Success Rates

The Case Transgenic and Targeting Facility has an outstanding record of success in making transgenic and knockout mice. Our goals are to produce a minimum of three transgenic founder mice per construct, three strong chimeric mice per cell line, three targeted cell lines per construct and sufficient rederived mice to re-establish the strain quickly. We exceed these goals for the majority of orders.

success rates


CRISPR/Cas Success Rates

     We have completed over 100 CRISPR knockin/out projects. The vast majority of projects have generated multiple live founder mice with the desired allele. The founder mice are not mosaic for the founder allele, unlike in early applications of this technology. We have successfully generated many knockout, point mutation knockins, reporter/tag/Cre knockins, floxed alleles, Rosa26 and H11 safe harbor targeted transgenes, and floxed-stop alleles.



ISTT SURVEY ON PRODUCTION OF TRANSGENIC MICE
Thomas J. Fielder, Laura Barrios, Lluís Montoliu
September 2012
pca PCA Graph 1
"This is a two-dimensional graph that was made by projecting the mean of the individual facility in relation to Principal Components 1 and 2.

In this graph, the X-axis (corresponding to Principal Component 1) is interpreted as “Transgenesis efficiency” or “Transgenic pups obtained”. Facilities located to the right have better values than those located to the left. The Y-axis (corresponding to Principal Component 2) is interpreted as “embryo transfer efficiency” or “Pups born”. Facilities located above zero have better values than those located below. The ideal position for a facility is in the upper right quadrant. Facilities showing the best performance regarding the ratios considered will be found in this upper right corner.

Your facility is depicted as an upside down blue triangle while the other facilities are depicted as green open circles.

According to the position of your facility, the following recommendations should be considered. If your facility is located in the upper right quadrant, don’t change anything. If your facility is located in the upper left quadrant, total pup yields are good, but you may be able to improve your transgenic pup yields. If your facility is located in the lower right quadrant, transgenic pup yields are good, but you may be able to improve your total pup yields (e.g., by improving the embryo-transfer procedure). Finally, if your facility is located in the lower left quadrant, you may be able to improve both total pup and transgenic pup yields."