We have completed over 100 CRISPR knockin/out projects. The vast majority of projects have generated multiple live founder mice with the desired allele. The founder mice are not mosaic for the founder allele, unlike in early applications of this technology. We have successfully generated many knockout, point mutation knockins, reporter/tag/Cre knockins, floxed alleles, Rosa26 and H11 safe harbor targeted transgenes, and floxed-stop alleles.
ISTT SURVEY ON PRODUCTION OF TRANSGENIC MICE
Thomas J. Fielder, Laura Barrios, Lluís Montoliu
PCA Graph 1
"This is a two-dimensional graph that was made by projecting the mean of the
individual facility in relation to Principal Components 1 and 2.
In this graph, the X-axis (corresponding to Principal Component 1) is interpreted
as “Transgenesis efficiency” or “Transgenic pups obtained”. Facilities located to
the right have better values than those located to the left. The Y-axis
(corresponding to Principal Component 2) is interpreted as “embryo transfer
efficiency” or “Pups born”. Facilities located above zero have better values than
those located below. The ideal position for a facility is in the upper right
quadrant. Facilities showing the best performance regarding the ratios
considered will be found in this upper right corner.
Your facility is depicted as an upside down blue triangle while the other
facilities are depicted as green open circles.
According to the position of your facility, the following recommendations should
be considered. If your facility is located in the upper right quadrant, don’t
change anything. If your facility is located in the upper left quadrant, total pup
yields are good, but you may be able to improve your transgenic pup yields. If
your facility is located in the lower right quadrant, transgenic pup yields are
good, but you may be able to improve your total pup yields (e.g., by improving
the embryo-transfer procedure). Finally, if your facility is located in the lower left
quadrant, you may be able to improve both total pup and transgenic pup yields."