if someone
> >having worked with them could help us. There is already a suggestion that
> >using F1 eggs from crossing nod mice with bl/6 might overcome the problem.
> >True?
> >
> >Thank you all
> >Maria
>
>
>
> "transgenic-list" web site (archives, FAQs, protocols, links) address:
> http://www.med.ic.ac.uk/db/dbbm/tglist.htm
>
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From saboulard at hotmail.com Fri Feb 4 11:29:22 2000
From: saboulard at hotmail.com (didier SABOULARD)
Date: Wed Aug 20 11:55:25 2003
Subject: HPV-16 / GM-CSF
Message-ID: <20000204102922.69110.qmail@hotmail.com>

Dear All,

We consider to set up animals experiments and we need transgenic mice : the
first one with human papillomavirus 16 (HPV16) and the second one GM-CSF
deficient (granulocyte/macrophage colony-stimulating factor). I would like
to have your suggestions and advice to find these two stocks of mice.
Thanks very much in advance.

Dr DIDIER SABOULARD
LABORATORY OF PATHOLOGY
CHU Liege, Belgium
saboulard@hotmail.com
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From parker-thornburg.1 at osu.edu Fri Feb 4 14:44:13 2000
From: parker-thornburg.1 at osu.edu (Jan Parker-Thornburg)
Date: Wed Aug 20 11:55:25 2003
Subject: swimming mice
Message-ID: <v04210102b4c0fe16234d@[128.146.132.236]>

No, we aren't having a problem with the auto-watering system. I have
a PI who actually wants to train her mice to swim for the scientific
data it will provide. She asked me a number of questions about it,
and I told her that I would check with this group. My experience
with swimming rodents (other than failures of the auto-watering
system) were a large number of years ago with hamsters. I hope that
someone out there knows of more up-to-date protocols. Perhaps the
best thing to do is if you know of someone who has used swimming as
part of their analysis, to send the e-mail address directly to the
PI, or give her e-mail to that person (hai.2@osu.edu). That way,
they can "talk amongst themselves" and she can get answers to her
questions directly. Let me know what you come up with. And, thanks.

Jan
Jan Parker-Thornburg, PhD
Manager, Transgenic Animal Facility
066 Rightmire Hall
1060 Carmack Rd.
The Ohio State University
Columbus, OH 43210
Phone: (614) 292-8715
Fax: (614) 292-5379
parker-thornburg.1@osu.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Linda.Watkins at stjude.org Fri Feb 4 14:00:40 2000
From: Linda.Watkins at stjude.org (Watkins, Linda )
Date: Wed Aug 20 11:55:25 2003
Subject: caspase 8 mice
Message-ID: <83B4CF76D818D111982000805F31CA5302D144A8@sjmemexc1.stjude.org>

Dear List Members,

I have been tasked to find a CASPASE 8 mouse source... anyone knowing of
such a source for this mouse, please contact me... I would surely appreciate
it......

Linda Watkins

Linda Watkins, LATg
Animal Procurement Coordinator
St. Jude Children's Research Hospital
Animal Resources Center
332 North Lauderdale
Memphis, TN 38105
901-495-3385 or 901-495-2956 (office)
901-495-3112 (fax)
linda.watkins@stjude.org <mailto:linda.watkins@stjude.org>
http://www.stjude.org

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From szhao at bcm.tmc.edu Fri Feb 4 14:05:45 2000
From: szhao at bcm.tmc.edu (Shulei Zhao)
Date: Wed Aug 20 11:55:25 2003
Subject: promoter for targeting choroid plexus
Message-ID: <Pine.GSO.4.10.10002041404560.23365-100000@watson.bcm.tmc.edu>

I would like to know whether there are promoters that can be used to
target transgene expression to immature choroid plexus or its
precursor. The promoter does not have to be completely choroid plexus
specific. Thanks.

Shulei Zhao
Dept of Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX 77030

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Fri Feb 4 18:13:44 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Wed Aug 20 11:55:25 2003
Subject: Hogan III and NT technology
References: <38984CD7.429E0A59@mshri.on.ca>
Message-ID: <389B5D27.6F82724F@karmanos.org>

Andras Nagy wrote:

> ...the new, third edition of the Brigid Hogan's, "Manipulating the Mouse
> Embryo; A laboratory manual". The plan is to make a significant rewriting
> on the book, which will reflect the changes occurred in the last six years
> concerning the transgenic technology of the mouse.
>
> "transgenic-list" web site (archives, FAQs, protocols, links) address:
> http://www.med.ic.ac.uk/db/dbbm/tglist.htm

Perhaps one of the biggest "stories" in the experimental embryology of the
mouse in recent years is cloning using nuclear transplantation (NT), first
done in frogs by Briggs & King in the 1950, but not achieved in mammals
until the Roslin Institute group made Dolly.

The technology for NT in mice is not trivial. The original reports on
successful NT in the mouse in the 1970s turned out to be unreproducible.
The Hawaiian group of Teruhiko Wakayama and Ryuzo Yanagimachi have published
a number of reports in the last two years using donor nuclei from cells of
the cumulus oophorus (Wakayama et al., Nature 394, 369 (1998)), fibroblastic
cells from adult tail tips (Nature Genetics 22, 127 (1999)), and more
recently using nuclei taken from embryonic stem (ES) cells (Wakayama et al.,
PNAS 96, 14984 (1999)).

The Hawaiian group uses some bizarre (to me) approaches like piezo-electric
driven microinjection, culture of the injected oocytes in medium containing
strontium ions, and so on. In their most recent paper, it looks as if some
ES cell lines are pretty good for NT (e.g., R1) and others are pretty lousy
(E14, from 129/Ola mice). All the mice born in this paper (31 live mice out
of 2852 enucleated oocytes injected = ~1%) are male, of course, and
basically wt but they suggest that it may be possible to use ES clones which
have gone through targeted (homologous) recombination events as well (but
the one line they tried failed to produce a live-born mouse).

It would be wonderful to see this technology "catch on", and Hogan III could
certainly catalyze this technology spread. I would love to see a chapter
devoted to NT.

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From milstone at rascal.med.harvard.edu Sat Feb 5 22:10:11 2000
From: milstone at rascal.med.harvard.edu (David Milstone)
Date: Wed Aug 20 11:55:25 2003
Subject: promoter for targeting choroid plexus
In-Reply-To: <E12Gp8D-0005HG-00@tomatin.cc.ic.ac.uk>
References: <E12Gp8D-0005HG-00@tomatin.cc.ic.ac.uk>
Message-ID: <v0420550ab4c293da97c9@[134.174.88.145]>

My impression from casual conversations is that *many* transgenes are
expressed in choroid plexus, as if it were "the testis of the CNS".

>Date: Fri, 4 Feb 2000 14:05:45 -0600 (CST)
>From: Shulei Zhao <szhao@bcm.tmc.edu>
>Subject: promoter for targeting choroid plexus
>
>I would like to know whether there are promoters that can be used to
>target transgene expression to immature choroid plexus or its
>precursor. The promoter does not have to be completely choroid plexus
>specific. Thanks.
>
>
>Shulei Zhao
>Dept of Molecular and Cellular Biology
>Baylor College of Medicine
>Houston, TX 77030
>

*******************************************************************
David S. Milstone, M.D., Ph.D. LMRC 421
Vascular Research Division 221 Longwood Ave
Department of Pathology Boston, MA 02115
Brigham & Women's Hospital milstone@rascal.med.harvard.edu
Harvard Medical School Tel: (617) 278-0173
LMRC 421
221 Longwood Ave
Boston, MA 02115
e-mail milstone@rascal.med.harvard.edu
Tel: (617) 278-0173 Office
Tel: (617) 278-0195 Lab
FAX: (617) 975-0896
*******************************************************************

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Steven.Sansing at bms.com Mon Feb 7 08:10:27 2000
From: Steven.Sansing at bms.com (Steven R Sansing)
Date: Wed Aug 20 11:55:25 2003
Subject: non-transgenic animals
Message-ID: <389EC443.377D2FD8@bms.com>

Recently our veterinarians had attended a meeting and one of the
topics that was discussed regarded the "non-use" of negative
transgenic animals. I'm interested to find out how others are
dealing w/ these animals? If there's enough responses i'll
summarize a list for the group. Thanks in advance....

Steve Sansing
Bristol-Myers Squibb
Wallingford, Ct
steven.sansing@bms.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Steven.Sansing at bms.com Mon Feb 7 09:03:18 2000
From: Steven.Sansing at bms.com (Steven R Sansing)
Date: Wed Aug 20 11:55:25 2003
Subject: non-transgenic use
Message-ID: <389ED0A6.55A0B14B@bms.com>

All,

I've started receiving some responses and it appears that there's
some confusion over what i'm asking. Hopefully this will help.

Are people using their "negative" animals? (either transgenic or
knockout). OR are the animals always euthanatized?

IF people were requested to use the "negative" animals what would
be some concerns?

Again i hope this clears up some of the confusion and thanks
again....

Steve Sansing
Bristol-Myers Squibb
Wallingford, CT
steven.sansing@bms.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From pozzi at crs.ifo.it Tue Feb 8 12:11:21 2000
From: pozzi at crs.ifo.it (Laura Pozzi)
Date: Wed Aug 20 11:55:25 2003
Subject: No subject
Message-ID: <3.0.1.32.20000208121121.0068fc5c@crs.ifo.it>

To the KO-logists community,
we are stuck with a
targeting vector that yields very low numbers of G418 resistant clones
/electroporation. The clones are < 50 / 10 million electroporated cells.
The neo cassette in our construct is loxed: could this be a reason? has
anyone had the same problem? does anyone have some other possible reason?
With other non loxed constructs we always had >100 clones / electroporation.
Thank you in advance for any reply

Laura
Dr.Laura Pozzi
Lab.Modelli Animali
Centro Ricerca Sperimentale
Istituto Regina Elena
Via delle Messi d'oro 156
00158 Roma, Italy
tel. -39 06 49852512
fax - 39 06 49852505
email pozzi@crs.ifo.it

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From elena.notarianni at ncl.ac.uk Tue Feb 8 11:48:48 2000
From: elena.notarianni at ncl.ac.uk (Elena Notarianni)
Date: Wed Aug 20 11:55:25 2003
Subject: No subject
Message-ID: <200002081147.LAA20031@cheviot1.ncl.ac.uk>

Dear List,

Does anyone know of a source of STO cells with an FGF2 knock-out?
Thanks, Elena.

Dr. Elena Notarianni,
Comparative Biology Centre,
The Medical School,
Newcastle University,
Framlington Place,
Newcastle Upon Tyne NE2 4HH.
Tel: 0191 222 8817 or 6715
FAX: 0191 222 8688.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From sosuka at mr-envi.med.osaka-u.ac.jp Tue Feb 8 21:03:59 2000
From: sosuka at mr-envi.med.osaka-u.ac.jp (Soh F. Osuka)
Date: Wed Aug 20 11:55:25 2003
Subject: To the KO-logists community,
In-Reply-To: <3.0.1.32.20000208121121.0068fc5c@crs.ifo.it>
Message-ID: <200002081223.VAA18848@mr-envi.med.osaka-u.ac.jp>

Dear Laura

What is the source of neo gene?

In my experience, I got a very low number of G418 resistant colonies when I
use mutated neo from pPNT to target one gene, while the other lab member is
OK for other targeting with it.

After a replacement of mutated neo with wild-type one from
pMC1neo(Stratagene) in the targeting vector, the problem was solved.

Thanks,

Soh

At 0:11 PM +0100 00.2.8, Laura Pozzi wrote:
>To the KO-logists community,
> we are stuck with a
>targeting vector that yields very low numbers of G418 resistant clones
>/electroporation. The clones are < 50 / 10 million electroporated cells.
>The neo cassette in our construct is loxed: could this be a reason? has
>anyone had the same problem? does anyone have some other possible reason?
>With other non loxed constructs we always had >100 clones / electroporation.

_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
Soh F. Osuka, M.Sc.
Lab. for Learning & Memory, RIKEN Brain Science Institute(JAPAN)
*Now please contact to;
Dep. of Enviromental Medicine(H3), Osaka Univ. Medical School(JAPAN)
tel+fax:+81-6-6879-3266 e-mail:sosuka@mr-envi.med.osaka-u.ac.jp
_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Biborka.BVeress at cmb.ki.se Tue Feb 8 15:26:50 2000
From: Biborka.BVeress at cmb.ki.se (Biborka Bereczky Veress , Genetik)
Date: Wed Aug 20 11:55:25 2003
Subject: Hogan III and NT technology
Message-ID: <l03110704b4c5d67046e4@[130.237.120.40]>

>>Date: Fri, 04 Feb 2000 18:13:44 -0500
>>From: Anton Scott Goustin <goustina@karmanos.org>
>>Organization: Karmanos Cancer Institute
>>X-Accept-Language: en
>>MIME-Version: 1.0
>>To: Andras Nagy <nagy@mshri.on.ca>
>>CC: transgenic list <transgenic-list@ic.ac.uk>
>>Subject: Hogan III and NT technology
>>Sender: owner-transgenic-list@ic.ac.uk
>>Precedence: bulk
>>Reply-To: Anton Scott Goustin <goustina@karmanos.org>
>>
>>Andras Nagy wrote:
>>
>>> ...the new, third edition of the Brigid Hogan's, "Manipulating the Mouse
>>> Embryo; A laboratory manual". The plan is to make a significant rewriting
>>> on the book, which will reflect the changes occurred in the last six years
>>> concerning the transgenic technology of the mouse.
>>>
>>> "transgenic-list" web site (archives, FAQs, protocols, links) address:
>>> http://www.med.ic.ac.uk/db/dbbm/tglist.htm
>>
>>Perhaps one of the biggest "stories" in the experimental embryology of the
>>mouse in recent years is cloning using nuclear transplantation (NT), first
>>done in frogs by Briggs & King in the 1950, but not achieved in mammals
>>until the Roslin Institute group made Dolly.
>>
>>The technology for NT in mice is not trivial. The original reports on
>>successful NT in the mouse in the 1970s turned out to be unreproducible.
>>The Hawaiian group of Teruhiko Wakayama and Ryuzo Yanagimachi have published
>>a number of reports in the last two years using donor nuclei from cells of
>>the cumulus oophorus (Wakayama et al., Nature 394, 369 (1998)), fibroblastic
>>cells from adult tail tips (Nature Genetics 22, 127 (1999)), and more
>>recently using nuclei taken from embryonic stem (ES) cells (Wakayama et al.,
>>PNAS 96, 14984 (1999)).
>>
>>The Hawaiian group uses some bizarre (to me) approaches like piezo-electric
>>driven microinjection, culture of the injected oocytes in medium containing
>>strontium ions, and so on. In their most recent paper, it looks as if some
>>ES cell lines are pretty good for NT (e.g., R1) and others are pretty lousy
>>(E14, from 129/Ola mice). All the mice born in this paper (31 live mice out
>>of 2852 enucleated oocytes injected = ~1%) are male, of course, and
>>basically wt but they suggest that it may be possible to use ES clones which
>>have gone through targeted (homologous) recombination events as well (but
>>the one line they tried failed to produce a live-born mouse).
>>
>>It would be wonderful to see this technology "catch on", and Hogan III could
>>certainly catalyze this technology spread. I would love to see a chapter
>>devoted to NT.
>>
>>* * * * * * * * * * * * * * * * * *
>>Anton-Scott Goustin, Ph.D.
>>Assistant Professor
>>Program in Molecular Biology and Genetics
>>Karmanos Cancer Institute
>>110 East Warren (Prentis 311)
>>Detroit, Michigan USA 48201-1379
>>PHONE 313-833-0715 x2384
>>FAX 313-832-7294
>>* * * * * * * * * * * * * * * * * *
>>
>>
>>Content-Type: text/x-vcard; charset=us-ascii;
>> name="goustina.vcf"
>>Content-Transfer-Encoding: 7bit
>>Content-Description: Card for Anton Scott Goustin
>>Content-Disposition: attachment;
>> filename="goustina.vcf"
>>
>>Attachment converted: Macintosh HD:goustina.vcf 4 (TEXT/ttxt) (0000A353)

Thank you, Anton-Scott Goustin, for your suggestion! I also planned to
point out the importance of this field and the interest it would meet.
Taking in consideration that probably there is a long time to come until
the Hogan IV edition comes out, it really seems to be necessary to include
a chapter on this hot NT topic.

************************************************************

Biborka Bereczky Veress
research engineer
Karolinska Institutet
CMB/MouseCamp
Von Eulers v?g 3
171 77 Stockholm

Tel. office: + 46 8 728 73 26
Tel. lab.: + 46 8 728 76 13, 75 14
Fax: + 46 8 348135
e-mail: Biborka.BVeress@cmb.ki.se

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From junming at physio1.utmem.edu Tue Feb 8 20:45:46 2000
From: junming at physio1.utmem.edu (Junming Yue)
Date: Wed Aug 20 11:55:25 2003
Subject: EGFP
Message-ID: <l03130301b4c676f88929@[132.192.44.66]>

Dear Dieter,

Could you send me your mail address again?

Junming Yue

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tjf at uci.edu Tue Feb 8 09:06:03 2000
From: tjf at uci.edu (Tom Fielder)
Date: Wed Aug 20 11:55:25 2003
Subject: cryopreservation fees survey
Message-ID: <v04003a06b4c58bd8db0e@[128.200.21.145]>

Hi all,

I've had about 5 responses to this survey so far. It's been a month since
I first posted it, so I'm sending it again in hopes of getting a larger
sample size before posting a summary. Here it is again:

What do you charge for freezing 8-cell stage mouse embryos?

How many donors and/or studs do you ask the client to supply?

How do you recover the storage costs (e.g., liquid nitrogen, labor, etc.) -
is this billed on a yearly basis, or do you charge it as one lump sum up
front?

What do you charge for reconstituting a frozen line? Is this billed as a
separate service?

Do your fees vary depending on the strain of mouse?

Thanks in advance.

Tom

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

************************************************************

Biborka Bereczky Veress
research engineer
Karolinska Institutet
CMB/MouseCamp
Von Eulers v?g 3
171 77 Stockholm

Tel. office: + 46 8 728 73 26
Tel. lab.: + 46 8 728 76 13, 75 14
Fax: + 46 8 348135
e-mail: Biborka.BVeress@cmb.ki.se

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

Hi all,

What do you charge for freezing 8-cell stage mouse embryos?

How many donors and/or studs do you ask the client to supply?

How do you recover the storage costs (e.g., liquid nitrogen, labor, etc.) -
is this billed on a yearly basis, or do you charge it as one lump sum up
front?

What do you charge for reconstituting a frozen line? Is this billed as a
separate service?

Do your fees vary depending on the strain of mouse?

Thanks in advance.

Tom

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

Dear List,

Does anyone know of a source of STO cells with an FGF2 knock-out?
Thanks, Elena.

Dr. Elena Notarianni,
Comparative Biology Centre,
The Medical School,
Newcastle University,
Framlington Place,
Newcastle Upon Tyne NE2 4HH.
Tel: 0191 222 8817 or 6715
FAX: 0191 222 8688.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From pozzi at crs.ifo.it Tue Feb 8 12:11:21 2000
From: pozzi at crs.ifo.it (Laura Pozzi)
Date: Wed Aug 20 11:55:25 2003
Subject: No subject
Message-ID: <3.0.1.32.20000208121121.0068fc5c@crs.ifo.it>

Laura
Dr.Laura Pozzi
Lab.Modelli Animali
Centro Ricerca Sperimentale
Istituto Regina Elena
Via delle Messi d'oro 156
00158 Roma, Italy
tel. -39 06 49852512
fax - 39 06 49852505
email pozzi@crs.ifo.it

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

Dear Dieter,

Could you send me your mail address again?

Junming Yue

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

Dear Laura

What is the source of neo gene?

In my experience, I got a very low number of G418 resistant colonies when I
use mutated neo from pPNT to target one gene, while the other lab member is
OK for other targeting with it.

After a replacement of mutated neo with wild-type one from
pMC1neo(Stratagene) in the targeting vector, the problem was solved.

Thanks,

Soh

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Andrew.Davidson at med.monash.edu.au Wed Feb 9 11:48:22 2000
From: Andrew.Davidson at med.monash.edu.au (DR AD DAVIDSON [MIC])
Date: Wed Aug 20 11:55:25 2003
Subject: effects of duplicated vector sequences on integration
Message-ID: <CCBBBE737F@its-med.cc.monash.edu.au>

Hi all,

I have a question regarding the effects of duplicated vector
sequences on transgene integration.

I have produced a construct which expresses two genes
bi-directionally under control of the tTA/rTA regulated promoter
(using the Clontech tet vector pBI). To achieve optimal
transient expression levels I have had to engineer a
beta globin intron 5 prime to both of the genes.

As the intron is now duplicated in the construct (on opposite sides
of the promoter) I am wondering what effect this may have on
integration of the whole cassette when producing animals by
pronuclear injection.

thanks in advance,

Andrew
* Dr Andrew Davidson *
* Department of Microbiology, *
* Monash University, *
* P.O. Box 53, Clayton, 3800 *
* Australia *
* phone +61 03 99054814 *
* fax +61 03 99054811 *
* Andrew.Davidson@med.monash.edu.au *

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From icta2000 at hotmail.com Wed Feb 9 12:24:35 2000
From: icta2000 at hotmail.com (ICTA 2000)
Date: Wed Aug 20 11:55:25 2003
Subject: 3rd ICTA.
Message-ID: <20000209122435.36296.qmail@hotmail.com>

3rd International Conference on Transgenic Animals(ICTA)
(with biotech exhibition)
Oct.16-21, 2000 Beijing China

Sponsor:

ISTR(International Society for Transgenic Research)

Co-Sponsors:

Transgenic Research
transgenic-list

Co-chairs: David L. Kooyman <david_kooyman@byu.edu>
Eckhard Wolf <ewolf@lmb.uni-muenchen.de>

Keynote speaker:

Vern Pursel, PhD (USDA, USA)

Invited Speakers:

Carl Pinkert, PhD(UAB, USA)
Robert Ivarie, PhD(Univ of Georgia, Athens, GA, USA)
Ignacio Anegon, MD(Sante University, Nantes, France)
Georg Arnold, PhD(Gene Center, LMU, Munich, Germany)
Rathin Das, PhD (American Biotechnology Laboratory,
USA)
Ilya Raskin, PhD (Rutgers University, New Brunswick,
NJ, USA)

Scientific Sessions:

1. Transgenic expression in mammals
Peter Sobieszczuk, PhD chair (Imperial College of
Medicine, London, UK)

2. Transgenic expression in plants
Ilya Raskin, PhD chair (Rutgers University, New
Brunswick, NJ, USA)

3. Transgenic Disease models
Jing Zhou, MD, PhD chair (Harvard Inst. Med, Boston,
Mass., USA)

4. Transgenic expression in poultry
Robert Ivarie, PhD chair (Univ of Georgia, Athens, GA,
USA)

5. Transgenic expression in fish
Richard Winn, PhD chair (Univ of Georgia, Athens, GA,
USA)

6. Transgenic transplantation models
Ignacio Anegon, MD chair (Sante University, Nantes,
France)

7. Transgenic Techniques
Mike Martin, PhD chair (Nextran, Princeton, NJ, USA)

8. Manipulation of ES cells and Cloning
Jerry Yang, PhD chair (Univ. of Conn. Storrs, CT, USA)

9. Genomic and Proteomic analysis of transgenic animal
models-Technical Session
Georg Arnold, PhD chair (Gene Center, LMU, Munich, Germany)

10. ENU Mutagenesis-phenotype driven approaches for
analyzing gene function-Technical Session
Eckhard Wolf, PhD chair (Inst. of Molecular Animal
Breeding, LMU, Munich, Germany)

Supporters:

American Biotechnology Laboratory (a publication of
International Scientific Communications, Inc., USA)
PHI(USA);
Kluwer Academic Publishers(Netherlands);
Miniplast(Israel);
BIO 101, Inc.(USA)
(continued)

Organizers:

BILONG Transgenics;
CICCST(China International Conference Center for Science & Technology)

Abstract Submission:

The abstracts of the 3rd ICTA will be published on
Transgenic Research. An electronic version for each abstract must be
sent
to:

david_kooyman@byu.edu

before it is mailed to:

David L. Kooyman, PhD
Department of Animal and Veterinary Science
Brigham Young University
392 WIDB, Provo
UT 84602-5169
USA

Abstract Deadline: May 1, 2000

General Contact:

Ms. Peifeng Chen, Administrative Coordinator
Ms. Jenny Zuo, Local Coordinator

BILONG Transgenics
Add: C13 Beiyitiao, Zhongguancun, Beijing 100080, China
Box: P.O. Box 2714, Beijing 100080, China
Tel: 86-10-8262-5664
Fax: 86-10-6253-2114
Email: 3rdICTA@bilong.com
Web: www.ciccst.org.cn/icta

______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From ekothari at biomail.ucsd.edu Wed Feb 9 12:53:55 2000
From: ekothari at biomail.ucsd.edu (Ella Kothari)
Date: Wed Aug 20 11:55:26 2003
Subject: Fwd: Re:
Message-ID: <v04003a06b4c77634c861@[132.239.200.182]>

>Date: Wed, 9 Feb 2000 12:43:25 -0700
>To: maria.alexiou@csc.mrc.ac.uk (Maria Alexiou)
>From: Ella Kothari <ekothari@biomail.ucsd.edu>
>Subject: Re:
>Cc:
>Bcc:
>X-Attachments:
>
>>>Hello everybody,
>>>
>>>I wonder if anyone has done pronuclear injections using eggs from nod mice.
>>>We have tried here a couple of times using donor mothers which were
>>>superovulated between 4 and 8 weeks with 5iu PMS/HCG, mated with males
>>>being 3-4 months old. The females plug and a large number of eggs are
>>>recovered; the main problem is that the vast majority of them are not
>>>fertilised and when they are the pronuclei are very tiny to handle. In
>>>addition, the cytoplasm appears very sparsely granular, and following
>>>injection the cytoplasm membrane looses its shape.
>>>Those of you who have worked with them know that these mice are problematic
>>>since mothers develop diabetes when pregnant. Now, to what extent this is
>>>relevant to what we see I can not say, but we would be grateful if someone
>>>having worked with them could help us. There is already a suggestion that
>>>using F1 eggs from crossing nod mice with bl/6 might overcome the problem.
>>>True?
>>>
>>>Thank you all
>>>Maria
>>
>>
>>
>>"transgenic-list" web site (archives, FAQs, protocols, links) address:
>>http://www.med.ic.ac.uk/db/dbbm/tglist.htm
>
>Hi Maria,
> We've worked with NODs and always found that the females were only
>superovulatable at 12 weeks of age or older.At this age they 've given us
>great yields of eggs and were very easy to inject too in terms of large
>targets to inject etc.
> Goodluck.
> Ella.
>
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From dee at mri8.mri.montana.edu Wed Feb 9 13:53:56 2000
From: dee at mri8.mri.montana.edu (Dee Goss)
Date: Wed Aug 20 11:55:26 2003
Subject: gas chamber for IVF
Message-ID: <01bf733f$c840ff60$2a54c8cc@dee.po.mri.montana.edu>

Hi,
Does anyone know were I can get a modular incubator chamber? Some call it a satellite.

Thank You,
Dee Goss
Transgene Facility
McLaughlin Research Institute
1520 23rd Str. S.
Great Falls, Mt. 59404
dee@po.mri.montana.edu
(406)452-6208
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From jdl at po.cwru.edu Wed Feb 9 16:29:15 2000
From: jdl at po.cwru.edu (Jackie Nicholson)
Date: Wed Aug 20 11:55:26 2003
Subject: gas chamber for IVF
Message-ID: <l03130314b4c78c5f3cae@[129.22.89.103]>

Modular incubator chamber from Billups-Rothenburg
Box 977
Del Mar, CA 92014

visit genesis: The Journal of Genetics and Development
http://www.wiley.com/genesis

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From German.Vergara at wl.com Wed Feb 9 17:59:22 2000
From: German.Vergara at wl.com (Vergara, German)
Date: Wed Aug 20 11:55:26 2003
Subject: FW: gas chamber for IVF
Message-ID: <91DF46AF9D52D31191D600805FBEC08D6F8F73@wolf.research.aa.wl.com>

-----Original Message-----
From: Vergara, German
Sent: Wednesday, February 09, 2000 1:15 PM
To: 'Dee Goss'
Subject: RE: gas chamber for IVF

You can get one from billups-Rothenberg, Inc
Tel: 619-755-3309
Modular Incubator Chamber MIC-101 They were $379

-----Original Message-----
From: Dee Goss [mailto:dee@mri8.mri.montana.edu]
Sent: Wednesday, February 09, 2000 12:54 PM
To: transgenic-list@ic.ac.uk
Subject: gas chamber for IVF

Hi,
Does anyone know were I can get a modular incubator chamber? Some call it
a satellite.

Thank You,
Dee Goss
Transgene Facility
McLaughlin Research Institute
1520 23rd Str. S.
Great Falls, Mt. 59404
dee@po.mri.montana.edu <mailto:dee@po.mri.montana.edu>
(406)452-6208

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tjf at uci.edu Thu Feb 10 15:42:10 2000
From: tjf at uci.edu (Tom Fielder)
Date: Wed Aug 20 11:55:26 2003
Subject: BUB/BnJ superovulation
Message-ID: <v04003a0cb4c88bad5b91@[128.200.21.145]>

Does anyone have experience with superovulating female mice of the BUB/BnJ
strain for DNA microinjection? If so, what kind of egg yields did you get,
and were there any unusual problems involved? Also, what age femlaes did
you use?

thanks in advance,
Tom

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Steven.Sansing at bms.com Fri Feb 11 10:32:24 2000
From: Steven.Sansing at bms.com (Steven R Sansing)
Date: Wed Aug 20 11:55:26 2003
Subject: non transgenic animal use
Message-ID: <38A42B88.DEC1B46C@bms.com>

Thanks for all the responses regarding my question of use of
"non-transgenic","wildtype" animals: This is a breakdown of the
13 responses i received:

Over 98% of the people save some as controls and euthanatize the
rest.

Other possible uses by some investigators were:
Vasectomize some males
Use females as pseudopregnant
Use females as foster moms
Use for teaching. (i.e. practice egg collections, harvest,
transfer)
Possible donation to Zoo

The rational for not using the wildtype animals were:
A concern that there possibly would be a modification of the
genome which didn't result in integration.
Possibly there was integration in such a way that the screening
procedures couldn't detect.
A response from the UK says that these animals have been
classified as "having been through a procedure" (breeding), so
these animals can't be used unless they receive permission from
the home office.

Steve Sansing
Bristol-Myers Squibb
Wallingford, CT
steven.sansing@bms.com
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From Donna.ROBERTS at imvs.sa.gov.au Mon Feb 14 15:17:27 2000
From: Donna.ROBERTS at imvs.sa.gov.au (Donna Roberts)
Date: Wed Aug 20 11:55:26 2003
Subject: Homozygous transgenics lines
Message-ID: <3.0.6.32.20000214151727.00793100@mail.imvs.sa.gov.au>

Hi all,

My boss has this "thing" about making our transgenic lines homozygous -
personally I don't think it's worth it, because most of the breeding is
only done to maintain the lines.

We basically have two cages of males for each line (approx 50 cages) and
only breed them when he goes "oh crap, they're 10 months old, we'd better
breed them or we'll lose the line!"

As I say I can't see the point in breeding to obtain homozygous lines,
which would take two or three generations to confirm, and generate heaps of
cages, when three generations, as we do at the moment, would be bred over
about 2 1/2 - 3 years.

Any ideas for or against would be much appreciated.

Thanks is advance

Donna Roberts
Transgenic Mouse Facility
Hanson Centre for Cancer Research
Institute of Medical and Veterinary Science
Frome Road
Adelaide 5000
South Australia
Ph: +61 8 8222 3473
Fax: +61 8 8232 4092
Email: donna.roberts@imvs.sa.gov.au

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From clandel at jax.org Mon Feb 14 09:58:25 2000
From: clandel at jax.org (Carlisle Landel)
Date: Wed Aug 20 11:55:26 2003
Subject: Homozygous transgenics lines
In-Reply-To: <3.0.6.32.20000214151727.00793100@mail.imvs.sa.gov.au>
References: <3.0.6.32.20000214151727.00793100@mail.imvs.sa.gov.au>
Message-ID: <v04210102b4cdc6e16c3a@[199.94.155.139]>

Pro:

1. If you are going to maintain this line, it is much easier to do
as homozygotes. You won't have to type offspring.

2. You might find some really cool phenotype caused by an
insertional mutation.

Con:

1. More work (at least initially, but see 1 above).

It sounds as if you could use some better colony management, and also
a good way to check for transgene dosage in your offspring. There
are reasonably good hybridization and PCR-based protocols to do the
latter, though you should still progeny test suspected homozygotes.

Good luck,

Carlisle

>Hi all,
>
>My boss has this "thing" about making our transgenic lines homozygous -
>personally I don't think it's worth it, because most of the breeding is
>only done to maintain the lines.
>
>We basically have two cages of males for each line (approx 50 cages) and
>only breed them when he goes "oh crap, they're 10 months old, we'd better
>breed them or we'll lose the line!"
>
>As I say I can't see the point in breeding to obtain homozygous lines,
>which would take two or three generations to confirm, and generate heaps of
>cages, when three generations, as we do at the moment, would be bred over
>about 2 1/2 - 3 years.
>
>Any ideas for or against would be much appreciated.
>
>Thanks is advance
>
>Donna Roberts
>Transgenic Mouse Facility
>Hanson Centre for Cancer Research
>Institute of Medical and Veterinary Science
>Frome Road
>Adelaide 5000
>South Australia
>Ph: +61 8 8222 3473
>Fax: +61 8 8232 4092
>Email: donna.roberts@imvs.sa.gov.au
>
>"transgenic-list" web site (archives, FAQs, protocols, links) address:
>http://www.med.ic.ac.uk/db/dbbm/tglist.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From parker-thornburg.1 at osu.edu Mon Feb 14 15:45:02 2000
From: parker-thornburg.1 at osu.edu (Jan Parker-Thornburg)
Date: Wed Aug 20 11:55:26 2003
Subject: Fwd: Blastocysts
Message-ID: <v04210104b4ce43c0b7c7@[128.146.132.236]>

Saw this on another list. Can anyone from this group help her out?
E-mail Dale Cooper, please, not me. Thanks.

Jan

>
>The coordinator for our mouse manipulative genetics laboratory is seeking
>assistance from her colleagues in compmedland. For no apparent reason she
>has been getting poor harvests of blastocysts. There has been no change in
>mouse strain used, drugs or procedures. However, she is only getting about
>20% viable blastocysts at 2.5 days of harvest, and indicates that many of
>them are more developed than they should be. This is a dramatic change
>from normal and is seriously impeding work.
>
>If anyone out there has experienced anything similar or can give input on
>potential variables in blastocyst production such as humidity, phase of the
>moon, karmic cycle, etc. and would be willing to talk with her, please
>contact me and I will forward your name.
>
>Thanks
>
>
>Dale M. Cooper, DVM, MS, DACLAM
>Associate Program Director, Office of Regulatory Affairs
>Academic Health Center, University of Minnesota
>Box 501 Mayo, 420 Delaware St. SE
>Minneapolis, MN 55455
>(612) 625-0499; (612) 626-0178 (FAX); coope019@tc.umn.edu

Jan Parker-Thornburg, PhD
Manager, Transgenic Animal Facility
066 Rightmire Hall
1060 Carmack Rd.
The Ohio State University
Columbus, OH 43210
Phone: (614) 292-8715
Fax: (614) 292-5379
parker-thornburg.1@osu.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Donna.ROBERTS at imvs.sa.gov.au Tue Feb 15 08:46:24 2000
From: Donna.ROBERTS at imvs.sa.gov.au (Donna Roberts)
Date: Wed Aug 20 11:55:26 2003
Subject: Homozygous transgenic lines - thanks
Message-ID: <3.0.6.32.20000215084624.0079b400@mail.imvs.sa.gov.au>

Hi all (again!),

Thanks to everyone to replied to my message about homozygous lines. Good to
know I'm right (and "I told you so" is just Soooooo satisfying!!!)

For those of you who suggested freezing - we can't get it to work!! I've
tried two different protocols and fallen flat on my face both times - Bummer!

I am also the one who does the typing, so "he" has no really good reason
for wanting the lines as homozygous, except he doesn't know enough about
these things and doesn't listen to me either - hence the need for your help!

I shall show him the messages, and see where we go from there.

Thanks once again for your helpful advice

Regards

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From quitrent at hotmail.com Tue Feb 15 03:24:04 2000
From: quitrent at hotmail.com (Kerem OZGUNEN)
Date: Wed Aug 20 11:55:26 2003
Subject: mouse karyotype
Message-ID: <20000215112404.35744.qmail@hotmail.com>

Dear list,
Have any of you make karyotype from bone marrow ?
If so, can you please sent me the protocole you use .
Thanks

Kerem Tuncay OZGUNEN

Cukurova University
Faculty of Medicine
Department of Physiology
01330 Balcali / Adana
TURKEY

Tlf / Fax : +90 322 338 68 47

______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From besnard at biotec.jouy.inra.fr Tue Feb 15 14:01:29 2000
From: besnard at biotec.jouy.inra.fr (Nathalie Besnard)
Date: Wed Aug 20 11:55:26 2003
Subject: cesarean procedure
Message-ID: <3.0.1.32.20000215140129.006a65c4@biotec.jouy.inra.fr>

Hi everyone,

Does anybody know if it's possible to do a cesarean procedure without
killing the mouse ? We have a transgenic mouse who has had several
miscarriges and we are looking for a means to save both the mother and her
pups.

Thanks in advance for your advice.

Nathalie Besnard
INRA, laboratoire de G?n?tique Biochimique
Jouy en Josas 78352
France

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From i.rosewell at icrf.icnet.uk Tue Feb 15 17:15:07 2000
From: i.rosewell at icrf.icnet.uk (Ian Rosewell)
Date: Wed Aug 20 11:55:26 2003
Subject: amount
Message-ID: <l03130302b4cfa948165c@[143.65.49.35]>

Dear list

Could someone give me a ball park figure for the amount of DNA that could
be obtained from an ear disc of the size taken by an ear punch ( from a
mouse!).

ian

Ian Rosewell Tel : ++44 171 269 3778
Transgenic Services Fax : ++44 171 269 3953
ICRF Clare Hall Laboratories email: i.rosewell@icrf.icnet.uk
South Mimms, Potters Bar
Herts EN6 3LD
UK

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Tue Feb 15 14:17:17 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Wed Aug 20 11:55:26 2003
Subject: amount
References: <l03130302b4cfa948165c@[143.65.49.35]>
Message-ID: <38A9A63D.58121948@karmanos.org>

Ian Rosewell wrote:

> Could someone give me a ball park figure for the amount of DNA that could be
> obtained from an ear disc of the size taken by an ear punch ( from amouse!).
>
> http://www.med.ic.ac.uk/db/dbbm/tglist.htm

Never done it myself...I'd estimate you'd get ~15 mg of wet weight per punch,
containing on the order of 1-2 million nuclei (I assume that the punch is highly
cartilaginous, thus low cellularity). At 3 pg/nucleus that would be 3-6
micrograms of DNA, plenty for many PCR rxns but only ~1 Southern lane. [Tissue
cellularity also depends on how much blood you sample in a tissue, since blood
is highly cellular and 1 microliter of blood harbors ~5-10 10^3 nuclei, an
important contribution in highly vascular tissues such as liver and placenta.]

--
* * * * * * * * * * * * * * * * * *
Anton-Scott Goustin, Ph.D.
Assistant Professor
Program in Molecular Biology and Genetics
Karmanos Cancer Institute
110 East Warren (Prentis 311)
Detroit, Michigan USA 48201-1379
PHONE 313-833-0715 x2384
FAX 313-832-7294
* * * * * * * * * * * * * * * * * *

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From clandel at jax.org Tue Feb 15 14:38:22 2000
From: clandel at jax.org (Carlisle Landel)
Date: Wed Aug 20 11:55:26 2003
Subject: amount
In-Reply-To: <l03130302b4cfa948165c@[143.65.49.35]>
References: <l03130302b4cfa948165c@[143.65.49.35]>
Message-ID: <v0421010db4cf5a77491e@[199.94.155.139]>

>Dear list
>
>Could someone give me a ball park figure for the amount of DNA that could
>be obtained from an ear disc of the size taken by an ear punch ( from a
>mouse!).
>
>ian
>

Ian,

I *think* I recall that the ballpark figure for DNA content of
tissues is 1 mg DNA per gram (wet weight) of tissue. Your mileage
will vary, of course, based on how densely packed the cells (well,
nuclei) are in your tissue.

And this assumes that my recollection is correct! 8^)

Regards,

Carlisle

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From wayne.damcevski at anu.edu.au Wed Feb 16 11:05:04 2000
From: wayne.damcevski at anu.edu.au (Wayne Damcevski)
Date: Wed Aug 20 11:55:26 2003
Subject: Supliers of mice?
Message-ID: <3.0.6.32.20000216110504.0079b500@pophost.anu.edu.au>

Dear List,
Does anyone know a supplier for BXSB/MpJ and NZW/LacJ mice other than
Jackson Labs that can supply these mice to me before March 14 th 2000.

Cheers

Wayne
*-------------------------------------------------------*
* Wayne Damcevski *
* Breeding Colony Coordinator *
* Gene Targeting Mouse Facility *
* Centre For Molecular Stucture And Function *
* John Curtin School Of Medical Research *
* Canberra A.C.T. *
* Fax (02) 6249 0415 _ _ *
* Ph (02) 6279 8928 (_)-(_) *
* \"/ *
* =V= *

*=======================================================*

"If only I was a fly on the wall. How much gossip we can over hear" !!

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Donna.ROBERTS at imvs.sa.gov.au Wed Feb 16 10:43:48 2000
From: Donna.ROBERTS at imvs.sa.gov.au (Donna Roberts)
Date: Wed Aug 20 11:55:26 2003
Subject: Summary of replies re homozygous transgenics
Message-ID: <3.0.6.32.20000216104348.0079d840@mail.imvs.sa.gov.au>

Hi All,

The list owner has asked me to summarise the replies I got, so here goes:

FOR +/+: no more genotyping

AGAINST +/+: recombination of the transgene may inactivate the expression
seen in +/-
deleterious phenotypic effects not seen in +/-
more work and expense to obtain the +/+
more breeding (than we do currently) to maintain +/+
less than expected numbers of +/+ from 2 x +/- breeding
+/+ isn't necessary for experiments and may affect the results, which
have already been obtained using +/-

OTHER SUGGESTIONS: cryopreservation has the big thumbs up, for either eggs
or sperm.

I would also like to add my own reasons for not wanting to breed to +/+
- we are starting with only males so would have to breed for at least 3
generations (which would take 2 1/2 years at our current rate)
- we have no idea of the effect of the phenotype if we breed to +/+. They
all seem okay as +/-, but who knows!
- we have one founder who gave us progeny with 3 different copy numbers: he
was a two + one copy transgenic who gave one, two or three copy progeny.
Making this line +/+ would have been a nightmare!
- I rederived 17 of our lines in May/June after an MHV outbreak, and I'm
still waiting for my boss to do some experiments on them...hence the need
to only maintain, and the reason we only have males.
- I don't have the time to add to my current work load: I am the "idiot"
who does the microinjection (from superovulation to surgery), the screening
of the resulting litters by Southern, the breeding of the founders and the
maintenance of the resulting lines and the screening of all those babies by
southern. Currently we have 43 established lines and more on the way (as
soon as I have the time to do the screening!) I have 5 contructs ready to
be injected, and more in the pipeline. I am also expected to kill and
remove organs if/when my boss wants to do experiments - I work 5 1/2 days
as it is (bad tempered and grumpy - that's me!) and I keep trying to point
out to my boss that there is only one of me!

Anyway, I'm sure you must all have better things to do than listen to me
complain! Thanks once again for all your thoughts on the matter.

Regards,

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From szhao at bcm.tmc.edu Tue Feb 15 21:00:44 2000
From: szhao at bcm.tmc.edu (Shulei Zhao)
Date: Wed Aug 20 11:55:26 2003
Subject: needles for pronuclear injection
Message-ID: <Pine.GSO.4.10.10002152027150.8042-100000@watson.bcm.tmc.edu>

I have a few questions regarding microinjection and would like to know
the experiences of other researchers. Some of the questions may have been
posted before. Please excuse me if that's the case.

(1) the needle: break it or not?

In our lab, we normally break the tip of the injection needle by hitting
the holding needle. The Hogan's book (Manipulating mouse embryos) says
that one does not need to do that. We use a older needle puller made by
Sutter Instrument. The DNA never flows out if we don't break the needle
(we tried different parameters for needle pulling).

Do you break the needle or not? Are there machines that can pull needles
such that one does not have to break it before injection? Who are the
vendors?

(2) clogged injection needle

What are the primary causes for needle clogging? Impurities in DNA samples
, debris/dirts previously existed in capillaries or soemthing else? How to
prevent/eliminate them?

(3) How to prevent the formation of air bubbles in the injection needle?

Your comments and suggestions are appreciated.

Shulei Zhao
Baylor College of Medicine
Houston, TX 77030
USA

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Ying.Hu at flinders.edu.au Wed Feb 16 13:56:38 2000
From: Ying.Hu at flinders.edu.au (Yjng Hu)
Date: Wed Aug 20 11:55:26 2003
Subject: screening p53 knockouts
Message-ID: <3.0.2.32.20000216135638.008f2900@post.flinders.edu.au>

Dear list,

I am about to order a p53 knockout male from Jacksons Lab. I will be
breeding him to wildtype here and doing an experiment on the F1 generation,
both wildtype and heterozygote, and eventually p53 null (-/-). I'm
wondering if anyone has a proven screening protocol for this knockout.
Hopefully PCR, but southern would be OK.
Also, I'm new to transgenics, and would appreciate any information you
could give me on this line in regard to breeding etc.
With sincere thanks,
Ying Hu

Department of Gastroenterology
Flinders Medical Centre
Adelaide, Australia

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From epoch at ibv.csic.es Wed Feb 16 11:01:02 2000
From: epoch at ibv.csic.es (epoch@ibv.csic.es)
Date: Wed Aug 20 11:55:26 2003
Subject: p27 WT allele
Message-ID: <38AA836E.786F4BD1@ibv.csic.es>

Dear all:
We are genotyping a p27wild-type alele and it seems to be quite
difficult (I mean, sometimes it works, sometimes don't) .Could anybody
give us some advice about it?
Thanks
--
Enric Poch
Instituto de Biomedicina de Valencia (CSIC)
Unidad Biologia Vascular
Jaime Roig, 11
46010-Valencia
Spain
epoch@ibv.csic.es
Phone: 34-96-339 1751/1750
FAX: 34-96-369 0800

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tsaunder at umich.edu Wed Feb 16 07:49:08 2000
From: tsaunder at umich.edu (Thom Saunders)
Date: Wed Aug 20 11:55:26 2003
Subject: needles for pronuclear injection
In-Reply-To: <Pine.GSO.4.10.10002152027150.8042-100000@watson.bcm.tmc.edu>
Message-ID: <3748228.3159676148@host-83.subnet-31.med.umich.edu>

1. We break the needle on the holding pipet. We use a Sutter P-87
Flaming/Brown micropipet puller.

2. Needles clog when particulates are present in the DNA. We prefilter all
solutions used to resuspend DNA with 0.02 micron filters (Anotop). The pore
size is so small that it will trap DNA molecules greated than 1 Kb.

3. Bubble formation occurs when particulates trap air on the side of the
needle (see 2 above). Needle geometry also influences bubble trapping. A
slender needel with a long taper from tip to shoulder is more likely to
trap bubbles than a shorter one.

Sincerely,

Thom Saunders, Ph.D.
Transgenic Animal Model Core
University of Michigan Medical School
Ann Arbor, MI 48109-0674

email: tsaunder@umich.edu
URL: http://www.med.umich.edu/tamc/

--On Tue, Feb 15, 2000 9:00 PM -0600 Shulei Zhao <szhao@bcm.tmc.edu> wrote:

> I have a few questions regarding microinjection and would like to know
> the experiences of other researchers. Some of the questions may have been
> posted before. Please excuse me if that's the case.
>
>
> (1) the needle: break it or not?
>
> In our lab, we normally break the tip of the injection needle by hitting
> the holding needle. The Hogan's book (Manipulating mouse embryos) says
> that one does not need to do that. We use a older needle puller made by
> Sutter Instrument. The DNA never flows out if we don't break the needle
> (we tried different parameters for needle pulling).
>
> Do you break the needle or not? Are there machines that can pull needles
> such that one does not have to break it before injection? Who are the
> vendors?
>
> (2) clogged injection needle
>
> What are the primary causes for needle clogging? Impurities in DNA samples
> , debris/dirts previously existed in capillaries or soemthing else? How to
> prevent/eliminate them?
>
> (3) How to prevent the formation of air bubbles in the injection needle?
>
>
> Your comments and suggestions are appreciated.
>
>
>
> Shulei Zhao
> Baylor College of Medicine
> Houston, TX 77030
> USA

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From pozzi at crs.ifo.it Wed Feb 16 16:22:22 2000
From: pozzi at crs.ifo.it (Laura Pozzi)
Date: Wed Aug 20 11:55:26 2003
Subject: needles for pronuclear injection
Message-ID: <3.0.1.32.20000216162222.007647d0@crs.ifo.it>

- We break the needles against the holding pipette. The clipping is so
slight that one does not actually see any piece breaking off.

- Against clogging, we spin in minifuge the DNA injection solution and
collect carefully, leaving > 50 microl. at eppendorf bottom. The DNA is
purified from agarose with GeneClean with spin column, eluted with
injection buffer, and used directly with no precipitation.

- normally we do not have bubbles. It happend once in a severe fashion,
totally preventing DNA outflow - in desperation I heated the needles
(before pulling ) at about 120?C for 45 min in a glass test tube and the
problem disappeared. It must have been environment dampness. Now we
always "dry" a batch of neddles. to last for some microinjection sessions.
It may work also for your needles!
Hope it helps Laura
Dr.Laura Pozzi
Lab.Modelli Animali
Centro Ricerca Sperimentale
Istituto Regina Elena
Via delle Messi d'oro 156
00158 Roma, Italy
tel. -39 06 49852512
fax - 39 06 49852505
email pozzi@crs.ifo.it

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From haugenh at zgi.com Wed Feb 16 07:30:01 2000
From: haugenh at zgi.com (HHAU (Harald Haugen))
Date: Wed Aug 20 11:55:26 2003
Subject: needles for pronuclear injection
Message-ID: <C7903046C51AD3118AE30008C72486FF3AD1F6@ntuszg07.zgi.com>

> Shulei Zhao asks:
> (1) the needle: break it or not?
>
I say: Don't break the needle, etch it using hydrofluoric acid.
That way you can determine very specific how large a hole you want in the
needle. It will also be vey sharp and readily penetrate the pronucleus.

> (2) clogged injection needle
>
I say: Filter the DNA through a .2 filter prewashed with your
buffer. It should remove any gel or other particulate in your DNA.

> (3) How to prevent the formation of air bubbles in the injection needle?
>
I say: When you etch the needle, you can suck DNA into the needle,
this way no air bubbles should appear. Each needle can then be used for 50
- 100 embryos.

I also use the Sutter puller, and find it giving me the needles I
need.

Good luck, harald

> Harald S Haugen
> Associate Scientist
> Transgenics
> ZymoGenetics, Inc.
> 1201 Eastlake Avenue East
> Seattle, Washington 98102
> Tel: +1 206 515 4904
> Fax: + 1 206 515 4911
>
> haugenh@zgi.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From pas at aretha.jax.org Wed Feb 16 10:49:43 2000
From: pas at aretha.jax.org (Peter Schweitzer)
Date: Wed Aug 20 11:55:26 2003
Subject: screening p53 knockouts
Message-ID: <v03007804b4d07781f66a@[199.94.155.141]>

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From pas at aretha.jax.org Wed Feb 16 10:50:11 2000
From: pas at aretha.jax.org (Peter Schweitzer)
Date: Wed Aug 20 11:55:26 2003
Subject: p27 WT allele
Message-ID: <v03007805b4d077a9ffc1@[199.94.155.141]>

Hi Enric,

We are using a pair of PCR primers to amplify the Cckn1b (p27) wild type allele (these amplify no product from the homozygous knockout made by Matthew Fero) and they work fairly well for us:

oIMR947 5'-gAT ggA CgC CAg ACA AgC-3'
oIMR948 5'-CTC CTg CCA TTC gTA TCT gC-3'

wild type allele product = 190 bp.

If you want all the details of our protocols, email me privately and I will send you all the information.

Good luck,

Peter

Peter Schweitzer
The Jackson Laboratory
pas@jax.orgn

>Dear all:
>We are genotyping a p27wild-type alele and it seems to be quite
>difficult (I mean, sometimes it works, sometimes don't) .Could anybody
>give us some advice about it?
>Thanks
>--
>Enric Poch
>Instituto de Biomedicina de Valencia (CSIC)
>Unidad Biologia Vascular
>Jaime Roig, 11
>46010-Valencia
>Spain
>epoch@ibv.csic.es
>Phone: 34-96-339 1751/1750
>FAX: 34-96-369 0800
>
>
>
>"transgenic-list" web site (archives, FAQs, protocols, links) address:
>http://www.med.ic.ac.uk/db/dbbm/tglist.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From clandel at jax.org Wed Feb 16 10:55:26 2000
From: clandel at jax.org (Carlisle Landel)
Date: Wed Aug 20 11:55:26 2003
Subject: needles for pronuclear injection
In-Reply-To: <Pine.GSO.4.10.10002152027150.8042-100000@watson.bcm.tmc.edu>
References: <Pine.GSO.4.10.10002152027150.8042-100000@watson.bcm.tmc.edu>
Message-ID: <v04210104b4d077663cac@[199.94.155.139]>

At 9:00 PM -0600 2/15/00, Shulei Zhao wrote:
>I have a few questions regarding microinjection and would like to know
>the experiences of other researchers. Some of the questions may have been
>posted before. Please excuse me if that's the case.
>
>
>(1) the needle: break it or not?

I break the needle. I've used all sorts of needle pullers. You can
find settings that come close to the shape you want, and which even
have open ends.

>(2) clogged injection needle
>
>What are the primary causes for needle clogging? Impurities in DNA samples
>, debris/dirts previously existed in capillaries or soemthing else? How to
>prevent/eliminate them?

Junk in the DNA solution and also sometimes contamination picked up
from the egg. I clean my DNA solution by centrifugation for 10 min
at highest speed, and then (carefully!) pull off the supernatant,
leaving behind some of the solution at the bottom of the tube. When
my needle clogs, I just break it off some more. If you use a
continuous-flow system, as I do, you then just reduce the injection
pressure appropriately.

>(3) How to prevent the formation of air bubbles in the injection needle?

I just back-fill the needle (pulled from a capillary with a
filament). Sometimes a bubble forms somewhere in the needle, but,
unless it gets right up into the tip, it doesn't affect injection.

My 2 cents, your mileage may vary.

Carlisle

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From chenm0 at uthscsa.edu Wed Feb 16 10:45:13 2000
From: chenm0 at uthscsa.edu (Mingjiu Chen)
Date: Wed Aug 20 11:55:26 2003
Subject: needles for pronuclear injection
In-Reply-To: <Pine.GSO.4.10.10002152027150.8042-100000@watson.bcm.tmc.edu>
Message-ID: <v03007802b4d0846a0ca5@[129.111.240.165]>

Hi, Shulei;

1. After pulling the needle, I grinded it to make 45 degrees beveled tip.
You need to practice for a while to have yourself get the "right" needle.
2. I think you may got the needle clogged after several injections. Trying
to avoid suck the cytoplamic stuff in the injection needle, making your DNA
sample clean and not too thick. make your injection needle tip small enough
to inject pronuclei but big enough to let DNA sample go through easily; 3.
more DNA sample solution need to preload on your injection needle may help
you avoid air bubble formation.

good luck,

mingjiu

--------------
University of Texas
Health Science Center at San Antonio
15355 Lambda Drive
San Antonio, TX 78245

>I have a few questions regarding microinjection and would like to know
>the experiences of other researchers. Some of the questions may have been
>posted before. Please excuse me if that's the case.
>
>
>(1) the needle: break it or not?
>
>In our lab, we normally break the tip of the injection needle by hitting
>the holding needle. The Hogan's book (Manipulating mouse embryos) says
>that one does not need to do that. We use a older needle puller made by
>Sutter Instrument. The DNA never flows out if we don't break the needle
>(we tried different parameters for needle pulling).
>
>Do you break the needle or not? Are there machines that can pull needles
>such that one does not have to break it before injection? Who are the
>vendors?
>
>(2) clogged injection needle
>
>What are the primary causes for needle clogging? Impurities in DNA samples
>, debris/dirts previously existed in capillaries or soemthing else? How to
>prevent/eliminate them?
>
>(3) How to prevent the formation of air bubbles in the injection needle?
>
>
>Your comments and suggestions are appreciated.
>
>
>
>Shulei Zhao
>Baylor College of Medicine
>Houston, TX 77030
>USA
>
>
>
>"transgenic-list" web site (archives, FAQs, protocols, links) address:
>http://www.med.ic.ac.uk/db/dbbm/tglist.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From chenm0 at uthscsa.edu Wed Feb 16 10:59:08 2000
From: chenm0 at uthscsa.edu (Mingjiu Chen)
Date: Wed Aug 20 11:55:26 2003
Subject: p27 WT allele
In-Reply-To: <38AA836E.786F4BD1@ibv.csic.es>
Message-ID: <v03007803b4d0855c45b5@[129.111.240.165]>

>Dear all:
>We are genotyping a p27wild-type alele and it seems to be quite
>difficult (I mean, sometimes it works, sometimes don't) .Could anybody
>give us some advice about it?

This happened to me once, just carefully prepare the PCR reaction to make
sure it mixed very well. It's better to mix your reaction mixture by
peppitting:) before PCR .

hope it helps,

mingjiu

--------------
University of Texas
Health Science Center at San Antonio
15355 Lambda Drive
San Antonio, TX 78245

>Thanks
>--
>Enric Poch
>Instituto de Biomedicina de Valencia (CSIC)
>Unidad Biologia Vascular
>Jaime Roig, 11
>46010-Valencia
>Spain
>epoch@ibv.csic.es
>Phone: 34-96-339 1751/1750
>FAX: 34-96-369 0800
>
>
>
>"transgenic-list" web site (archives, FAQs, protocols, links) address:
>http://www.med.ic.ac.uk/db/dbbm/tglist.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From JHEWITT at niaid.nih.gov Wed Feb 16 12:19:07 2000
From: JHEWITT at niaid.nih.gov (Judith A. Hewitt)
Date: Wed Aug 20 11:55:26 2003
Subject: needles for pronuclear injection
Message-ID: <2181293698E2D011A74C006097B917172E2D72@ponyexpress.niaid.nih.gov>

A lot of good answers so far. The one thing not mentioned yet is
siliconizing the glass used to make needles, and I have always been curious
to know how many labs do this/don't do this. I think it helps keep needles
from getting clogged with cellular debris (though I've also always spun the
DNA first) as well as not get air bubbles that can't be easily dislodged.
You do have to backfill with a long thin pipette rather than by capillary
action. After filling the needles, set them in a foam block with slits,
angling them so the tip points downward and by the time you're ready with a
slide, there are no air bubbles left.

Also, I've done both beveling with silicon carbide (BioTechniques 14:412-4,
1993) & breaking against the holding pipette, and beveling is easier to
learn. Right now, we are using needles with two very different geometries
and trying to decide which is better, the open ones or the ones we need to
break/bevel. Both have advantages & disadvantages, and any puller should be
able to pull whatever you want to use.

Judy

Judith A. Hewitt, Ph.D.
Staff Scientist, TKMF/NIAID/NIH
Bldg. 550, Rm. 218
PO Box B, Ft. Detrick
Frederick, MD 21702
phone 301-846-5686
fax 301-846-6984
jhewitt@nih.gov
http://www.niaid.nih.gov/dir/services/mouse/info.htm
<http://www.niaid.nih.gov/dir/services/mouse/info.htm>

-----Original Message-----
From: Shulei Zhao [ mailto:szhao@bcm.tmc.edu <mailto:szhao@bcm.tmc.edu> ]
Sent: Tuesday, February 15, 2000 10:01 PM
To: transgenics
Subject: needles for pronuclear injection

I have a few questions regarding microinjection and would like to know
the experiences of other researchers. Some of the questions may have been
posted before. Please excuse me if that's the case.

(1) the needle: break it or not?

Do you break the needle or not? Are there machines that can pull needles
such that one does not have to break it before injection? Who are the
vendors?

(2) clogged injection needle

What are the primary causes for needle clogging? Impurities in DNA samples
, debris/dirts previously existed in capillaries or soemthing else? How to
prevent/eliminate them?

(3) How to prevent the formation of air bubbles in the injection needle?

Your comments and suggestions are appreciated.

Shulei Zhao
Baylor College of Medicine
Houston, TX 77030
USA

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm
<http://www.med.ic.ac.uk/db/dbbm/tglist.htm>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From rfranks at uic.edu Wed Feb 16 12:10:38 2000
From: rfranks at uic.edu (Roberta Franks)
Date: Wed Aug 20 11:55:26 2003
Subject: needles for pronuclear injection
Message-ID: <v04003a03b4d09812a629@[128.248.33.139]>

We use a Sutter P-87 puller; the tips are open when we pull them and the
DNA solution readily flows out - unless there is debris in the needle, in
which case we can see particles flowing down the needle into the tip and
clogging it. When this happens we can sometimes break the tip to form a
bigger opening by brushing it against the holding pipet (often the tip is
too big after doing this). To minimize debris we spin through a 0.2-0.4
micron filter (which, of course doesn't remove the smallest particulates).
Passing through an elutip column after gel-purification (we use GeneClean)
also helps.

About bubbles - our needles are pulled from capillaries containing a fine
filament, which are designed for rapid-fill (they fill easily due to
enhanced capillary action). You can purchase these from Sutter (Novato, CA
415-883-0128) or from Frederick Haer (Bowdoinham, ME 207-666-8190). Since
the needles are open when we pull them they can be filled from the back end
(via capillary action), either by placing the back end down into the DNA
solution or by using a long filling-pipet tip (Eppendorf), which fits way
down into most of the needle (close to the tip).

Roberta R. Franks, Ph.D.
Director, UIC Transgenic Mouse Production Service
University of Illinois at Chicago M/C 669
Building 910
900 S. Ashland Avenue
Chicago, IL 60607-7170
ph 312-996-4971
fx 312-413-3128

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From xinyu at ucdavis.edu Wed Feb 16 10:23:41 2000
From: xinyu at ucdavis.edu (xin yu)
Date: Wed Aug 20 11:55:26 2003
Subject: female ES cell line
Message-ID: <3.0.3.32.20000216102341.006ba968@yellow.ucdavis.edu>

Dear list,

I'm looking for a female ES cell line that goes germline. I've checked the
archive, and the same question was asked by another person two years ago.
Are there any new or commercial sources currently available?

Thanks in advance!

Xin Yu
Targeted Genomics Laboratory
University of California, Davis
(530)752-3004

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From cali at liai.org Wed Feb 16 15:05:25 2000
From: cali at liai.org (Chris lena)
Date: Wed Aug 20 11:55:26 2003
Subject: ES cell strains
Message-ID: <v04210102b4d0cf511714@[172.20.1.64]>

Dear list,
Does anybody know the conditions to differentiate ES cells in
different tissues?
Thanks,
chris

Chris Lena
Laboratory Technician
La Jolla Institute For Allergy and Immunology
phone:(858)678-4536
fax:(858)678-4595
cali@liai.org
http://www.liai.org

_ _
(_)-(_)
\"/
=V=

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From elena.notarianni at ncl.ac.uk Thu Feb 17 10:36:38 2000
From: elena.notarianni at ncl.ac.uk (Elena Notarianni)
Date: Wed Aug 20 11:55:26 2003
Subject: female ES cell line
Message-ID: <200002171035.KAA28809@cheviot1.ncl.ac.uk>

Try Dr. Anne K Voss (avoss@gwdg.de) at the Max-Planck Institute in
Gottingen.
Regards, Elena

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From GWENDOLYN.T.WONG at spcorp.com Thu Feb 17 08:41:00 2000
From: GWENDOLYN.T.WONG at spcorp.com (WONG, GWENDOLYN)
Date: Wed Aug 20 11:55:26 2003
Subject: Stem cell research
Message-ID: <200002171346.IAA19929@sp2.spcorp.com>

Dear colleagues,

Many of you may have received and responded to this NIH bulletin already.
If
so, my apologies for the redundancy. The NIH is requesting comments on this
issue, due to overwhelmingly negative responses from other groups with the
opposite point of view. I urge you all to consider sending an email
response.

Gwen Wong
Dept. CNS/CV Discovery Research
Schering-Plough Research Institute
Kenilworth NJ 07033

****************************************************************************
****

BULLETIN:

The National Institutes of Health has extended the deadline for public
comments on draft guidelines for federal funding of stem cell research until
February 22nd. We encourage you to send in your positive comments to the
NIH if
you haven't already done so. Tens of thousands of comments already tallied
are
heavily against approval of the draft guidelines. It is critically important
that as many scientists respond as possible.

Please urge colleagues and friends to voice support, too. Responding is
easy and fast. "I support the NIH guidelines for federal funding of human
pluripotent stem cell research" will suffice.

For a look at the guidelines, see:

http://www.nih.gov/news/stemcell/draftguidelines.htm

Responses can be sent to NIH by email:

stemcell@mail.nih.gov
by fax:
301/402-0280
or, by regular mail:

Stem Cell Guidelines, NIH Office of
Science Policy, 1 Center Drive, Building 1, Room 218
Bethesda, MD 20892.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From lonna at brc.ubc.ca Thu Feb 17 10:16:36 2000
From: lonna at brc.ubc.ca (Lonna)
Date: Wed Aug 20 11:55:27 2003
Subject: No subject
Message-ID: <v03110700b4d1e9e9d360@[137.82.2.89]>

Our lab is in the process of setting up a transgenic unit. We have
purchased Eppendorf micromanipulators and we already have an old phase
contrast Zeiss IM microscope. We would like to save costs and equip it with
Hoffman modulation optics to do DNA microinjections in mice. We are aware
that DIC is better, but would like to explore the Hoffman option. Does
anyone have any experience with Hoffman optics or does anyone have any
comments regarding DIC versus Hoffman? Thanks in advance.
-Lonna Miller

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From DonovanD at grc.nia.nih.gov Fri Feb 18 07:58:12 2000
From: DonovanD at grc.nia.nih.gov (Donovan, Dave)
Date: Wed Aug 20 11:55:27 2003
Subject: cryopreservation facilites
Message-ID: <C5BE6D142223D211909E0000F8CD228A014AC5C4@c3.grc.nia.nih.gov>

Dear List,

I have been asked to establish a murine cryopreservation facility for the
National Institute on Aging, NIH. We currently have more than 80 lines of
transgenic and knockout models that we would like to preserve (some created here
and others imported). I anticipate this will require much more than one year
with our current staffing/resources. In order to help me to prioritize the
order of freezing for these lines, I am attempting to compile a list of web
sites (and/or individuals) where records are maintained about lines that have
already been frozen. I want to ask the NIA investigator to research these
sites, in order to find out 1) if any of the lines slated for freezing here have
been previously frozen elsewhere, and 2) if so, whether or not the other
facilities might share their stocks with us if necessary, thus reducing the
immediate need to freeze some lines.

Can you please send me any information you have regarding web sites and/or
individuals who would be willing to share this information about their
cryopreserved stocks? I will summarize the results for the list.

I am currently aware of these sites :
NIH Animal Genetic Resource
http://dirs.info.nih.gov/intramur/vrp/open/nihagr.htm
Jackson Lab Induced Mutant Resource.
http://www.jax.org/resources/documents/imr
Mouse Knockout & Mutation Database http://www.biomednet.com/db/mkmd

Thank you in advance.

Dave

David M. Donovan, Ph.D.
Chief, Transgenic and Knockout Facility Section
Gerontology Research Center, IRP, NIA, NIH
5600 Nathan Shock Drive
Baltimore, MD 21224-6825
410-558-8111 voice
410-558-8236 fax

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From l.mcdonald at victorchang.unsw.edu.au Tue Feb 22 18:17:24 2000
From: l.mcdonald at victorchang.unsw.edu.au (Lachlan McDonald)
Date: Wed Aug 20 11:55:27 2003
Subject: help with ES cell culture
Message-ID: <l03130301b4d7e6cd860e@[129.94.42.71]>

Howdy,

Some questions re ES cell culture, thanks if you can help out.

1. Does anyone you regulary use alkaline phosphatase status as a marker of
the undifferentiated state of ES cells? Does anyone have experience of
using alk phos as a marker, ie how relaible is it felt to be?

2. Could anyone direct me to a detailed reference for serum screening for
ES cells? Exactly what are the criteria for a good serum batch ?

Thanks !

Lachlan

----------------------------------------------------------------------
Lachlan McDonald
Developmental Biology Group
Victor Chang Cardiac Research Institute
St. Vincents Hospital
384 Victoria St
Darlinghurst, Sydney
NSW 2010
Australia

Ph: (02) 9295 8522 Fax : (02) 9295 8501
http://www.victorchang.com.au
----------------------------------------------------------------------

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From iperez at ibv.csic.es Tue Feb 22 18:43:28 2000
From: iperez at ibv.csic.es (Ignacio Perez Roger)
Date: Wed Aug 20 11:55:27 2003
Subject: lacZ rabbit/rat
Message-ID: <38B2CAC0.8657A913@ibv.csic.es>

Dear list,
Can anybody tell me where to find any information about lacZ transgenic
rabbits or rats?
Cheers!
Nacho.

--
Ignacio Perez-Roger
Instituto de Biomedicina de Valencia (CSIC)
Jaime Roig, 11
46010-Valencia
Spain
iperez@ibv.csic.es
Phone: 34-96-339 1751/1750
FAX: 34-96-369 0800

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From sfk at bilbo.bio.purdue.edu Tue Feb 22 14:41:45 2000
From: sfk at bilbo.bio.purdue.edu (Steve Konieczny)
Date: Wed Aug 20 11:55:27 2003
Subject: Director, Transgenic Facility Position
Message-ID: <4.1.20000222143907.00a13890@bilbo.bio.purdue.edu>

Dear Colleagues,

The Purdue University NCI-designated Cancer Center has an immediate opening for
an individual to assume operational responsibility for the newly expanded
Purdue Cancer Center Transgenic Mouse Core Facility (TMCF). The TMCF enjoys
broad financial support from both the NCI as well as Purdue University with
guaranteed recurring funds to support this endeavor. This is an ideal position
for an individual who would like to take on the challenges of overseeing this
newly expanded Facility. The TMCF offers both transgenic and knock-out
services to the Purdue community as well as to other investigators in the
state. We are looking for someone who has knowledge and direct injection
experience in generating both model systems as well someone who enjoys being
able to completely oversee the many duties that are associated with running
this facility.

Please feel free to circulate this notice to any individual or laboratory that
you might think would be interested in this position. If there are specific
questions feel free to contact me directly at the address below. Thank you for
taking the time to read this notice and for helping us to find a qualified
individual.

Sincerely,

Stephen F. Konieczny, Professor
Department of Biological Sciences
Purdue University
West Lafayette, IN 47907-1392

Tel: 765-494-7976
Fax: 765-496-2536
email: sfk@bilbo.bio.purdue.edu

____________________________________________________________________________
____________

Research Scientist, Transgenic Mouse Core Facility
Purdue University Cancer Center

Purdue University is an Equal Opportunity/Affirmative Action Employer.

____________________________________________________________________________
____________
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From l-doglio at nwu.edu Wed Feb 23 09:44:28 2000
From: l-doglio at nwu.edu (lynn t. doglio)
Date: Wed Aug 20 11:55:27 2003
Subject: Position available
Message-ID: <v04011702b4d9aae3255d@[199.125.26.19]>

The Northwestern University/CMIER Transgenic Facility has an open research
technician position. Our core service facility provides pronuclear and
blastocyst injections for the research community at Northwestern
University. Experience is preferred in generating transgenic animals by
pronuclear injection, microsurgery, embryo manipulation and animal
handling. Research opportunities are available for highly motivated
individuals.

Interested individuals should send inquires to:

Lynn Doglio, PhD
Associate Director
NU/CMIER Transgenic Facility
Northwestern University Medical School
Department of Pediatrics
2300 Children's Plaza, MC204
Chicago, IL 60614

e-mail: l-doglio@nwu.edu
Phone: 773.880.8167
--
lynn doglio, Ph.D.
Associate Director
NU/CMIER Transgenic Facility
Northwestern University Medical School
Department of Pediatrics
2300 Children's Plaza, MC204
Chicago, IL 60614

Phone (773)880-8167
FAX (773)880-8266
e-mail l-doglio@nwu.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From JHEWITT at niaid.nih.gov Thu Feb 24 08:59:38 2000
From: JHEWITT at niaid.nih.gov (Judith A. Hewitt)
Date: Wed Aug 20 11:55:27 2003
Subject: light/dark cycles
Message-ID: <2181293698E2D011A74C006097B917172E2DA9@ponyexpress.niaid.nih.gov>

We have a small animal facility that is dedicated to the production &
breeding of transgenic & knockout mice, currently on a 12/12 light dark
cycle. I would like to do a simple survey, as we're considering changing to
14/10. Please email me directly with your light cycle and I'll summarize
for the list. Anyone with experience changing light cycles, please give me
more details!
Thanks,
Judy

Judith A. Hewitt, Ph.D.
Staff Scientist, TMF/NIAID/NIH
Bldg. 550, Rm. 218
PO Box B, Ft. Detrick
Frederick, MD 21702
phone 301-846-5686
fax 301-846-6984
jhewitt@nih.gov
http://www.niaid.nih.gov/dir/services/mouse/info.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From DANIEL.ROHRER at ROCHE.COM Thu Feb 24 17:34:54 2000
From: DANIEL.ROHRER at ROCHE.COM (Rohrer, Daniel {Geno~Palo Alto})
Date: Wed Aug 20 11:55:27 2003
Subject: Job opening at Roche Bioscience
Message-ID: <418F66121D9DD311A54B0000F80197881C8154@rplmsem1.pal.roche.com>

Dear all,
We currently have an opening for a Research Associate II/III at Roche
Bioscience in Palo Alto, CA.

JOB DESCRIPTION
This position will play an important role in the creation, maintenance, and
analysis of transgenic rodent models. These models are used for early-stage
validation of drug targets. The successful candidate will be the principal
microinjectionist for the business unit, carrying out all pronuclear and ES
cell microinjections. The candidate will also be responsible for genotyping
and maintenance of selected mouse colonies, and will carry out small animal
surgeries important for phenotype analysis. The candidate will be part of a
small, highly interactive and focused gene targeting/ transgenics group.

REQUIREMENTS
BS/MS or equivalent in Molecular or Cellular Biology, Physiology, or related
discipline, with 1-3 years job experience in the laboratory. Experience and
competence in all aspects of the transgenic/ES cell microinjection process
including both pronuclear and blastocyst microinjections, as well as all of
the relevant small animal surgical techniques required for this technology.
Animal husbandry and basic animal colony management skills are essential, as
well as some surgical expertise outside of the transgenic/ knockout needs.
Proficiency with basic molecular biology techniques such as DNA isolation,
PCR, and Southern blots will be needed. Must be comfortable with database
entry and maintaining files related to colony management (Excel, Word, etc).

If interested, please apply directly to me (address and email below).

Daniel Rohrer, Ph.D.
Roche Bioscience
Neurobiology, R2-101
3401 Hillview Ave.
Palo Alto, CA 94304
(650) 354-2684
(650) 855-1238 FAX
daniel.rohrer@roche.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Fred.Sablitzky at nottingham.ac.uk Fri Feb 25 14:04:20 2000
From: Fred.Sablitzky at nottingham.ac.uk (Fred Sablitzky)
Date: Wed Aug 20 11:55:27 2003
Subject: position available
Message-ID: <120B0AC7B68@pdn1.gene.nottingham.ac.uk>

Post-doctoral position available

Applications are invited for a post-doctoral research position funded by the Wellcome Trust
for up to three years. In collaboration with Dr Anderson and Prof. Lieberman, University
College London, we want to determine the role and importance in axonal regeneration of
growth-related genes such as GAP-43. We will use a novel gene targeting approach based
on the Cre/loxP recombination system. The candidate will be responsible to flank the target
genes with loxP sites and to establish mutant mice (this work will be done in Nottingham).
Subsequent delivery of Cre and analysis of axonal regeneration will be performed in
London.
The highly motivated candidate should have experience in molecular biology, embryonic
stem cells and in neurogenesis.

The position is available from the 1 April 2000. Salary will be on the RAIA scale.
Please send your CV and names and address of two references to

fred.sablitzky@nottingham.ac.uk

---- NEW address ----

Prof. Fred Sablitzky
The University of Nottingham
Institute of Genetics
Queen's Medical Centre
Nottingham
NG7 2UH

phone: 0115 849 3242
fax: 0115 849 3243
email: fred.sablitzky@nottingham.ac.uk
http://www.nottingham.ac.uk/genetics/Fred%20Sablitzky.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jkoster at cellbio.wustl.edu Fri Feb 25 11:05:21 2000
From: jkoster at cellbio.wustl.edu (Joseph Koster)
Date: Wed Aug 20 11:55:27 2003
Subject: Promiscuous promoters and mosaicism
Message-ID: <2.2.32.20000225170521.0072e3cc@cellbio.wustl.edu>

Hello,
A couple of questions from a novice: I'm interested in expressing a
transgene in the whole mouse and was wondering what promiscuous promoters
are available and their specificity(or lack thereof)? References would be
greatly appreciated. Also, what is the incidence of mosaicism in founder
mice? I gotten several,varied answers on this one.Thanks for your help!
Joseph Koster
-----------------------------------------------------
Joseph C. Koster
Dept. of Cell Biology and Physiology, Box 8228
Washington University School of Medicine
660 South Euclid Avenue
St. Louis, MO 63110

(314) 362-6629
(314) 362-7463 FAX
jkoster@cellbio.wustl.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From haugenh at zgi.com Fri Feb 25 09:37:29 2000
From: haugenh at zgi.com (HHAU (Harald Haugen))
Date: Wed Aug 20 11:55:27 2003
Subject: Promiscuous promoters and mosaicism
Message-ID: <C7903046C51AD3118AE30008C72486FFAF59BD@ntuszg07.zgi.com>

Joseph:

To give one possible answer to the second question:

If one assumes that insertion of the transgene is possible associated with
many of the early cell divisions, and one assume that the possibility of
gaining an insert is independent of whether an insert already has happened,
or will happen; then I believe that at each cell division a proportion of
the eggs I inject, in which there is still enough free transgene, may
incorporate an insert. This, in my experience, leads to that somewhat more
than 20% of my injected embryos are transgenic, 20% of the transgenics have
more than one insert, and 20% of the transgenics are mosaic.

Enjoy, harald

Harald S Haugen
Associate Scientist
Transgenics
ZymoGenetics, Inc.
1201 Eastlake Avenue East
Seattle, Washington 98102
Tel: +1 206 515 4904
Fax: + 1 206 515 4911

haugenh@zgi.com

> ----------
> From: Joseph Koster
> Reply To: Joseph Koster
> Sent: Friday, February 25, 2000 9:05 AM
> To: transgenic-list@ic.ac.uk
> Subject: Promiscuous promoters and mosaicism
>
> Hello,
> A couple of questions from a novice: I'm interested in expressing a
> transgene in the whole mouse and was wondering what promiscuous promoters
> are available and their specificity(or lack thereof)? References would be
> greatly appreciated. Also, what is the incidence of mosaicism in founder
> mice? I gotten several,varied answers on this one.Thanks for your help!
> Joseph Koster
> -----------------------------------------------------
> Joseph C. Koster
> Dept. of Cell Biology and Physiology, Box 8228
> Washington University School of Medicine
> 660 South Euclid Avenue
> St. Louis, MO 63110
>
> (314) 362-6629
> (314) 362-7463 FAX
> jkoster@cellbio.wustl.edu
>
>
> "transgenic-list" web site (archives, FAQs, protocols, links) address:
> http://www.med.ic.ac.uk/db/dbbm/tglist.htm
>
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From aannala at mednet.ucla.edu Fri Feb 25 09:48:11 2000
From: aannala at mednet.ucla.edu (Alexander J. Annala)
Date: Wed Aug 20 11:55:27 2003
Subject: Matrix Attachment regions (Re: Promiscuous promoters)
Message-ID: <3.0.32.20000225094807.007d27f0@medmail4.mednet.ucla.edu>

Dear Jonathan,

The problem is not just one of which promoter to use --
rather it also includes the position where your gene is
randomly integrated vs upstream and downstream enchancers,
repressors, and potential cryptic promoters. You should
consider bracketing your construct with some small MAR
(matrix attachment region) constructs to insulate new gene
from up/down regulation by adjacent chromosomal regions --
incl methylation and heterochromatin condensation effects.

Bracketing your gene with MAR constructs tends to provide
independence from position effects as well as copy number
dependent amplification of reporter gene expression.

Alexander J. Annala, Ph.D.
UCLA School of Medicine

At 11:05 AM 2/25/00 -0600, you wrote:
>Hello,
>A couple of questions from a novice: I'm interested in expressing a
>transgene in the whole mouse and was wondering what promiscuous promoters
>are available and their specificity(or lack thereof)? References would be
>greatly appreciated. Also, what is the incidence of mosaicism in founder
>mice? I gotten several,varied answers on this one.Thanks for your help!
>Joseph Koster
>-----------------------------------------------------
>Joseph C. Koster
>Dept. of Cell Biology and Physiology, Box 8228
>Washington University School of Medicine
>660 South Euclid Avenue
>St. Louis, MO 63110
>
>(314) 362-6629
>(314) 362-7463 FAX
>jkoster@cellbio.wustl.edu
>
>
>"transgenic-list" web site (archives, FAQs, protocols, links) address:
>http://www.med.ic.ac.uk/db/dbbm/tglist.htm
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From maria.alexiou at csc.mrc.ac.uk Fri Feb 25 18:35:32 2000
From: maria.alexiou at csc.mrc.ac.uk (Maria Alexiou)
Date: Wed Aug 20 11:55:27 2003
Subject: nod mice
Message-ID: <v01540b00b4dc6d6cbab1@[146.179.68.52]>

many thanks to all those who replied to our questions on nod mice-egg
performance/handling
Maria

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tsaunder at umich.edu Fri Feb 25 15:12:02 2000
From: tsaunder at umich.edu (Thom Saunders)
Date: Wed Aug 20 11:55:27 2003
Subject: Promiscuous promoters and mosaicism
In-Reply-To: <2.2.32.20000225170521.0072e3cc@cellbio.wustl.edu>
Message-ID: <36102595.3160480322@host-83.subnet-31.med.umich.edu>

Here are some papers for you to look up.

The Rosa26 promoter (Kisseberth, et al., 1999) is widely expressed in
transgenic mice.

The CMV promoter (Schmidt, et al., 1990, Furth et al., 1991) has been used
in many studies to obtain widespread gene expression.

Whitelaw et al., 1993 concluded that 62% of transgenic founders are mosaic.
Wilke at al., 1986 looked at 262 founder pedigrees and concluded 30% of
founders are mosaic.

Furth PA Hennighausen L Baker C Beatty B Woychick R
The variability in activity of the universally expressed human
cytomegalovirus immediate early gene 1 enhancer/promoter in transgenic
mice.
In: Nucleic Acids Res (1991 Nov 25) 19(22):6205-8

Kisseberth WC, Brettingen NT, Lohse JK, Sandgren EP. Ubiquitous expression
of marker transgenes in mice and rats. Dev Biol 1999 Oct 1;214(1):128-38

Schmidt EV Christoph G Zeller R Leder P
The cytomegalovirus enhancer: a pan-active control element in transgenic
mice.
In: Mol Cell Biol (1990 Aug) 10(8):4406-11

Whitelaw CB, Springbett AJ, Webster J, Clark J The majority of G0
transgenic mice are derived from mosaic embryos. Transgenic Res 1993
Jan;2(1):29-32

Wilkie TM, Brinster RL, Palmiter RD Germline and somatic mosaicism in
transgenic mice.
Dev Biol 1986 Nov;118(1):9-18 .

Sincerely yours,

Thom

Thom Saunders, Ph.D.
Transgenic Animal Model Core
Biomedical Research Core Facilities
University of Michigan Medical School
2560 MSRB II Box 0674
1150 W. Med. Center Dr.
Ann Arbor, MI 48109-0674

email: tsaunder@umich.edu
TEL: (734) 647-2910
FAX: (734) 936-2622
URL: http://www.med.umich.edu/tamc/

--On Fri, Feb 25, 2000 11:05 AM -0600 Joseph Koster
<jkoster@cellbio.wustl.edu> wrote:

> Hello,
> A couple of questions from a novice: I'm interested in expressing a
> transgene in the whole mouse and was wondering what promiscuous promoters
> are available and their specificity(or lack thereof)? References would be
> greatly appreciated. Also, what is the incidence of mosaicism in founder
> mice? I gotten several,varied answers on this one.Thanks for your help!
> Joseph Koster
> -----------------------------------------------------
> Joseph C. Koster
> Dept. of Cell Biology and Physiology, Box 8228
> Washington University School of Medicine
> 660 South Euclid Avenue
> St. Louis, MO 63110
>
> (314) 362-6629
> (314) 362-7463 FAX
> jkoster@cellbio.wustl.edu
>
>
> "transgenic-list" web site (archives, FAQs, protocols, links) address:
> http://www.med.ic.ac.uk/db/dbbm/tglist.htm

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From amanda.mcguigan at kcl.ac.uk Fri Feb 25 20:29:34 2000
From: amanda.mcguigan at kcl.ac.uk (Dr Amanda McGuigan)
Date: Wed Aug 20 11:55:27 2003
Subject: Water purification
Message-ID: <SIMEON.10002252034.E@muahost.kcl.ac.uk>

Dear List,
Can people give me any information on the extent to which animal
drinking water should be purified (for example : filters / UV /
chlorine addition) in a large clean animal facility.
Thanks,
Amanda

----------------------
Dr Amanda McGuigan
King's College
London SE1 9RT

amanda.mcguigan@kcl.ac.uk

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From boris at mdb.nhlbi.nih.gov Fri Feb 25 17:36:10 2000
From: boris at mdb.nhlbi.nih.gov (Vaisman, Boris (NHLBI))
Date: Wed Aug 20 11:55:27 2003
Subject: Promiscuous promoters and mosaicism
Message-ID: <29057D45C11BD211B68600805FEAA1EE125ABF@nihexchange3.nih.gov>

Dear Joseph:

Here are two more good papers about suitable house-keeping promoters:
1) Araki e.a., J. Biochem., 122, 977-982, 1997;
2) Bawden e.a., Transgenic Res., 4, 87-104, 1995.

Regarding your question about mosaicism in founder mice. I don't have ready
statistics of my own experience, but I do had a lot of cases where founder had
transgene in at least 2-3 different places with different number of transgene in
each (for example, 15 and 120). This gave us the great possibility to generate
transgenic lines with different number of transgene (15, 30, 120 and 240) and as
a result to study the dependence of the effects of the gene from its dose (which
in this case was linear). I practically never had really mosaic founder.

Dr. Boris L. Vaisman, Ph.D.
Staff Scientist
Molecular Disease Branch,
National Heart, Lung and Blood Institute,
National Institutes of Health
Building 10, Room 7N116,
10 Center Drive MSC 1666
Bethesda, MD 20892-1666,
Phone 301-496-3210
FAX 301-402-0190
E-mail Boris@MDB.NHLBI.NIH.GOV

-----Original Message-----
From: Joseph Koster [mailto:jkoster@cellbio.wustl.edu]
Sent: Friday, February 25, 2000 12:05 PM
To: transgenic-list@ic.ac.uk
Subject: Promiscuous promoters and mosaicism

(314) 362-6629
(314) 362-7463 FAX
jkoster@cellbio.wustl.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From boris at mdb.nhlbi.nih.gov Fri Feb 25 18:17:42 2000
From: boris at mdb.nhlbi.nih.gov (Vaisman, Boris (NHLBI))
Date: Wed Aug 20 11:55:27 2003
Subject: Homozygous transgenic lines
Message-ID: <29057D45C11BD211B68600805FEAA1EE125AC0@nihexchange3.nih.gov>

Dear List:

Recently there was small discussion on this list about usefulness of
using homozygous lines of transgenic mice, initiated by Donna Roberts.
Unfortunately, I was not able to participate at that moment, however, I am also
not able to agree with the conclusions.
Our lab's experience give me the possibility to say that the using of
homozygous lines is really very fruitful. For the other hand, the high
probability that transgenic founder will posses transgene in several different
places with different numbers of transgene per place, demand that offspring were
analyzed not only on the presence of transgene, but also on the number of copies
of this gene. In other case the study performed on a mixture of transgenic
animals with the different numbers of transgene localized in different places
will give some time too large variability of results. We are created and keeping
(some time more than 6 years) several different homozygous lines of mice and
rabbits, and we constantly thought that by working this way we not only used
correct genetic approach, but also saved a lot of time and money.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From sstice at arches.uga.edu Sun Feb 27 15:42:36 2000
From: sstice at arches.uga.edu (Steve Stice)
Date: Wed Aug 20 11:55:27 2003
Subject: gancyclovir and the placental barrier
Message-ID: <LOBBLKBOOFDGFLPAEBFBMEJMCFAA.sstice@arches.uga.edu>

We wish to do selection studies on transgenic embryos developing in utero.
My question is whether gancyclovir can cross the placental barrier?
References?

Thanks

Steve

Steven Stice, PhD
Associate Professor and Senior Research Scientist
ADS Complex, University of Georgia
Athens, GA 30602
tel 706-583-0071
fax 706-542-7925
sstice@arches.uga.edu

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From reule at atugen.com Mon Feb 28 12:26:50 2000
From: reule at atugen.com (Matthias Reule)
Date: Wed Aug 20 11:55:27 2003
Subject: reporter construct
Message-ID: <002301bf81de$b55bac30$2074508d@fgh.mdcberlin.de>

Dear list,
we try to optimize our EPO conditions. For this reason we want to electroporate a reporter construct ( lacZ or GFP ) under the control of the PGK-promoter. Does anyone know if this construct is commercially available or can someone provide us this construct?

Matthias Reule Ph.D.
Atugen biotechnology AG
Robert-Roessle Str. 10
D-13125 Berlin
Fon +49 (0)177 8489273
Fax +49 (0) 30 94064517
e-mail: reule@atugen.com
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From JHEWITT at niaid.nih.gov Mon Feb 28 09:13:38 2000
From: JHEWITT at niaid.nih.gov (Judith A. Hewitt)
Date: Wed Aug 20 11:55:27 2003
Subject: mosaicism/transmission in transgenics
Message-ID: <2181293698E2D011A74C006097B917172E2DD2@ponyexpress.niaid.nih.gov>

Hi,
I'm cross posting this to both lists, since one is discussing mechanism of
mosaics and the other frequency. I think that one needs to be somewhat
cautious about what is called a mosaic before drawing any conclusions about
their frequency. Now that I'm running a facility where the PI's labs do the
genotyping and PCR is frequently the method used to identify founders, we
find a lot (IMHO) of founders that don't transmit the transgene.
Previously, doing the genotyping myself by Southerns, I would call a
non-transmitter a mosaic, where tail tissue had the transgene & gametes
didn't (without looking at any other tissues). Now, my experience is that
the PI/lab might label these mice mosaic and I might call them false
positives, partly because I feel the frequency is too high (that's a feeling
folks and I'm not going to attach a number to it, yet). On the other hand,
we injected a construct and one of the founders was split into 3 sublines
based on different Southern patterns, and whether I believe that it's worth
it to maintain 3 lines is still an open question. We do a pretty good
amount of work here and I have thought that I would try to figure out mosaic
frequency from my database, but I'm afraid the number wouldn't be very
accurate if it included false positives. The ultimate answer would come
from actually showing that tissues from a specific mouse varied by presence
of the transgene and/or hybridization pattern, and I don't think that
experiment has been done very often, because as Lluis said, we just hope for
better next time. We tend not to vary DNA concentration or time of
injection very much, though we do change strains.

Now, if you want to know the incidence of non-transmission, whether it be
due to mosaicism or false positives or whatever, then I could give you a
number...
Judy

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From gfingerle at picower.edu Mon Feb 28 18:09:48 2000
From: gfingerle at picower.edu (gfingerle@picower.edu)
Date: Wed Aug 20 11:55:27 2003
Subject: deletor Cre mouse on C57Bl/6 background
Message-ID: <E12PZBq-0004bj-00@science.picower.edu>

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From paul.verma at adelaide.edu.au Tue Feb 29 15:41:51 2000
From: paul.verma at adelaide.edu.au (Paul Verma)
Date: Wed Aug 20 11:55:27 2003
Subject: Positions in Cell Biology
Message-ID: <7735ED7947F.AAA5E65@bloodwood.adelaide.edu.au>

STEM CELL AND CELL CYCLE BIOLOGISTS / PROTEIN CHEMIST / NUCLEAR TRANSFER
The Biochemistry Department, University of Adelaide announces six exciting
post-doctoral positions, in areas of research related to stem cell biology.
These will be supported by the department's interactions with three
organisations: the Pest Animal Cooperative Research Centre, the Special
Research Centre for Molecular Developmental Biology, and a major research
initiative directed towards the exploitation of stem cell biology, for
novel human cell-based therapies. This program, funded at $6 million over 3
years by BresaGen Ltd, is directed by Professor Peter Rathjen; administered
by Luminis Ltd, the University's technology transfer division and Dr. Paul
Tolstoshev of BresaGen will manage the commercial aspects of the program.
There are four research programs covering 1) Pluripotent stem cell
differentiation and dedifferentiation, 2) Cell reprogramming through
nuclear transfer, 3) Human stem cell isolation and maintenance, 4)
Preclinical disease model studies.
Research toward developing and providing proof of concept for cell therapy
approaches, particularly in animal models, strengthening the intellectual
property position of the core technologies, and the publication of high
impact research work. Program staff will be expected to attend national and
international conferences. The Department of Biochemistry is housed within
a purpose-built facility equipped with state-of-the-art facilities for cell
culture, microscopy and digital image analysis, protein microsequencing,
gene targeting, protein structure determination, and has close access to
FACS and confocal microscopy facilities and has a well equipped centralised
technical services unit.
Generous salary packages and conditions are to be offered.to highly
motivated Post-Doctoral Research Scientists in the following areas:
1. Cell Biologist with skills appropriate to the isolation and maintenance
of human pluripotent cell lines. Exposure to stem cell biology would be
helpful.
2. Protein Chemist to characterise bioactive factors with roles in cell
differentiation. Experience in receptor/ligand interaction or cell based
assays advantageous.
3. Cell Biologist/Reproductive Technologist to consider the mechanism of
genetic "reprogramming" as a consequence of nuclear transfer.
4. Cell Biologist to identify markers of germ cell development, which might
be targets for vertebrate pest control by immunological means.
5. Cell Biologist with skills appropriate to dissection of cell cycle
regulation in stem cells.
6. Developmental Biologist to characterise molecular control of cell
proliferation during mammalian embryonic development.
Applications should be received by March 15, 2000. For further details
contact: Professor Peter Rathjen, Peter.Rathjen@adelaide.edu.au (positions
1-4), or Dr Steven Dalton Steven.Dalton@adelaide.edu.au (positions 5,6) at
Department of Biochemistry, University of Adelaide, Adelaide 5005, South
Australia, Tel (61 8) 8303 5354, Fax (61 8) 8303 4348.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From dw235 at hermes.cam.ac.uk Tue Feb 29 09:01:01 2000
From: dw235 at hermes.cam.ac.uk (Denise Weekly)
Date: Wed Aug 20 11:55:27 2003
Subject: Superovulation times
Message-ID: <001c01bf8293$81038c40$fcbb6f83@cimr.cam.ac.uk>

Dear list members

I am currently setting up a transgenic services in our facility and have a question which I hope someone will be able to answer for me.
The injection times for superovulation work?
we currently inject C57CBA/F1 females at about 4-5 weeks (due to our own quarantine arrangement for the building) at 10am PMS and 47 hrs later we inject HCG (at 9am) the room light cycle is on at 6am and goes off at 8pm.
Users are now requesting that superovulation injections are carried out at 3pm and 47hrs later for the HCG, the reason for this being they have a better embryo viability and yield with these times, this will cause a few problems for us in the terms of weekend work.
Is anyone able to clarify the issue for us from personal experience and what their finding were/are.

Your help with this would be appreciated

Denise Weekly
Central Biomedical Services,
Addenbrookes Hospital Site,
Hills Road,
Cambridge,
CB2 2SP
Tel : 01223 336764

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From teresam at cmmt.ubc.ca Tue Feb 29 11:52:50 2000
From: teresam at cmmt.ubc.ca (Teresa McKernan)
Date: Wed Aug 20 11:55:27 2003
Subject: Helicobacter PCR testing
Message-ID: <v03007805b4e1d3808529@[142.231.21.201]>

Dear all:
I am scheduled to run a PCR tomorrow morning, but can't find my folder with
the helicobacter hepaticus PCR protocol.
Could someone please take the time to write out the PCR protocol (H.
hepaticus only) as well as write down which primers are used during (to
make sure I am have the same.)
Our PCR machine is drastically overused and if I miss tomorrow, I will have
to wait at least another week to get in - and don't have time to order the
article again.
Thanks in advance.
teresa

Centre for Molecular Medicine and Therapeutics
950 West 28th Avenue
University of British Columbia
Vancouver, BC
Tel: 604 875 3845
Fax: 604 875 3840
teresam@cmmt.ubc.ca

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From ssp at its.caltech.edu Tue Feb 29 12:23:17 2000
From: ssp at its.caltech.edu (Shirley Pease)
Date: Wed Aug 20 11:55:27 2003
Subject: Superovulation times
In-Reply-To: <001c01bf8293$81038c40$fcbb6f83@cimr.cam.ac.uk>
Message-ID: <Pine.GSO.4.05.10002291208050.14931-100000@sue>

Hi Denise,

We too have had occasion to adjust injection times. This has had to do
with finding a window of time within which to do the microinjection, at
which the embryos are optimally staged. There are a number of perameters
that can influence the onset of such a period of time. They include the
time at which hormones are given and the time at which the lights go out
at night. The onset of darkness is more influential than the start of
daylight time.

In working with blastocysts here, we wanted to inject in the afternoons.
But we found that the embryos, when harvested, were too advanced. So in
order to get to them "earlier", instead of bringing the injection time
forward, which woulad have caused some inconvenience in harvesting times,
etc, we moved the light cycle back. So lights go out at 9pm now, instead
of 7pm. Before we did that, we tried giving hormones later, without
altering the light cycle. So instead of giving hormones at midday, we
gave at 2pm. This didn't help us. The onset of darkness was more
influential in delaying the development of the embryos. Both perameters
are important. You may want to try altering injection times first and
resort to light cycle changes if you do not get what you need.

At the current time, we have all our embryo production stock on a leter
day. Lights come on at 7am and go off at 9pm. We dose with hormones at
1pm and 12m midday. This, fortunately, provides us with both blastocysts
and single cell embryos that are pretty much perfectly staged for
injection at 1pm onwards each day.

Hope some of this is helpful, at least!

Best, Shirley.

Shirley Pease,
Director, Transgenic and Knockout Core Facility,
California Institute of Technology,
Division of Biology, 147-75
Pasadena,
California, 91125
Tel 626 395 3996
Fax 626 449 0756

On Tue, 29 Feb 2000, Denise Weekly wrote:

> Dear list members
>
> I am currently setting up a transgenic services in our facility and have a question which I hope someone will be able to answer for me.
> The injection times for superovulation work?
> we currently inject C57CBA/F1 females at about 4-5 weeks (due to our own quarantine arrangement for the building) at 10am PMS and 47 hrs later we inject HCG (at 9am) the room light cycle is on at 6am and goes off at 8pm.
> Users are now requesting that superovulation injections are carried out at 3pm and 47hrs later for the HCG, the reason for this being they have a better embryo viability and yield with these times, this will cause a few problems for us in the terms of weekend work.
> Is anyone able to clarify the issue for us from personal experience and what their finding were/are.
>
> Your help with this would be appreciated
>
> Denise Weekly
> Central Biomedical Services,
> Addenbrookes Hospital Site,
> Hills Road,
> Cambridge,
> CB2 2SP
> Tel : 01223 336764
>
>
>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From cbenjamin at cvm.tamu.edu Tue Feb 29 16:56:57 2000
From: cbenjamin at cvm.tamu.edu (Cara Benjamin)
Date: Wed Aug 20 11:55:27 2003
Subject: microinjector
Message-ID: <s8bbfa73.069@cvm.tamu.edu>

List-

I have recently begun pronuclear injection into FVB donor embryos.
I am using an Eppendorf microinjector 5242 and am having difficulty
finding the proper injection and holding pressures. I would
appreciate any suggestions for a range to start working with.

Cara Benjamin
331 Vet Research Building - VAPH
College of Veterinary Medicine
Texas A&M university
College Station, TX 77843-4458

cbenjamin@cvm.tamu.edu

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From tjf at uci.edu Tue Feb 29 18:45:09 2000
From: tjf at uci.edu (Tom Fielder)
Date: Wed Aug 20 11:55:27 2003
Subject: best ES cell source
Message-ID: <v04003a09b4e1c3a22e52@[128.200.120.196]>

Anyone have an opinion as to the best commercial source of ES cells?

Tom

*******************************************
Thomas J. Fielder, M.A., Specialist
Transgenic Mouse Facility
UC-Irvine
University Lab Animal Resources
147 BSA
Irvine, CA 92697-1310
email: tjf@uci.edu
phone: 949-824-8579
fax: 949-824-2003
http://darwin.bio.uci.edu/~tjf/index.html
*******************************************

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From nagy at mshri.on.ca Wed Feb 2 15:27:20 2000
From: nagy at mshri.on.ca (Andras Nagy)
Date: Fri Jul 9 14:05:14 2004
Subject: your input is needed
Message-ID: <38984CD7.429E0A59@mshri.on.ca>

Dear List,

Thanks very much for your contribution, which will be properly
acknowledged.

Andras Nagy, PhD
Senior Staff Scientist
Mount Sinai Hospital
Samuel Lunenfeld Research Institute
600 University Ave.
Toronto, M5G 1X5
Canada

Email: nagy@mshri.on.ca
tel. (416)-586-3246 0ffice
tel. (416)-586-8455 Lab
tel. (416)-586-5361 Secretary
fax (416)-586-8588
URL: http://www.mshri.on.ca/nagy/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Wed Feb 2 19:05:54 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Fri Jul 9 14:05:14 2004
Subject: 3rd edition of Hogan manual
References: <38984CD7.429E0A59@mshri.on.ca>
Message-ID: <38988012.501677BD@karmanos.org>

Andras Nagy wrote:

> I am contributing to the new, third edition of the Brigid Hogan's:
> "Manipulating the Mouse Embryo; A laboratory manual". The plan is to
> make a significant rewriting on the book...

Other vector sequences (such as pPNT) are hard to come by, except in obscure
web sites such as: http://cmmg.biosci.wayne.edu/asg/pPNT

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From f.lin at victorchang.unsw.edu.au Thu Feb 3 07:25:33 2000
From: f.lin at victorchang.unsw.edu.au (fanglin)
Date: Fri Jul 9 14:05:15 2004
Subject: animals survival and exercise tolerance
Message-ID: <200002030628.RAA22223@gimr.garvan.unsw.edu.au>

Dear All,

We consider to set up animals(mice) survival(natural) and exercise
tolerance experiments. However, I don't any experience on these and would
like to have your suggestions, advice and protocols.

Thanks very much in advance.

Fang

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From k.j.armour at abdn.ac.uk Thu Feb 3 10:09:50 2000
From: k.j.armour at abdn.ac.uk (Kenneth J. Armour)
Date: Fri Jul 9 14:05:15 2004
Subject: 3rd edition of Hogan manual
In-Reply-To: <38988012.501677BD@karmanos.org>
References: <38984CD7.429E0A59@mshri.on.ca>
Message-ID: <l03130301b4bf01f69c6b@[139.133.139.185]>

Andras Nagy wrote:

> I am contributing to the new, third edition of the Brigid Hogan's:
> "Manipulating the Mouse Embryo; A laboratory manual". The plan is to
> make a significant rewriting on the book...

Anton Scott Goustin wrote:

Regards

Ken Armour

-o-o-o-o-o-

Kenneth J. Armour Ph.D.
Bone Research Group
Dept. of Medicine and Therapeutics
University of Aberdeen
Polwarth Building
Foresterhill
Aberdeen AB25 2ZD, U.K.

Tel: +44(0)1224 551283
Tel: +44(0)1224 554498/553027 (labs)
Fax: +44 (0) 1224 699884

<mailto: k.j.armour@abdn.ac.uk>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From maria.alexiou at csc.mrc.ac.uk Thu Feb 3 10:17:50 2000
From: maria.alexiou at csc.mrc.ac.uk (Maria Alexiou)
Date: Fri Jul 9 14:05:15 2004
Subject: No subject
Message-ID: <v01540b02b4bf05f6049f@[146.179.68.52]>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Lutz.Bunger at ed.ac.uk Thu Feb 3 13:08:20 2000
From: Lutz.Bunger at ed.ac.uk (Lutz Bunger)
Date: Fri Jul 9 14:05:15 2004
Subject: animals survival and exercise tolerance
In-Reply-To: <200002030628.RAA22223@gimr.garvan.unsw.edu.au>
Message-ID: <200002031308.NAA28153@holyrood.ed.ac.uk>

Lutz Bunger
ICAPB, Kings Buildings
The University of Edinburgh
Edinburgh EH9 3JT
Scotland

Tel 44 (0)131 650 5442
Fax 44 (0)131 667 3210
Email Lutz.Bunger@ed.ac.uk
http://www.ed.ac.uk/~eang17/mice.html
http://helios.bto.ed.ac.uk/icapb/staff/frames.html

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From mmichalkiewicz at hsc.wvu.edu Thu Feb 3 15:28:17 2000
From: mmichalkiewicz at hsc.wvu.edu (Mike Michalkiewicz)
Date: Fri Jul 9 14:05:15 2004
Subject: your input is needed
Message-ID: <s8995860.003@mail.hsc.wvu.edu>

Regards,

Mike Michalkiewicz, DVM, PhD

West Virginia University
Department of Physiology
PO Box 9229
Morgantown, WV 26506
phone 304 293 1515; Fax 304 293 3850
http://www.hsc.wvu.edu/neurosci/faculty/michalkiewicz.html

>>> Andras Nagy <nagy@mshri.on.ca> 02/02/00 10:27AM >>>
Dear List,

Thanks very much for your contribution, which will be properly
acknowledged.

Andras Nagy, PhD
Senior Staff Scientist
Mount Sinai Hospital
Samuel Lunenfeld Research Institute
600 University Ave.
Toronto, M5G 1X5
Canada

Email: nagy@mshri.on.ca
tel. (416)-586-3246 0ffice
tel. (416)-586-8455 Lab
tel. (416)-586-5361 Secretary
fax (416)-586-8588
URL: http://www.mshri.on.ca/nagy/

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From pasceri at sickkids.on.ca Thu Feb 3 16:42:48 2000
From: pasceri at sickkids.on.ca (Peter Pasceri)
Date: Fri Jul 9 14:05:15 2004
Subject: NOD mice
Message-ID: <l03130301b4bf5b0b1e6a@[142.20.155.242]>

Good luck.
Peter

--
Peter Pasceri
Program of Developmental Biology
The Hospital for Sick Children
555 University Avenue
Toronto, Ontario, Canada, M5G-1X8

Phone: ( 416 ) 813-8169
Fax: ( 416 ) 813-8883
E-mail: pasceri@sickkids.on.ca

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From jscott at wehi.EDU.AU Thu Feb 3 21:17:15 2000
From: jscott at wehi.EDU.AU (Julie Scott)
Date: Fri Jul 9 14:05:15 2004
Subject: your mail
In-Reply-To: <v01540b02b4bf05f6049f@[146.179.68.52]>
Message-ID: <Pine.GSO.4.05.10002040747190.23343-100000@wehiz.wehi.EDU.AU>

On Thu, 3 Feb 2000, Maria Alexiou wrote:

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From saboulard at hotmail.com Fri Feb 4 11:29:22 2000
From: saboulard at hotmail.com (didier SABOULARD)
Date: Fri Jul 9 14:05:15 2004
Subject: HPV-16 / GM-CSF
Message-ID: <20000204102922.69110.qmail@hotmail.com>

Dear All,

Dr DIDIER SABOULARD
LABORATORY OF PATHOLOGY
CHU Liege, Belgium
saboulard@hotmail.com
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From parker-thornburg.1 at osu.edu Fri Feb 4 22:44:13 2000
From: parker-thornburg.1 at osu.edu (Jan Parker-Thornburg)
Date: Fri Jul 9 14:05:15 2004
Subject: swimming mice
Message-ID: <v04210102b4c0fe16234d@[128.146.132.236]>

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Linda.Watkins at stjude.org Fri Feb 4 20:00:40 2000
From: Linda.Watkins at stjude.org (Watkins, Linda )
Date: Fri Jul 9 14:05:15 2004
Subject: caspase 8 mice
Message-ID: <83B4CF76D818D111982000805F31CA5302D144A8@sjmemexc1.stjude.org>

Dear List Members,

I have been tasked to find a CASPASE 8 mouse source... anyone knowing of
such a source for this mouse, please contact me... I would surely appreciate
it......

Linda Watkins

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From szhao at bcm.tmc.edu Fri Feb 4 20:05:45 2000
From: szhao at bcm.tmc.edu (Shulei Zhao)
Date: Fri Jul 9 14:05:15 2004
Subject: promoter for targeting choroid plexus
Message-ID: <Pine.GSO.4.10.10002041404560.23365-100000@watson.bcm.tmc.edu>

Shulei Zhao
Dept of Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX 77030

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From goustina at karmanos.org Fri Feb 4 23:13:44 2000
From: goustina at karmanos.org (Anton Scott Goustin)
Date: Fri Jul 9 14:05:15 2004
Subject: Hogan III and NT technology
References: <38984CD7.429E0A59@mshri.on.ca>
Message-ID: <389B5D27.6F82724F@karmanos.org>

Andras Nagy wrote:

It would be wonderful to see this technology "catch on", and Hogan III could
certainly catalyze this technology spread. I would love to see a chapter
devoted to NT.

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From milstone at rascal.med.harvard.edu Sun Feb 6 03:10:11 2000
From: milstone at rascal.med.harvard.edu (David Milstone)
Date: Fri Jul 9 14:05:15 2004
Subject: promoter for targeting choroid plexus
In-Reply-To: <E12Gp8D-0005HG-00@tomatin.cc.ic.ac.uk>
References: <E12Gp8D-0005HG-00@tomatin.cc.ic.ac.uk>
Message-ID: <v0420550ab4c293da97c9@[134.174.88.145]>

My impression from casual conversations is that *many* transgenes are
expressed in choroid plexus, as if it were "the testis of the CNS".

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Steven.Sansing at bms.com Mon Feb 7 13:10:27 2000
From: Steven.Sansing at bms.com (Steven R Sansing)
Date: Fri Jul 9 14:05:15 2004
Subject: non-transgenic animals
Message-ID: <389EC443.377D2FD8@bms.com>

Steve Sansing
Bristol-Myers Squibb
Wallingford, Ct
steven.sansing@bms.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Steven.Sansing at bms.com Mon Feb 7 14:03:18 2000
From: Steven.Sansing at bms.com (Steven R Sansing)
Date: Fri Jul 9 14:05:15 2004
Subject: non-transgenic use
Message-ID: <389ED0A6.55A0B14B@bms.com>

All,

I've started receiving some responses and it appears that there's
some confusion over what i'm asking. Hopefully this will help.

Are people using their "negative" animals? (either transgenic or
knockout). OR are the animals always euthanatized?

IF people were requested to use the "negative" animals what would
be some concerns?

Again i hope this clears up some of the confusion and thanks
again....

Steve Sansing
Bristol-Myers Squibb
Wallingford, CT
steven.sansing@bms.com

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From pozzi at crs.ifo.it Tue Feb 8 11:11:21 2000
From: pozzi at crs.ifo.it (Laura Pozzi)
Date: Fri Jul 9 14:05:15 2004
Subject: No subject
Message-ID: <3.0.1.32.20000208121121.0068fc5c@crs.ifo.it>

Laura
Dr.Laura Pozzi
Lab.Modelli Animali
Centro Ricerca Sperimentale
Istituto Regina Elena
Via delle Messi d'oro 156
00158 Roma, Italy
tel. -39 06 49852512
fax - 39 06 49852505
email pozzi@crs.ifo.it

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From elena.notarianni at ncl.ac.uk Tue Feb 8 10:48:48 2000
From: elena.notarianni at ncl.ac.uk (Elena Notarianni)
Date: Fri Jul 9 14:05:15 2004
Subject: No subject
Message-ID: <200002081147.LAA20031@cheviot1.ncl.ac.uk>

Dear List,

Does anyone know of a source of STO cells with an FGF2 knock-out?
Thanks, Elena.

Dr. Elena Notarianni,
Comparative Biology Centre,
The Medical School,
Newcastle University,
Framlington Place,
Newcastle Upon Tyne NE2 4HH.
Tel: 0191 222 8817 or 6715
FAX: 0191 222 8688.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From sosuka at mr-envi.med.osaka-u.ac.jp Tue Feb 8 12:03:59 2000
From: sosuka at mr-envi.med.osaka-u.ac.jp (Soh F. Osuka)
Date: Fri Jul 9 14:05:15 2004
Subject: To the KO-logists community,
In-Reply-To: <3.0.1.32.20000208121121.0068fc5c@crs.ifo.it>
Message-ID: <200002081223.VAA18848@mr-envi.med.osaka-u.ac.jp>

Dear Laura

What is the source of neo gene?

In my experience, I got a very low number of G418 resistant colonies when I
use mutated neo from pPNT to target one gene, while the other lab member is
OK for other targeting with it.

After a replacement of mutated neo with wild-type one from
pMC1neo(Stratagene) in the targeting vector, the problem was solved.

Thanks,

Soh

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Biborka.BVeress at cmb.ki.se Tue Feb 8 14:26:50 2000
From: Biborka.BVeress at cmb.ki.se (Biborka Bereczky Veress , Genetik)
Date: Fri Jul 9 14:05:15 2004
Subject: Hogan III and NT technology
Message-ID: <l03110704b4c5d67046e4@[130.237.120.40]>

************************************************************

Biborka Bereczky Veress
research engineer
Karolinska Institutet
CMB/MouseCamp
Von Eulers v?g 3
171 77 Stockholm

Tel. office: + 46 8 728 73 26
Tel. lab.: + 46 8 728 76 13, 75 14
Fax: + 46 8 348135
e-mail: Biborka.BVeress@cmb.ki.se

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From junming at physio1.utmem.edu Wed Feb 9 01:45:46 2000
From: junming at physio1.utmem.edu (Junming Yue)
Date: Fri Jul 9 14:05:15 2004
Subject: EGFP
Message-ID: <l03130301b4c676f88929@[132.192.44.66]>

Dear Dieter,

Could you send me your mail address again?

Junming Yue

"transgenic-list" web site (archives, FAQs, protocols, links) address:
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From tjf at uci.edu Tue Feb 8 17:06:03 2000
From: tjf at uci.edu (Tom Fielder)
Date: Fri Jul 9 14:05:15 2004
Subject: cryopreservation fees survey
Message-ID: <v04003a06b4c58bd8db0e@[128.200.21.145]>

Hi all,

What do you charge for freezing 8-cell stage mouse embryos?

How many donors and/or studs do you ask the client to supply?

How do you recover the storage costs (e.g., liquid nitrogen, labor, etc.) -
is this billed on a yearly basis, or do you charge it as one lump sum up
front?

What do you charge for reconstituting a frozen line? Is this billed as a
separate service?

Do your fees vary depending on the strain of mouse?

Thanks in advance.

Tom

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

************************************************************

Biborka Bereczky Veress
research engineer
Karolinska Institutet
CMB/MouseCamp
Von Eulers v?g 3
171 77 Stockholm

Tel. office: + 46 8 728 73 26
Tel. lab.: + 46 8 728 76 13, 75 14
Fax: + 46 8 348135
e-mail: Biborka.BVeress@cmb.ki.se

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From tjf at uci.edu Tue Feb 8 17:06:03 2000
From: tjf at uci.edu (Tom Fielder)
Date: Fri Jul 9 14:05:15 2004
Subject: cryopreservation fees survey
Message-ID: <v04003a06b4c58bd8db0e@[128.200.21.145]>

Hi all,

What do you charge for freezing 8-cell stage mouse embryos?

How many donors and/or studs do you ask the client to supply?

How do you recover the storage costs (e.g., liquid nitrogen, labor, etc.) -
is this billed on a yearly basis, or do you charge it as one lump sum up
front?

What do you charge for reconstituting a frozen line? Is this billed as a
separate service?

Do your fees vary depending on the strain of mouse?

Thanks in advance.

Tom

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

Dear List,

Does anyone know of a source of STO cells with an FGF2 knock-out?
Thanks, Elena.

Dr. Elena Notarianni,
Comparative Biology Centre,
The Medical School,
Newcastle University,
Framlington Place,
Newcastle Upon Tyne NE2 4HH.
Tel: 0191 222 8817 or 6715
FAX: 0191 222 8688.

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From pozzi at crs.ifo.it Tue Feb 8 11:11:21 2000
From: pozzi at crs.ifo.it (Laura Pozzi)
Date: Fri Jul 9 14:05:15 2004
Subject: No subject
Message-ID: <3.0.1.32.20000208121121.0068fc5c@crs.ifo.it>

Laura
Dr.Laura Pozzi
Lab.Modelli Animali
Centro Ricerca Sperimentale
Istituto Regina Elena
Via delle Messi d'oro 156
00158 Roma, Italy
tel. -39 06 49852512
fax - 39 06 49852505
email pozzi@crs.ifo.it

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

Dear Dieter,

Could you send me your mail address again?

Junming Yue

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

Dear Laura

What is the source of neo gene?

In my experience, I got a very low number of G418 resistant colonies when I
use mutated neo from pPNT to target one gene, while the other lab member is
OK for other targeting with it.

After a replacement of mutated neo with wild-type one from
pMC1neo(Stratagene) in the targeting vector, the problem was solved.

Thanks,

Soh

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From Andrew.Davidson at med.monash.edu.au Wed Feb 9 00:48:22 2000
From: Andrew.Davidson at med.monash.edu.au (DR AD DAVIDSON [MIC])
Date: Fri Jul 9 14:05:15 2004
Subject: effects of duplicated vector sequences on integration
Message-ID: <CCBBBE737F@its-med.cc.monash.edu.au>

Hi all,

I have a question regarding the effects of duplicated vector
sequences on transgene integration.

thanks in advance,

"transgenic-list" web site (archives, FAQs, protocols, links) address:
http://www.med.ic.ac.uk/db/dbbm/tglist.htm

From icta2000 at hotmail.com Wed Feb 9 12:24:35 2000
From: icta2000 at hotmail.com (ICTA 2000)
Date: Fri Jul 9 14:05:15 2004
Subject: 3rd ICTA.
Message-ID: <20000209122435.36296.qmail@hotmail.com>