Sperm Cryopreservation

What You Will Provide What We Will Provide Cost Timeline

Online Ordering

You will need the following information to order
  • PI name, address and contact info
  • Account number
  • Genetic background
  • Gene, transgene or trait
  • Age of males
  • Location of mice (building and room)

Frequently Asked Questions

Can I store the samples in our lab liquid nitrogen freezer?
     We do not normally allow labs to store their own samples. Unlike tissue culture cells, cryopreserved sperm are highly sensitive to small changes in temperature, such that even briefly pulling canisters to find tubes can irreparably damage embryos.

Sperm cryopreservation
     We use the 2008 Ostermeirer et al "Jax protocol" for sperm cryopreservation. This procedure works for sperm from the common inbred strains. Sperm cryopreservation does not have the long track record of embryo cryopreservation, so the reliability is not as established.

     Despite the improvement of sperm cryopreservation and IVF protocols, the inbred nature of most mouse strains means that we need two males of the same strain of proven fertility to ensure success.

     For some inbred strains with particularly poor superovulation--for example, BALB/c--reanimation may require that eggs from another strain be used for in vitro fertilization.

     The fertility of the specific males to be used for crypreservation must be documented.

In vitro fertilization for reanimation.